PMID- 30317637
OWN - NLM
STAT- MEDLINE
DCOM- 20200413
LR  - 20200413
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 234
IP  - 6
DP  - 2019 Jun
TI  - Brain-derived neurotrophic factor alleviates the oxidative stress induced by 
      oxygen and glucose deprivation in an ex vivo brain slice model.
PG  - 9592-9604
LID - 10.1002/jcp.27646 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is considered as a putative therapeutic 
      agent against stroke. Since BDNF role on oxidative stress is uncertain, we have 
      studied this role in a rat brain slice ischemia model, which allows BDNF reaching 
      the neural parenchyma. Hippocampal and cerebral cortex slices were subjected to 
      oxygen and glucose deprivation (OGD) and then returned to normoxic conditions 
      (reperfusion-like, RL). OGD/RL increased a number of parameters mirroring 
      oxidative stress in the hippocampus that were reduced by the BDNF presence. BDNF 
      also reduced the OGD/RL-increased activity in a number of antioxidant enzymes in 
      the hippocampus but no effects were observed in the cerebral cortex. In general, 
      we conclude that alleviation of oxidative stress by BDNF in OGD/RL-exposed slices 
      relies on decreasing cPLA2 activity, rather than modifying antioxidant enzyme 
      activities. Moreover, a role for the oxidative stress in the differential 
      ischemic vulnerability of cerebral cortex and hippocampus is also supported.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Gonzalez-Rodriguez, Paloma
AU  - Gonzalez-Rodriguez P
AUID- ORCID: 0000-0002-3962-6858
AD  - Area de Biologia Celular, Instituto de Biomedicina, University of Leon, Leon, 
      Spain.
FAU - Ugidos, Irene F
AU  - Ugidos IF
AUID- ORCID: 0000-0002-3727-0544
AD  - Area de Biologia Celular, Instituto de Biomedicina, University of Leon, Leon, 
      Spain.
FAU - Perez-Rodriguez, Diego
AU  - Perez-Rodriguez D
AUID- ORCID: 0000-0003-4903-3914
AD  - Area de Biologia Celular, Instituto de Biomedicina, University of Leon, Leon, 
      Spain.
FAU - Anuncibay-Soto, Berta
AU  - Anuncibay-Soto B
AUID- ORCID: 0000-0001-7812-3754
AD  - Area de Biologia Celular, Instituto de Biomedicina, University of Leon, Leon, 
      Spain.
FAU - Santos-Galdiano, Maria
AU  - Santos-Galdiano M
AUID- ORCID: 0000-0002-9918-8319
AD  - Area de Biologia Celular, Instituto de Biomedicina, University of Leon, Leon, 
      Spain.
FAU - Font-Belmonte, Enrique
AU  - Font-Belmonte E
AUID- ORCID: 0000-0002-5695-7923
AD  - Area de Biologia Celular, Instituto de Biomedicina, University of Leon, Leon, 
      Spain.
FAU - Gonzalo-Orden, Jose Manuel
AU  - Gonzalo-Orden JM
AUID- ORCID: 0000-0002-9822-7566
AD  - Dpt. Medicina, Cirugia y Anatomia Veterinaria, University of Leon, Leon, Spain.
FAU - Fernandez-Lopez, Arsenio
AU  - Fernandez-Lopez A
AUID- ORCID: 0000-0001-5557-2741
AD  - Area de Biologia Celular, Instituto de Biomedicina, University of Leon, Leon, 
      Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181014
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Antioxidants)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 3.1.1.4 (Phospholipases A2)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Brain/*pathology
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Death/drug effects
MH  - Cell Survival/drug effects
MH  - Cytosol/enzymology
MH  - Glucose/*deficiency
MH  - Lipid Peroxidation/drug effects
MH  - Male
MH  - *Models, Biological
MH  - NADPH Oxidases/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Oxygen/*toxicity
MH  - Phospholipases A2/genetics/metabolism
MH  - Phosphorylation/drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - Reperfusion
MH  - Transcription, Genetic/drug effects
OTO - NOTNLM
OT  - BDNF
OT  - OGD
OT  - antioxidant defense system
OT  - cPLA2
OT  - oxidative stress
EDAT- 2018/10/15 06:00
MHDA- 2020/04/14 06:00
CRDT- 2018/10/15 06:00
PHST- 2018/02/08 00:00 [received]
PHST- 2018/09/02 00:00 [accepted]
PHST- 2018/10/15 06:00 [pubmed]
PHST- 2020/04/14 06:00 [medline]
PHST- 2018/10/15 06:00 [entrez]
AID - 10.1002/jcp.27646 [doi]
PST - ppublish
SO  - J Cell Physiol. 2019 Jun;234(6):9592-9604. doi: 10.1002/jcp.27646. Epub 2018 Oct 
      14.