PMID- 30316322
OWN - NLM
STAT- MEDLINE
DCOM- 20190930
LR  - 20190930
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Print)
IS  - 0143-4004 (Linking)
VI  - 70
DP  - 2018 Oct
TI  - Associations between fetal size, sex and both proliferation and apoptosis at the 
      porcine feto-maternal interface.
PG  - 15-24
LID - S0143-4004(18)30279-0 [pii]
LID - 10.1016/j.placenta.2018.08.006 [doi]
AB  - INTRODUCTION: Inadequate fetal growth has severe consequences for both neonatal 
      and adult development. It is hypothesised that the feto-maternal interface 
      associated with the lightest and male fetuses will undergo more apoptosis and 
      less proliferation than those supplying the closest to mean litter weight (CTMLW) 
      and female fetuses respectively. METHODS: Placental and endometrial samples 
      associated with the lightest and CTMLW (gestational day (GD) 18 and 30), male and 
      female (GD45, 60 and 90) Large White X Landrace conceptuses or fetuses were 
      obtained. The mRNA expression of candidate genes involved in apoptosis or 
      proliferation (BAX, BCL2, P53 and KI67) was quantified by qPCR. TUNEL staining 
      was performed on placental samples supplying the lightest and CTMLW fetuses (GD45 
      and 60), of both sex (GD60). RESULTS: Placentas associated with the lightest 
      fetuses had decreased P53 and KI67 expression compared to the CTMLW fetuses at 
      GD45. At GD60, P53 expression was increased in placentas supplying the lightest 
      compared to CTMLW fetuses. P53 expression was increased in endometrial samples 
      associated with the lightest compared to the CTMLW fetuses at GD45. At GD30 and 
      GD60 respectively, BAX expression was increased and BCL2, P53 and KI67 expression 
      were decreased in endometrial samples associated with females compared to their 
      male littermates. TUNEL staining revealed no association between fetal size or 
      sex, and apoptotic cell number. DISCUSSION: This study has highlighted dynamic 
      associations between fetal size, sex, and apoptosis and proliferation at the 
      porcine feto-maternal interface. Further studies should be performed to improve 
      the understanding of the mechanisms behind these findings.
CI  - Copyright (c) 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Stenhouse, Claire
AU  - Stenhouse C
AD  - Developmental Biology Division, The Roslin Institute and Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Midlothian, EH25 9RG, UK. Electronic 
      address: Claire.Stenhouse@roslin.ed.ac.uk.
FAU - Hogg, Charis O
AU  - Hogg CO
AD  - Developmental Biology Division, The Roslin Institute and Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Midlothian, EH25 9RG, UK.
FAU - Ashworth, Cheryl J
AU  - Ashworth CJ
AD  - Developmental Biology Division, The Roslin Institute and Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Midlothian, EH25 9RG, UK.
LA  - eng
GR  - BB/J004316/1/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180827
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Cell Proliferation/*physiology
MH  - Endometrium/*physiology
MH  - Female
MH  - Fetal Development/*physiology
MH  - Fetal Weight/*physiology
MH  - Placenta/*physiology
MH  - Pregnancy
MH  - Swine
PMC - PMC6215148
OTO - NOTNLM
OT  - Apoptosis
OT  - Endometrium
OT  - Intrauterine growth restriction (IUGR)
OT  - Placenta
OT  - Porcine
OT  - Sexual dimorphism
EDAT- 2018/10/15 06:00
MHDA- 2019/10/01 06:00
PMCR- 2018/10/01
CRDT- 2018/10/15 06:00
PHST- 2018/06/03 00:00 [received]
PHST- 2018/08/17 00:00 [revised]
PHST- 2018/08/24 00:00 [accepted]
PHST- 2018/10/15 06:00 [entrez]
PHST- 2018/10/15 06:00 [pubmed]
PHST- 2019/10/01 06:00 [medline]
PHST- 2018/10/01 00:00 [pmc-release]
AID - S0143-4004(18)30279-0 [pii]
AID - 10.1016/j.placenta.2018.08.006 [doi]
PST - ppublish
SO  - Placenta. 2018 Oct;70:15-24. doi: 10.1016/j.placenta.2018.08.006. Epub 2018 Aug 
      27.