PMID- 30321547
OWN - NLM
STAT- MEDLINE
DCOM- 20200217
LR  - 20200217
IS  - 1520-6017 (Electronic)
IS  - 0022-3549 (Linking)
VI  - 108
IP  - 1
DP  - 2019 Jan
TI  - A Novel Approach via Surface Modification of Degradable Polymers With Adhesive 
      DOPA-IGF-1 for Neural Tissue Engineering.
PG  - 551-562
LID - S0022-3549(18)30606-3 [pii]
LID - 10.1016/j.xphs.2018.10.008 [doi]
AB  - The highly damaging state of spinal cord injuries has provided much inspiration 
      for the design of surface modification of the implants that can promote nerve 
      regeneration and functional reconstruction. DOPA-IGF-1, a new recombinant protein 
      designed in our previous study, exhibited strong binding affinity to titanium and 
      significantly enhanced the growth of NIH3T3 cells on the surface of titanium with 
      the same biological activity as IGF-1. In this article, surface modification of 
      poly(lactide-co-glycolide) (PLGA) films with recombinant DOPA-IGF-1 was performed 
      to promote the paracrine activity of human umbilical cord mesenchymal stem cells 
      (hUCMSCs) by secreting neurotrophic factors. DOPA-IGF-1 exhibited the strongest 
      binding ability to PLGA films than commercial IGF-1 and nonhydroxylated 
      YKYKY-IGF-1. In vitro cultures of hUCMSCs on the modified PLGA films showed that 
      DOPA-IGF-1@PLGA substrates significantly improved the proliferation, adhesion, 
      and neurotrophic factors secretion of hUCMSCs, especially for nerve growth 
      factor, as confirmed by qRT-PCR and western blot analysis. Subsequently, the 
      acquired neurotrophic factors secreted by the hUCMSCs cultured on the 
      DOPA-IGF-1@PLGA films obviously enhanced neurite outgrowth of PC12 cells. Taken 
      together, PLGA substrates with DOPA-IGF-1 immobilization is a promising platform 
      for neural tissue engineering via neurotrophic factors secretion from MSCs and 
      should be further tested in vivo.
CI  - Copyright (c) 2019. Published by Elsevier Inc.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Department of Orthopaedics, The Second Hospital, Jilin University, Changchun 
      130041, PR China; Key Laboratory of Polymer Ecomaterials, Changchun Institute of 
      Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
FAU - Wang, Zongliang
AU  - Wang Z
AD  - Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, 
      Chinese Academy of Sciences, Changchun 130022, PR China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, 
      Chinese Academy of Sciences, Changchun 130022, PR China.
FAU - Li, Linlong
AU  - Li L
AD  - Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, 
      Chinese Academy of Sciences, Changchun 130022, PR China.
FAU - Wu, Zhenxu
AU  - Wu Z
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun 130021, PR China.
FAU - Ito, Yoshihiro
AU  - Ito Y
AD  - Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter 
      Science 2-1 Hirosawa, Wako-shi, Saitama 351-0198 Japan.
FAU - Yang, Xiaoyu
AU  - Yang X
AD  - Department of Orthopaedics, The Second Hospital, Jilin University, Changchun 
      130041, PR China. Electronic address: yangxiaoyu88@sina.com.
FAU - Zhang, Peibiao
AU  - Zhang P
AD  - Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, 
      Chinese Academy of Sciences, Changchun 130022, PR China; Institute of Applied 
      Chemistry and Engineering, University of Science and Technology of China, Hefei 
      230026, PR China. Electronic address: zhangpb@ciac.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20181012
PL  - United States
TA  - J Pharm Sci
JT  - Journal of pharmaceutical sciences
JID - 2985195R
RN  - 0 (Adhesives)
RN  - 0 (Polymers)
RN  - 63-84-3 (Dihydroxyphenylalanine)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Adhesives/*chemistry
MH  - Animals
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Dihydroxyphenylalanine/*chemistry
MH  - Humans
MH  - Insulin-Like Growth Factor I/*chemistry/*pharmacology
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Neurites/*drug effects
MH  - PC12 Cells
MH  - Polymers/*chemistry
MH  - Rats
MH  - Tissue Engineering/methods
OTO - NOTNLM
OT  - DOPA-IGF-1
OT  - hUCMSCs
OT  - neural regeneration applications
OT  - neurotrophic factors
EDAT- 2018/10/16 06:00
MHDA- 2020/02/18 06:00
CRDT- 2018/10/16 06:00
PHST- 2018/07/18 00:00 [received]
PHST- 2018/09/26 00:00 [revised]
PHST- 2018/10/05 00:00 [accepted]
PHST- 2018/10/16 06:00 [pubmed]
PHST- 2020/02/18 06:00 [medline]
PHST- 2018/10/16 06:00 [entrez]
AID - S0022-3549(18)30606-3 [pii]
AID - 10.1016/j.xphs.2018.10.008 [doi]
PST - ppublish
SO  - J Pharm Sci. 2019 Jan;108(1):551-562. doi: 10.1016/j.xphs.2018.10.008. Epub 2018 
      Oct 12.