PMID- 30316015
OWN - NLM
STAT- MEDLINE
DCOM- 20190131
LR  - 20190131
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 121
DP  - 2018 Dec
TI  - Comparison of six commercial ELISAs for the detection of antibodies against 
      porcine reproductive and respiratory syndrome virus (PRRSV) in field serum 
      samples.
PG  - 40-45
LID - S0034-5288(18)31337-7 [pii]
LID - 10.1016/j.rvsc.2018.10.005 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) is one of the most common 
      infectious diseases of swine globally. Since the course of PRRS virus (PRRSV) 
      infection is subclinical, laboratory diagnosis is necessary to detect the virus 
      or specific antibodies. The aim of this study was to assess the sensitivity and 
      specificity of IDEXX PRRS X3 Ab Test (IDEXX, USA), Civtest Suis E/S (Hipra, 
      Spain), INgezim PRRS 2.0 (Ingenasa, Spain), VetExpert PRRS Ab ELISA 4.0 (BioNote, 
      Korea), Pigtype PRRSV Ab (Qiagen, Germany) and PrioCHECK PRRSV Antibody ELISA 
      (ThermoFisher, USA), using serum samples obtained from 5 conventional 
      PRRSV-positive and 5 PRRSV-negative Polish pig farms. Specificity of ELISAs 
      ranged from 94.2% (ThermoFisher) to 100% (IDEXX and Hipra). ThermoFisher ELISA 
      had the highest detection rate and detected 67.2% samples from PRRSV-positive 
      farms as positive but considering its low specificity some of the positive 
      results may be incorrect. IDEXX ELISA considered as a reference detected 64.8% 
      positive sera in PRRSV-positive farms. On the other hand Hipra Elisa identified 
      only 51.8% of samples as positive. The diagnostic sensitivity of five ELISAs 
      relative to IDEXX ranged from 80.3% (Hipra) to 96.3% (ThermoFisher). Our study 
      showed significant differences in specificity and diagnostic sensitivity between 
      the compared kits. The differences in the performance appeared to be practically 
      negligible on farms where early infection with PRRSV occurred. However, on 
      PRRSV-negative farms, or farms with PRRSV stable sow herds, some ELISAs can give 
      results not reflecting the infection status in specific age groups.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Biernacka, Kinga
AU  - Biernacka K
AD  - Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary 
      Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, 02-776 
      Warsaw, Poland.
FAU - Podgorska, Katarzyna
AU  - Podgorska K
AD  - Department of Swine Diseases, National Veterinary Research Institute, Partyzantow 
      57, 24-100 Pulawy, Poland. Electronic address: 
      katarzyna.podgorska@piwet.pulawy.pl.
FAU - Tyszka, Anna
AU  - Tyszka A
AD  - Veterinary Diagnostic Laboratory, Vet Lab Group, Ostrodzka 46, 11-036 Gietrzwald, 
      Poland. Electronic address: wdl.serologia@vetlabgroup.pl.
FAU - Stadejek, Tomasz
AU  - Stadejek T
AD  - Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary 
      Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, 02-776 
      Warsaw, Poland. Electronic address: tomasz_stadejek@sggw.pl.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20181006
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Enzyme-Linked Immunosorbent Assay/methods/*veterinary
MH  - Female
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/virology
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*isolation & 
      purification
MH  - Sensitivity and Specificity
MH  - Swine
OTO - NOTNLM
OT  - Comparison
OT  - ELISA
OT  - PRRSV
OT  - Serum
OT  - Swine
EDAT- 2018/10/14 06:00
MHDA- 2019/02/01 06:00
CRDT- 2018/10/14 06:00
PHST- 2018/07/09 00:00 [received]
PHST- 2018/10/03 00:00 [revised]
PHST- 2018/10/05 00:00 [accepted]
PHST- 2018/10/14 06:00 [pubmed]
PHST- 2019/02/01 06:00 [medline]
PHST- 2018/10/14 06:00 [entrez]
AID - S0034-5288(18)31337-7 [pii]
AID - 10.1016/j.rvsc.2018.10.005 [doi]
PST - ppublish
SO  - Res Vet Sci. 2018 Dec;121:40-45. doi: 10.1016/j.rvsc.2018.10.005. Epub 2018 Oct 
      6.