PMID- 30310311
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220321
IS  - 1178-7090 (Print)
IS  - 1178-7090 (Electronic)
IS  - 1178-7090 (Linking)
VI  - 11
DP  - 2018
TI  - PKCbetaII-induced upregulation of PGP9.5 and VEGF in postoperative persistent pain 
      in rats.
PG  - 2095-2106
LID - 10.2147/JPR.S144852 [doi]
AB  - PURPOSE: Postoperative pain is a common clinical problem. In this study, we aimed 
      to investigate the role of protein kinase C betaII (PKCbetaII) in the progression of 
      postoperative pain following skin/muscle incision and retraction (SMIR) surgery. 
      MATERIALS AND METHODS: SMIR postoperative pain model was established in rats, 
      akin to a clinical procedure. The expression level and location of p-PKCbetaII were 
      observed in dorsal root ganglion (DRG) or spinal cord from SMIR-operated rats by 
      Western blotting and immunofluorescence. In addition, the effects of PKCbetaII on 
      the expression of protein gene product 9.5 (PGP9.5) or vascular endothelial 
      growth factor (VEGF) were assessed by using pharmacological activator and 
      inhibitor of PKCbetaII. Moreover, mechanical withdrawal threshold (MWT) was assessed 
      before or after SMIR-operated rats were treated with inhibitor or activator of 
      PKCbetaII. RESULTS: The expression of PKCbetaII in DRG and spinal cord was 
      significantly increased after SMIR surgery (P < 0.001, P < 0.01) and expression 
      of PKCbetaII was located in the neurons of the spinal cord, and magnocellular 
      neurons, non-peptide neurons, and peptide neurons in DRG. Besides, compared with 
      skin/muscle incision group, retraction caused a marked increase in the expression 
      of PKCbetaII and a significant decrease of MWT (P < 0.001, P < 0.05). The activator 
      of PKCbetaII greatly increased the expression of PGP9.5 and VEGF (P < 0.05, P < 
      0.01) and enhanced MWT (P < 0.001), while inhibitor of PKCbetaII decreased the 
      expression of PGP9.5 and VEGF and attenuated MWT (P < 0.05, P < 0.01, P < 0.001). 
      CONCLUSION: Activation of PKCbetaII signaling pathways might be an important 
      mechanism in the progression of postoperative pain.
FAU - Zhu, Xiang
AU  - Zhu X
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, People's Republic of China, liuyuxi919@163.com; 
      su1_cao123@163.com.
FAU - Liu, Yuxi
AU  - Liu Y
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, People's Republic of China, liuyuxi919@163.com; 
      su1_cao123@163.com.
FAU - Huang, Hongfang
AU  - Huang H
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, People's Republic of China, liuyuxi919@163.com; 
      su1_cao123@163.com.
FAU - Zhang, Yonghua
AU  - Zhang Y
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, People's Republic of China, liuyuxi919@163.com; 
      su1_cao123@163.com.
FAU - Huang, Saisai
AU  - Huang S
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, People's Republic of China, liuyuxi919@163.com; 
      su1_cao123@163.com.
FAU - Zhou, Weiwei
AU  - Zhou W
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, People's Republic of China, liuyuxi919@163.com; 
      su1_cao123@163.com.
FAU - Bian, Xiaocui
AU  - Bian X
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, People's Republic of China, liuyuxi919@163.com; 
      su1_cao123@163.com.
FAU - Shen, Shiren
AU  - Shen S
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, People's Republic of China, liuyuxi919@163.com; 
      su1_cao123@163.com.
FAU - Cao, Su
AU  - Cao S
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, People's Republic of China, liuyuxi919@163.com; 
      su1_cao123@163.com.
LA  - eng
PT  - Journal Article
DEP - 20180927
PL  - New Zealand
TA  - J Pain Res
JT  - Journal of pain research
JID - 101540514
PMC - PMC6166760
OTO - NOTNLM
OT  - PGP9.5
OT  - PKCbetaII
OT  - VEGF
OT  - neurons
OT  - postoperative persistent pain
COIS- Disclosure The authors report no conflicts of interest in this work.
EDAT- 2018/10/13 06:00
MHDA- 2018/10/13 06:01
PMCR- 2018/09/27
CRDT- 2018/10/13 06:00
PHST- 2018/10/13 06:00 [entrez]
PHST- 2018/10/13 06:00 [pubmed]
PHST- 2018/10/13 06:01 [medline]
PHST- 2018/09/27 00:00 [pmc-release]
AID - jpr-11-2095 [pii]
AID - 10.2147/JPR.S144852 [doi]
PST - epublish
SO  - J Pain Res. 2018 Sep 27;11:2095-2106. doi: 10.2147/JPR.S144852. eCollection 2018.