PMID- 30322095
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20231004
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 19
IP  - 10
DP  - 2018 Oct 12
TI  - Histone Hyperacetylation as a Response to Global Brain Ischemia Associated with 
      Hyperhomocysteinemia in Rats.
LID - 10.3390/ijms19103147 [doi]
LID - 3147
AB  - Epigenetic regulations play an important role in both normal and pathological 
      conditions of an organism, and are influenced by various exogenous and endogenous 
      factors. Hyperhomocysteinemia (hHcy), as a risk factor for several pathological 
      conditions affecting the central nervous system, is supposed to alter the 
      epigenetic signature of the given tissue, which therefore worsens the subsequent 
      damage. To investigate the effect of hHcy in combination with 
      ischemia-reperfusion injury (IRI) and histone acetylation, we used the hHcy 
      animal model of global forebrain ischemia in rats. Cresyl violet staining showed 
      massive neural disintegration in the M1 (primary motor cortex) region as well as 
      in the CA1 (cornu ammonis 1) area of the hippocampus induced by IRI. Neural loss 
      was significantly higher in the group with induced hHcy. Moreover, 
      immunohistochemistry and Western blot analysis of the brain cortex showed 
      prominent changes in the acetylation of histones H3 and H4, at lysine 9 and 12, 
      respectively, as a result of IRI and induced hHcy. It seems that the differences 
      in histone acetylation patterns in the cortical region have a preferred role in 
      pathological processes induced by IRI associated with hHcy and could be 
      considered in therapeutic strategies.
FAU - Tothova, Barbara
AU  - Tothova B
AD  - Department of Molecular Medicine, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia. 
      tothova@jfmed.uniba.sk.
FAU - Kovalska, Maria
AU  - Kovalska M
AD  - Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia. kovalska@jfmed.uniba.sk.
FAU - Kalenska, Dagmar
AU  - Kalenska D
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia. kalenska@jfmed.uniba.sk.
FAU - Tomascova, Anna
AU  - Tomascova A
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia. benova111@uniba.sk.
FAU - Lehotsky, Jan
AU  - Lehotsky J
AUID- ORCID: 0000-0002-2408-5745
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia. lehotsky@jfmed.uniba.sk.
AD  - Department of Neuroscience, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia. 
      lehotsky@jfmed.uniba.sk.
LA  - eng
PT  - Journal Article
DEP - 20181012
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Histones)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Brain Ischemia/*complications/metabolism
MH  - Disease Models, Animal
MH  - Epigenesis, Genetic
MH  - Hippocampus/metabolism/*pathology
MH  - Histones/*metabolism
MH  - Hyperhomocysteinemia/*complications/metabolism
MH  - Lysine/metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
PMC - PMC6214033
OTO - NOTNLM
OT  - cortex
OT  - global forebrain ischemia
OT  - histone acetylation
OT  - homocysteine (Hcy)
OT  - ischemia-reperfusion injury (IRI)
OT  - rat
COIS- The authors declare no conflict of interest.
EDAT- 2018/10/17 06:00
MHDA- 2019/01/15 06:00
PMCR- 2018/10/01
CRDT- 2018/10/17 06:00
PHST- 2018/09/06 00:00 [received]
PHST- 2018/10/05 00:00 [revised]
PHST- 2018/10/10 00:00 [accepted]
PHST- 2018/10/17 06:00 [entrez]
PHST- 2018/10/17 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
PHST- 2018/10/01 00:00 [pmc-release]
AID - ijms19103147 [pii]
AID - ijms-19-03147 [pii]
AID - 10.3390/ijms19103147 [doi]
PST - epublish
SO  - Int J Mol Sci. 2018 Oct 12;19(10):3147. doi: 10.3390/ijms19103147.