PMID- 30317949
OWN - NLM
STAT- MEDLINE
DCOM- 20190124
LR  - 20211204
IS  - 1940-4344 (Electronic)
IS  - 1940-4344 (Linking)
VI  - 20
IP  - 8
DP  - 2018
TI  - Compound of Stout Camphor Medicinal Mushroom, Taiwanofungus camphoratus 
      (Agaricomycetes), Induces Protective Autophagy in SPCA-1 Cells through AMPK 
      Inhibition-Independent Blockade of the Akt/mTOR Pathway.
PG  - 727-738
LID - 10.1615/IntJMedMushrooms.2018026983 [doi]
AB  - Our previous study showed that By-1, a maleimide derivative isolated from 
      Taiwanofungus camphoratus, could induce reactive oxygen species-triggered 
      apoptosis and G2 cell cycle arrest through a caspase-dependent pathway and also 
      induced protective autophagy in human lung cancer SPCA-1 cells. Here, we further 
      examined the autophagy flux and detected related proteins by Western blot 
      analysis and fluorescence activated cell sorting, and we sought to find the exact 
      role and underlying pathway of autophagy in SPCA-1 cells. Our results showed that 
      By-1 treatment activated autophagy flux in SPCA-1 cells, which further confirmed 
      that autophagy was induced by By-1 treatment in our previous study. Autophagy 
      activator rapamycin restored cell death from By-1 treatment (21.32%) and verified 
      that autophagy played a protective role in By-l-treated cells. Meanwhile, By-1 
      treatment suppressed the Akt-mammalian target of rapamycin (mTOR) pathway and the 
      AMP-activated protein kinase (AMPK) pathway. Taken together, these findings 
      indicate that By-1 induced protective autophagy in SPCA-1 cells through AMPK 
      inhibition-independent blockade of the Akt/mTOR pathway.
FAU - Yang, Hairui
AU  - Yang H
AD  - National Engineering Research Center of Edible Fungi, Key Laboratory of Applied 
      Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key 
      Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, 
      Shanghai Academy of Agricultural Sciences, Shanghai, China; WuXi AppTec Co. Ltd., 
      Shanghai, China.
FAU - Zhang, Jinsong
AU  - Zhang J
AD  - National Engineering Research Center of Edible Fungi, Key Laboratory of Applied 
      Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key 
      Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, 
      Shanghai Academy of Agricultural Sciences, Shanghai, China.
FAU - Zhang, Henan
AU  - Zhang H
AD  - National Engineering Research Center of Edible Fungi, Key Laboratory of Applied 
      Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key 
      Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, 
      Shanghai Academy of Agricultural Sciences, Shanghai, China.
FAU - Yang, Yan
AU  - Yang Y
AD  - National Engineering Research Center of Edible Fungi, Key Laboratory of Applied 
      Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key 
      Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, 
      Shanghai Academy of Agricultural Sciences, Shanghai, China.
FAU - Liu, Yanfang
AU  - Liu Y
AD  - National Engineering Research Centre of Edible Fungi, Key Laboratory of Applied 
      Mycological Resources and Utilisation, Ministry of Agriculture, Institute of 
      Edible Fungi, Shanghai Academy of Agriculture Sciences, Shanghai, People's 
      Republic of China.
FAU - Sun, Wenbo
AU  - Sun W
AD  - Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, 
      Institute of Animal Science and Veterinary Medicine, Shandong Academy of 
      Agricultural Sciences, Jinan, China.
FAU - Wang, Wenhan
AU  - Wang W
AD  - National Engineering Research Center of Edible Fungi, Key Laboratory of Applied 
      Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key 
      Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, 
      Shanghai Academy of Agricultural Sciences, Shanghai, China.
FAU - Jia, Wei
AU  - Jia W
AD  - National Engineering Research Center of Edible Fungi, Key Laboratory of Applied 
      Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key 
      Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, 
      Shanghai Academy of Agricultural Sciences, Shanghai, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Med Mushrooms
JT  - International journal of medicinal mushrooms
JID - 100886202
RN  - 0 (Antineoplastic Agents)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.3 (AMP-Activated Protein Kinase Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinase Kinases
MH  - Antineoplastic Agents/chemistry/*pharmacology
MH  - Autophagy/*drug effects
MH  - Cell Line, Tumor
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Humans
MH  - Molecular Structure
MH  - Polyporales/*chemistry
MH  - Protein Kinases/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-akt/genetics/*metabolism
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
EDAT- 2018/10/16 06:00
MHDA- 2019/01/25 06:00
CRDT- 2018/10/16 06:00
PHST- 2018/10/16 06:00 [entrez]
PHST- 2018/10/16 06:00 [pubmed]
PHST- 2019/01/25 06:00 [medline]
AID - 66746bdc1bb430f2,75f5e4b12edfce93 [pii]
AID - 10.1615/IntJMedMushrooms.2018026983 [doi]
PST - ppublish
SO  - Int J Med Mushrooms. 2018;20(8):727-738. doi: 
      10.1615/IntJMedMushrooms.2018026983.