PMID- 30322778
OWN - NLM
STAT- MEDLINE
DCOM- 20190503
LR  - 20190503
IS  - 1878-4119 (Electronic)
IS  - 1010-5182 (Linking)
VI  - 46
IP  - 12
DP  - 2018 Dec
TI  - Cleft palate formation after palatal fusion occurs due to the rupture of 
      epithelial basement membranes.
PG  - 2027-2031
LID - S1010-5182(18)30470-0 [pii]
LID - 10.1016/j.jcms.2018.09.016 [doi]
AB  - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces cleft palate and 
      hydronephrosis in the mouse embryo. Cleft palate occurs due to failure in palatal 
      grow, but the underlying mechanisms are unclear. We investigated the mechanisms 
      of cleft palate development in TCDD-exposed mouse embryos. We administered olive 
      oil (control group) or TCDD diluted in olive oil (40 mug/kg) via gastric tubes to 
      pregnant mice on gestational day (GD) 12. Embryos of control and TCDD-exposed 
      groups were removed from pregnant mice on GD 14 and GD 15, respectively. One 
      mouse embryo from the control group had anteroposterior palatal fusion. Palatal 
      fusion was observed in three TCDD-exposed mouse embryos. Palates of TCDD-exposed 
      mice fused from the interior to the middle of the palates, while the palates were 
      separated in the posterior region. The middle of the embryonic palatal shelves in 
      TCDD-exposed animals was narrow and split at the fusional position. At this 
      position, palatal and blood cells were dispersed from the palatal tissue and the 
      epithelium was split, with a discontinuous basement membrane. The results suggest 
      that decreased intercellular adhesion or insufficient tissue strength of the 
      palatal shelves may be involved in the development of cleft palate following 
      palatal fusion.
CI  - Copyright (c) 2018 European Association for Cranio-Maxillo-Facial Surgery. 
      Published by Elsevier Ltd. All rights reserved.
FAU - Sakuma, Chisato
AU  - Sakuma C
AD  - Division of Research and Treatment for Oral and Maxillofacial Congenital 
      Anomalies, School of Dentistry, Aichi-Gakuin University, Japan.
FAU - Imura, Hideto
AU  - Imura H
AD  - Division of Research and Treatment for Oral and Maxillofacial Congenital 
      Anomalies, School of Dentistry, Aichi-Gakuin University, Japan. Electronic 
      address: h-imura@dpc.aichi-gakuin.ac.jp.
FAU - Yamada, Tomohiro
AU  - Yamada T
AD  - Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic 
      and Surgical Science, Faculty of Dental Science, Kyushu University, Japan.
FAU - Sugahara, Toshio
AU  - Sugahara T
AD  - Division of Research and Treatment for Oral and Maxillofacial Congenital 
      Anomalies, School of Dentistry, Aichi-Gakuin University, Japan.
FAU - Hirata, Azumi
AU  - Hirata A
AD  - Department of Anatomy and Cell Biology, Faculty of Medicine, Osaka Medical 
      College, Japan.
FAU - Ikeda, Yayoi
AU  - Ikeda Y
AD  - Department of Anatomy, School of Dentistry, Aichi-Gakuin University, Japan.
FAU - Natsume, Nagato
AU  - Natsume N
AD  - Division of Research and Treatment for Oral and Maxillofacial Congenital 
      Anomalies, School of Dentistry, Aichi-Gakuin University, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180920
PL  - Scotland
TA  - J Craniomaxillofac Surg
JT  - Journal of cranio-maxillo-facial surgery : official publication of the European 
      Association for Cranio-Maxillo-Facial Surgery
JID - 8704309
RN  - 0 (Polychlorinated Dibenzodioxins)
SB  - IM
MH  - Animals
MH  - Basement Membrane/*drug effects/*embryology
MH  - Cleft Palate/*chemically induced/*embryology
MH  - Female
MH  - Immunohistochemistry
MH  - Mice
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - Pregnancy
OTO - NOTNLM
OT  - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)
OT  - Basement membrane
OT  - Cell defect
OT  - Cleft palate
OT  - Post-fusional palatal split
EDAT- 2018/10/17 06:00
MHDA- 2019/05/06 06:00
CRDT- 2018/10/17 06:00
PHST- 2018/06/27 00:00 [received]
PHST- 2018/08/21 00:00 [revised]
PHST- 2018/09/12 00:00 [accepted]
PHST- 2018/10/17 06:00 [pubmed]
PHST- 2019/05/06 06:00 [medline]
PHST- 2018/10/17 06:00 [entrez]
AID - S1010-5182(18)30470-0 [pii]
AID - 10.1016/j.jcms.2018.09.016 [doi]
PST - ppublish
SO  - J Craniomaxillofac Surg. 2018 Dec;46(12):2027-2031. doi: 
      10.1016/j.jcms.2018.09.016. Epub 2018 Sep 20.