PMID- 30322527
OWN - NLM
STAT- MEDLINE
DCOM- 20190130
LR  - 20190130
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 225
DP  - 2018 Nov
TI  - The immunomodulatory effect of antimicrobial peptide HPA3P restricts Brucella 
      abortus 544 infection in BALB/c mice.
PG  - 17-24
LID - S0378-1135(18)30979-9 [pii]
LID - 10.1016/j.vetmic.2018.09.005 [doi]
AB  - The discovery of antimicrobial peptides (AMPs) in recent years has been promising 
      for the treatment of multidrug resistant pathogenic microbes. Brucellosis is 
      still considered one of the most common zoonoses in the world. In this study, we 
      evaluated the effect HPA3P peptide in the bacterial uptake and intracellular 
      growth of Brucella abortus (B. abortus) 544 in murine macrophages RAW 264.7. 
      HPA3P was further utilized in a mouse model for infection and treatment. This 
      peptide did not show cytotoxicity or bactericidal effect to B. abortus. However, 
      it inhibited bacterial internalization at 0, 15 and 30 min incubation at two 
      different doses at 12 and 24 muM as well as reduced intracellular growth after 2, 
      24 and 48 h incubation. Mice treated with HPA3P demonstrated a significant 
      1.01-log reduction (P < 0.0001) and spleen weight reduction compared to the 
      nanocarrier control (P < 0.01). Significant increases in key cytokines 
      Interferon-gamma (IFN-gamma) and Tumor necrosis factor (TNF) at 3, 7 and 14 days 
      post-infection were observed in HPA3P treated mice similar to the antibiotic 
      control group with both compared to the nanocarrier control. Monocyte 
      chemoattractant protein-1 (MCP-1) was also heightened at 14 days post-infection. 
      Histopathological analysis also suggests reduced bacterial granuloma in the liver 
      and spleens of HPA3P treated group compared with the nanocarrier control group. 
      In this study, the modulation of crucial cytokines IFN-gamma and TNF might have led 
      to a considerable reduction in the proliferation of B. abortus in a mouse model 
      of brucellosis. Further investigation might be required to maximize the efficacy 
      of HPA3P treatment in murine brucellosis.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Arayan, Lauren Togonon
AU  - Arayan LT
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Jinju, 52828, Republic of Korea.
FAU - Kim, Hyeun Bum
AU  - Kim HB
AD  - Department of Animal Resources Science, Dankook University, Cheonan, 31116, 
      Republic of Korea.
FAU - Bernardo Reyes, Alisha Wehdnesday
AU  - Bernardo Reyes AW
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Jinju, 52828, Republic of Korea.
FAU - Xuan Huy, Ngoc Tran
AU  - Xuan Huy NT
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Jinju, 52828, Republic of Korea.
FAU - Hong, Il Hwa
AU  - Hong IH
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Jinju, 52828, Republic of Korea.
FAU - Lee, Kangseok
AU  - Lee K
AD  - Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of 
      Korea.
FAU - Yeom, Ji-Hyun
AU  - Yeom JH
AD  - Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of 
      Korea.
FAU - Park, Yoonkyung
AU  - Park Y
AD  - Department of Biomedical Science, Chosun University, Gwangju, 61452, Republic of 
      Korea.
FAU - Kim, Suk
AU  - Kim S
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Jinju, 52828, Republic of Korea. Electronic address: 
      kimsuk@gnu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20180913
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Cytokines)
RN  - 0 (Immunologic Factors)
RN  - 0 (Peptides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Brucella abortus/*drug effects/growth & development/immunology
MH  - Brucellosis/*immunology/microbiology
MH  - Cytokines/immunology
MH  - Disease Models, Animal
MH  - Immunologic Factors/administration & dosage/pharmacokinetics
MH  - Interferon-gamma/immunology
MH  - Liver/microbiology/pathology
MH  - Macrophages/*drug effects/immunology/microbiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Peptides/*administration & dosage/chemical synthesis/immunology/*pharmacology
MH  - Spleen/microbiology/pathology
MH  - Tumor Necrosis Factor-alpha/immunology
OTO - NOTNLM
OT  - Antimicrobial peptide
OT  - Brucella abortus
OT  - Cytokines
OT  - HPA3P
EDAT- 2018/10/17 06:00
MHDA- 2019/01/31 06:00
CRDT- 2018/10/17 06:00
PHST- 2018/08/22 00:00 [received]
PHST- 2018/09/11 00:00 [revised]
PHST- 2018/09/12 00:00 [accepted]
PHST- 2018/10/17 06:00 [entrez]
PHST- 2018/10/17 06:00 [pubmed]
PHST- 2019/01/31 06:00 [medline]
AID - S0378-1135(18)30979-9 [pii]
AID - 10.1016/j.vetmic.2018.09.005 [doi]
PST - ppublish
SO  - Vet Microbiol. 2018 Nov;225:17-24. doi: 10.1016/j.vetmic.2018.09.005. Epub 2018 
      Sep 13.