PMID- 30321543
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 213
DP  - 2018 Nov 15
TI  - The protective effect of low-energy shock wave on testicular ischemia-reperfusion 
      injury is mediated by the PI3K/AKT/NRF2 pathway.
PG  - 142-148
LID - S0024-3205(18)30638-6 [pii]
LID - 10.1016/j.lfs.2018.10.021 [doi]
AB  - AIMS: Testicular ischemia-reperfusion (IR) injury is the primary 
      pathophysiological consequence of testicular torsion. Low-energy shock wave 
      (LESW) is an effective treatment for certain diseases. The present study 
      investigated whether LESW could improve on testicular IR injury in rats and 
      examined the involved mechanism. MAIN METHODS: Testicular reperfusion was induced 
      in rats after 1-h ischemia. The first LESW treatment was performed 30 min prior 
      to testicular reperfusion, and then every other day for another 3 applications. 
      LY294002 was applied to investigate the involved mechanism. Testicular 
      morphological changes and malonaldehyde (MDA) level were respectively assessed by 
      hematoxylin-eosin staining. Western blot and thiobarbituric acid method. Western 
      blot, real-time quantitative PCR and immunohistochemistry were performed to 
      assess the apoptosis, the activation of phosphatidylinositol-4,5-bisphosphate 
      3-kinase/protein kinase B (PI3K/AKT) pathway the nuclear factor erythroid 
      2-related factor 2 (NRF2) and vascular endothelial growth factor A (VEGF-A) level 
      in the testis of rats. KEY FINDINGS: LESW improved testicular IR injury in rats. 
      Moreover, LESW upregulated the phosphorylation levels of AKT and glycogen 
      synthase kinase 3beta (GSK-3beta). Also, it upregulated the levels of nuclear NRF2, 
      heme oxygenase 1 (HO-1) and NAD(P)H quinone dehydrogenase 1 (NQO-1) in these 
      rats. Nevertheless, LY294002 blocked these protective effects. LESW also 
      upregulated VEGF-A level in rats with testicular IR injury. SIGNIFICANCE: This 
      study demonstrated that LESW could ameliorate testicular IR injury in rats, which 
      might be attributed to the activation of PI3K/AKT/NRF2 pathway. These findings 
      suggested the potential of LESW in the treatment of testicular torsion.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Yu, Gang
AU  - Yu G
AD  - Department of Urology, Affiliated Haikou Hospital of Xiangya School of Medicine, 
      Central South University, Haikou 570208, Hainan Province, China; Haikou Center 
      for Medical Synchrotron Radiation Research, Haikou People's Hospital, Haikou 
      570208, Hainan Province, China.
FAU - Guan, Yupeng
AU  - Guan Y
AD  - Department of Urology, Affiliated Haikou Hospital of Xiangya School of Medicine, 
      Central South University, Haikou 570208, Hainan Province, China; Haikou Center 
      for Medical Synchrotron Radiation Research, Haikou People's Hospital, Haikou 
      570208, Hainan Province, China.
FAU - Liu, Lin
AU  - Liu L
AD  - Emergency and Critical Care Center, Shiyan People's Hospital, Shiyan 442000, 
      Hubei Province, China.
FAU - Xing, Jiansheng
AU  - Xing J
AD  - Department of Urology, Affiliated Haikou Hospital of Xiangya School of Medicine, 
      Central South University, Haikou 570208, Hainan Province, China.
FAU - Li, Jindong
AU  - Li J
AD  - Department of Urology, Affiliated Haikou Hospital of Xiangya School of Medicine, 
      Central South University, Haikou 570208, Hainan Province, China.
FAU - Cheng, Qing
AU  - Cheng Q
AD  - Department of Urology, Affiliated Haikou Hospital of Xiangya School of Medicine, 
      Central South University, Haikou 570208, Hainan Province, China.
FAU - Liu, Zhenxiang
AU  - Liu Z
AD  - Department of Urology, Affiliated Haikou Hospital of Xiangya School of Medicine, 
      Central South University, Haikou 570208, Hainan Province, China.
FAU - Bai, Zhiming
AU  - Bai Z
AD  - Department of Urology, Affiliated Haikou Hospital of Xiangya School of Medicine, 
      Central South University, Haikou 570208, Hainan Province, China; Haikou Center 
      for Medical Synchrotron Radiation Research, Haikou People's Hospital, Haikou 
      570208, Hainan Province, China. Electronic address: Drzmbai@163.com.
LA  - eng
PT  - Journal Article
DEP - 20181013
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Extracorporeal Shockwave Therapy/*methods
MH  - Glycogen Synthase Kinase 3 beta/metabolism
MH  - Male
MH  - NF-E2-Related Factor 2/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation/drug effects
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/metabolism/*prevention & control
MH  - Signal Transduction/drug effects
MH  - Sound
MH  - Spermatic Cord Torsion/*therapy
MH  - Testis/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
OTO - NOTNLM
OT  - Heme oxygenase 1
OT  - Low-energy shock wave
OT  - Nuclear factor erythroid 2-related factor 2
OT  - Protein kinase B
OT  - Testicular ischemia-reperfusion injury
OT  - Vascular endothelial growth factor A
EDAT- 2018/10/16 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/10/16 06:00
PHST- 2018/08/03 00:00 [received]
PHST- 2018/10/07 00:00 [revised]
PHST- 2018/10/11 00:00 [accepted]
PHST- 2018/10/16 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/10/16 06:00 [entrez]
AID - S0024-3205(18)30638-6 [pii]
AID - 10.1016/j.lfs.2018.10.021 [doi]
PST - ppublish
SO  - Life Sci. 2018 Nov 15;213:142-148. doi: 10.1016/j.lfs.2018.10.021. Epub 2018 Oct 
      13.