PMID- 30315952
OWN - NLM
STAT- MEDLINE
DCOM- 20181109
LR  - 20181109
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 299
DP  - 2018 Dec 15
TI  - In utero exposure to hexavalent chromium disrupts rat fetal testis development.
PG  - 201-209
LID - S0378-4274(18)31993-3 [pii]
LID - 10.1016/j.toxlet.2018.10.010 [doi]
AB  - Hexavalent chromium (Cr(6+)) acts as an endocrine disruptor. Herein, we 
      investigated effects of Cr(6+) on the development of rat fetal Leydig and Sertoli 
      cells, which support differentiation of the male reproductive tract in late 
      gestation. Female pregnant Sprague Dawley rats were gavaged with potassium 
      dichromate (0, 3, 6, and 12 mg/kg) from gestational days (GD) 12 to GD 21. Leydig 
      and Sertoli cell function was evaluated by investigating serum testosterone 
      levels, cell number and distribution, and the expression levels of Leydig and 
      Sertoli cell genes and proteins. Cr(6+) increased serum testosterone level at 
      dose of 3 mg/kg (1.170 +/- 0.121 ng/ml vs. 0.720 +/- 0.082 ng/ml in the control), 
      while lowered it at dose of 12 mg/kg (0.400 +/- 0.098 ng/ml). In addition, it 
      showed that Cr(6+) dose-dependently reduced Leydig cell size and cytoplasmic size 
      and decreased the percentage of medium fetal Leydig cell cluster at dose of 
      12 mg/kg. Further study demonstrated that the expression of Leydig cell (Lhcgr, 
      Scarb1, and Hsd3b1) and Sertoli cell (Fshr, Pdgfa, and Lif) genes in the testis 
      was upregulated at dose of 3 mg/kg while the expression of Lhcgr, Hsd17b3 and 
      Igf1 was downregulated by Cr(6+) at dose of 12 mg/kg. In conclusion, Cr(6+) had 
      biphasic effects on fetal Leydig cell development with low dose to stimulate 
      testosterone production and high dose to inhibit it, possibly via biphasically 
      regulating growth factor gene expression in fetal Sertoli cells.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Zheng, Wenwen
AU  - Zheng W
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China.
FAU - Ge, Fei
AU  - Ge F
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China.
FAU - Wu, Keyang
AU  - Wu K
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China.
FAU - Chen, Xianwu
AU  - Chen X
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China.
FAU - Li, Xiaoheng
AU  - Li X
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China.
FAU - Chen, Yong
AU  - Chen Y
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China.
FAU - Lv, Yao
AU  - Lv Y
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China.
FAU - Lian, Qingquan
AU  - Lian Q
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China. Electronic address: lianqingquanmz@163.com.
FAU - Ge, Ren-Shan
AU  - Ge RS
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      China. Electronic address: r_ge@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20181010
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Endocrine Disruptors)
RN  - 0R0008Q3JB (Chromium)
RN  - 18540-29-9 (chromium hexavalent ion)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Animals
MH  - Chromium/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Endocrine Disruptors/*toxicity
MH  - Female
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Leydig Cells/drug effects/metabolism
MH  - Male
MH  - Maternal Exposure/*adverse effects
MH  - Organogenesis/*drug effects/genetics
MH  - Pregnancy
MH  - Rats, Sprague-Dawley
MH  - Sertoli Cells/drug effects/metabolism
MH  - Testis/*drug effects/embryology
MH  - Testosterone/metabolism
OTO - NOTNLM
OT  - Cell and cytoplasmic size
OT  - Cell distribution
OT  - Fetal Leydig cells
OT  - Fetal Sertoli cell
OT  - Hexavalent chromium
OT  - Testosterone
EDAT- 2018/10/14 06:00
MHDA- 2018/11/10 06:00
CRDT- 2018/10/14 06:00
PHST- 2018/05/31 00:00 [received]
PHST- 2018/09/30 00:00 [revised]
PHST- 2018/10/08 00:00 [accepted]
PHST- 2018/10/14 06:00 [pubmed]
PHST- 2018/11/10 06:00 [medline]
PHST- 2018/10/14 06:00 [entrez]
AID - S0378-4274(18)31993-3 [pii]
AID - 10.1016/j.toxlet.2018.10.010 [doi]
PST - ppublish
SO  - Toxicol Lett. 2018 Dec 15;299:201-209. doi: 10.1016/j.toxlet.2018.10.010. Epub 
      2018 Oct 10.