PMID- 30321544
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 214
DP  - 2018 Dec 1
TI  - Protective effects of SOD2 overexpression in human umbilical cord mesenchymal 
      stem cells on lung injury induced by acute paraquat poisoning in rats.
PG  - 11-21
LID - S0024-3205(18)30637-4 [pii]
LID - 10.1016/j.lfs.2018.10.020 [doi]
AB  - AIMS: To study the protective effects and mechanisms of human umbilical cord 
      mesenchymal stem cells (hUCMSCs) and overexpression of antioxidant gene SOD2 on 
      lung injury by establishing a rat model of paraquat (PQ)-induced lung injury. 
      MAIN METHODS: The hUCMSCs cell line overexpressed SOD2 was established. After 
      intraperitoneal injection of PQ solution (24 mg/kg) 3 h later, the different 
      groups of hUCMSCs cell lines were injected through the tail veins of rats. 
      Bronchoalveolar lavage fluid (BALF) was obtained to determine the protein level 
      of inflammatory cytokines. Lung tissues were collected to test the wet/dry weight 
      ratios (W/D), oxidative stress index and lung injury scores. Western blotting was 
      used to detect SOD1, SOD2, HO-1, Nrf2, NF-kappaBp65 subunit, and cleaved caspase-3. 
      KEY FINDINGS: After treatment with cells built on the basis of hUCMSCs, the 
      protein levels of TNF-alpha, IL-8, and ICAM-1 in BALF decreased, and meanwhile in 
      lung tissues, MDA content was reduced, GSH-Px activity was elevated, and lung W/D 
      ratio decreased. Additionally, protein expression of NF-kappaB p65 subunit and 
      activated caspase-3 in lung tissues was down-regulated, whereas expression of 
      SOD1, SOD2, HO-1, and Nrf-2 were up-regulated. The results of HE staining showed 
      that lung injury was significantly alleviated in the hUCMSC treated group. It is 
      noticeable that hUCMSCs and SOD2-overexpressed hUCMSCs effectively reduced 
      PQ-induced lung injury in rats, and moreover, hUCMSCs overexpressed SOD2 were 
      more effective compared with hUCMSCs only. SIGNIFICANCE: Evaluation of the 
      efficacy and analysis of mechanism in the treatment of PQ induced ALI by 
      appliance of SOD2-overexpressed hUCMSCs will provide the proof from bench to 
      bedside.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Zhang, Li-Chun
AU  - Zhang LC
AD  - Emergency Department of Shengjing Hospital of China Medical University, No. 36 
      Sanhao Street, Heping District, Shenyang, Liaoning Province 110004, China. 
      Electronic address: zhanglc@sj-hospital.org.
FAU - Wang, Yu
AU  - Wang Y
AD  - Emergency Department of Shengjing Hospital of China Medical University, No. 36 
      Sanhao Street, Heping District, Shenyang, Liaoning Province 110004, China.
FAU - Liu, Wei
AU  - Liu W
AD  - Emergency Department of Shengjing Hospital of China Medical University, No. 36 
      Sanhao Street, Heping District, Shenyang, Liaoning Province 110004, China.
FAU - Zhang, Xue-Min
AU  - Zhang XM
AD  - Eugenom Inc., Rm 310 No. 226 North Nanjing Street, Heping District, Shenyang, 
      Liaoning Province 110001, China.
FAU - Fan, Miao
AU  - Fan M
AD  - Eugenom Inc., Rm 310 No. 226 North Nanjing Street, Heping District, Shenyang, 
      Liaoning Province 110001, China.
FAU - Zhao, Min
AU  - Zhao M
AD  - Emergency Department of Shengjing Hospital of China Medical University, No. 36 
      Sanhao Street, Heping District, Shenyang, Liaoning Province 110004, China.
LA  - eng
PT  - Journal Article
DEP - 20181012
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Cytokines)
RN  - 0 (Transcription Factor RelA)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.15.1.1 (superoxide dismutase 2)
RN  - PLG39H7695 (Paraquat)
SB  - IM
MH  - Acute Lung Injury/*chemically induced/metabolism/pathology/*therapy
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Heme Oxygenase-1/metabolism
MH  - Humans
MH  - Infant, Newborn
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Oxidative Stress
MH  - Paraquat/*poisoning
MH  - Rats, Wistar
MH  - Superoxide Dismutase/genetics/*metabolism
MH  - Transcription Factor RelA/metabolism
MH  - Transgenes
OTO - NOTNLM
OT  - Acute paraquat poisoning
OT  - Human umbilical cord mesenchymal stem cells
OT  - Lung injury
OT  - Nuclear factor erythroid 2-related factor
OT  - Superoxide dismutase 2
EDAT- 2018/10/16 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/10/16 06:00
PHST- 2018/07/28 00:00 [received]
PHST- 2018/10/10 00:00 [revised]
PHST- 2018/10/11 00:00 [accepted]
PHST- 2018/10/16 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/10/16 06:00 [entrez]
AID - S0024-3205(18)30637-4 [pii]
AID - 10.1016/j.lfs.2018.10.020 [doi]
PST - ppublish
SO  - Life Sci. 2018 Dec 1;214:11-21. doi: 10.1016/j.lfs.2018.10.020. Epub 2018 Oct 12.