PMID- 30319363
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240401
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 12
DP  - 2018
TI  - Purinergic Profiling of Regulatory T-cells in Patients With Episodic Migraine.
PG  - 326
LID - 10.3389/fncel.2018.00326 [doi]
LID - 326
AB  - Objectives: Immune responses in migraine are poorly characterized, yet implicated 
      in the disease pathogenesis. This study was carried out to characterize 
      purinergic profiles of T-cells in patients with episodic migraine without aura 
      (MWoA) to provide mechanistic evidence for ATP and adenosine involvement in 
      modulation of immune regulation in migraine. Methods: Peripheral blood samples 
      were obtained from patients with migraine (n = 16) and age-matched control 
      subjects (n = 21). Subsets of T-cells were identified by flow cytometry based on 
      specific membrane markers. Results: Migraine patients showed reduced total T-cell 
      counts in the peripheral blood. Whereas the total number of CD3+CD4+, CD3+CD8+, 
      or regulatory T lymphocytes (Treg) was not changed, the proportion of Treg 
      CD45R0+CD62L- and CD45R0-CD62L- cells was increased. Interestingly, in migraine, 
      less Treg cells expressed CD39 and CD73 suggesting disrupted ATP breakdown to 
      adenosine. The negative correlations were observed between the duration of 
      migraine and the relative number of CD73+CD39- Tregs and total number of 
      CD73-positive CD45R0+CD62L+ Tregs. Conclusion: Obtained data indicate that T-cell 
      populations are altered in episodic migraine and suggest the involvement of Tregs 
      in the pathophysiology of this disorder. Reduced expression of CD39 and CD73 
      suggests promotion of ATP-dependent pro-inflammatory and reduction of 
      adenosine-mediated anti-inflammatory mechanisms in migraine.
FAU - Nurkhametova, Dilyara
AU  - Nurkhametova D
AD  - A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 
      Kuopio, Finland.
AD  - Laboratory of Neurobiology, Kazan Federal University, Kazan, Russia.
FAU - Kudryavtsev, Igor
AU  - Kudryavtsev I
AD  - Department of Immunology, Institute of Experimental Medicine, St. Petersburg, 
      Russia.
AD  - Department of Fundamental Medicine, Far Eastern Federal University, Vladivostok, 
      Russia.
FAU - Khayrutdinova, Olga
AU  - Khayrutdinova O
AD  - Department of Neurology and Rehabilitation, Kazan State Medical University, 
      Kazan, Russia.
FAU - Serebryakova, Maria
AU  - Serebryakova M
AD  - Department of Immunology, Institute of Experimental Medicine, St. Petersburg, 
      Russia.
FAU - Altunbaev, Rashid
AU  - Altunbaev R
AD  - Department of Neurology and Rehabilitation, Kazan State Medical University, 
      Kazan, Russia.
FAU - Malm, Tarja
AU  - Malm T
AD  - A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 
      Kuopio, Finland.
FAU - Giniatullin, Rashid
AU  - Giniatullin R
AD  - A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 
      Kuopio, Finland.
AD  - Laboratory of Neurobiology, Kazan Federal University, Kazan, Russia.
LA  - eng
PT  - Journal Article
DEP - 20180925
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC6167492
OTO - NOTNLM
OT  - ATP
OT  - adenosine
OT  - migraine
OT  - purinergic signaling
OT  - regulatory T cells
EDAT- 2018/10/16 06:00
MHDA- 2018/10/16 06:01
PMCR- 2018/01/01
CRDT- 2018/10/16 06:00
PHST- 2018/06/14 00:00 [received]
PHST- 2018/09/07 00:00 [accepted]
PHST- 2018/10/16 06:00 [entrez]
PHST- 2018/10/16 06:00 [pubmed]
PHST- 2018/10/16 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2018.00326 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2018 Sep 25;12:326. doi: 10.3389/fncel.2018.00326. 
      eCollection 2018.