PMID- 30321818
OWN - NLM
STAT- MEDLINE
DCOM- 20190514
LR  - 20190514
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 65
DP  - 2018 Dec
TI  - Restoration of stemness-high tumor cell-mediated suppression of murine dendritic 
      cell activity and inhibition of tumor growth by low molecular weight oyster 
      polysaccharide.
PG  - 221-232
LID - S1567-5769(18)30809-9 [pii]
LID - 10.1016/j.intimp.2018.10.003 [doi]
AB  - Dendritic cells (DCs) play key regulatory roles in tumor immunity: increased 
      activity of DCs infiltrating tumor tissues leads to enhancement of tumor 
      immunity. Functions of DCs are also modulated by tumor cell-derived factors. 
      Here, we investigated the effects of low molecular weight oyster polysaccharide 
      (LMW-OPS) on differentiation and function of bone marrow-derived DCs (BMDCs) 
      exposed to a conditioned medium (CM) obtained from spheres of stemness-high 
      colorectal cancer cell lines CMT93 and CT26. The CM containing a detectable level 
      of TGF-beta1 was found to down-regulate the surface expression of major 
      histocompatibility complex class II of BMDCs and to inhibit the potency of BMDCs 
      to stimulate T cells. Those suppressions were partly restored and completely 
      restored by addition of anti-TGF-beta1 and LMW-OPS, respectively. Production of 
      IFN-gamma during allogeneic T cell responses was inhibited by the CM, whereas 
      production of TGF-beta1 was augmented by the CM. The IFN-gamma profile was also reversed 
      by addition of LMW-OPS. Nuclear translocation of beta-catenin, but not that of NF-kappaB 
      p65, was induced by TGF-beta1. NF-kappaB p65 nuclear translocation, but not beta-catenin 
      nuclear translocation, was induced by LMW-OPS. Intraperitoneal injection of 
      LMW-OPS significantly suppressed tumor growth in syngeneic tumor models using 
      CMT93 and CT26 sphere cells, whereas it had no inhibitory effect on the 
      proliferation of either cell line. The results demonstrated that LMW-OPS relieved 
      stemness-high tumor cell-mediated suppression of BMDC function and indicated the 
      in vivo anti-tumor activity of LMW-OPS in which re-stimulation of the activity of 
      DCs infiltrating tumor tissues is presumed to be involved.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Zhong, Ming
AU  - Zhong M
AD  - Institute of Tumor Pharmacology, Jining Medical College, Rizhao, China. 
      Electronic address: zhongming0410@163.com.
FAU - Zhong, Cheng
AU  - Zhong C
AD  - Division of Stem Cell Dynamics, Center for Stem Cell Biology and Regenerative 
      Medicine, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
FAU - Hu, Pei
AU  - Hu P
AD  - Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 
      China. Electronic address: hupeishtcm@126.com.
FAU - Cui, Wen
AU  - Cui W
AD  - Institute of Tumor Pharmacology, Jining Medical College, Rizhao, China.
FAU - Wang, Guanghui
AU  - Wang G
AD  - Institute of Tumor Pharmacology, Jining Medical College, Rizhao, China.
FAU - Gao, Huijei
AU  - Gao H
AD  - Institute of Tumor Pharmacology, Jining Medical College, Rizhao, China.
FAU - Liu, Chao
AU  - Liu C
AD  - Institute of Tumor Pharmacology, Jining Medical College, Rizhao, China.
FAU - Liu, Zhiqiang
AU  - Liu Z
AD  - Institute of Tumor Pharmacology, Jining Medical College, Rizhao, China.
FAU - Li, Zhihua
AU  - Li Z
AD  - Institute of Tumor Pharmacology, Jining Medical College, Rizhao, China.
FAU - Li, Chunxia
AU  - Li C
AD  - Institute of Tumor Pharmacology, Jining Medical College, Rizhao, China.
FAU - Gohda, Eiichi
AU  - Gohda E
AD  - Division of Pharmaceutical Sciences, Okayama University Graduate School of 
      Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
LA  - eng
PT  - Journal Article
DEP - 20181013
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Biological Products)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - Biological Products/*pharmacology
MH  - Bone Marrow Cells/*physiology
MH  - Cell Dedifferentiation
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Colorectal Neoplasms/*drug therapy/pathology
MH  - Dendritic Cells/*physiology/transplantation
MH  - Humans
MH  - Immune Tolerance
MH  - Immunotherapy/*methods
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Neoplastic Stem Cells/*physiology
MH  - Ostreidae/*chemistry
MH  - Polysaccharides/*pharmacology
OTO - NOTNLM
OT  - Colorectal cancer stem cells
OT  - Dendritic cells
OT  - NF-kappaB
OT  - Oyster polysaccharide
OT  - TGF-beta
EDAT- 2018/10/16 06:00
MHDA- 2019/05/15 06:00
CRDT- 2018/10/16 06:00
PHST- 2018/05/05 00:00 [received]
PHST- 2018/09/24 00:00 [revised]
PHST- 2018/10/03 00:00 [accepted]
PHST- 2018/10/16 06:00 [pubmed]
PHST- 2019/05/15 06:00 [medline]
PHST- 2018/10/16 06:00 [entrez]
AID - S1567-5769(18)30809-9 [pii]
AID - 10.1016/j.intimp.2018.10.003 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2018 Dec;65:221-232. doi: 10.1016/j.intimp.2018.10.003. Epub 
      2018 Oct 13.