PMID- 30323762
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 9
DP  - 2018
TI  - Inhibition of WEE1 Suppresses the Tumor Growth in Laryngeal Squamous Cell 
      Carcinoma.
PG  - 1041
LID - 10.3389/fphar.2018.01041 [doi]
LID - 1041
AB  - WEE1 is a tyrosine kinase that regulates G2/M cell cycle checkpoint and 
      frequently overexpressed in various tumors. However, the expression and clinical 
      significance of WEE1 in human laryngeal squamous cell carcinoma (LSCC) are still 
      unknown. In this study, we found that WEE1 was highly expressed in LSCC tissues 
      compared with adjacent normal tissues. Importantly, overexpression of WEE1 was 
      correlated with T stages, lymph node metastasis, clinical stages and poor 
      prognosis of LSCC patients. Furthermore, inhibition of WEE1 by MK-1775 induced 
      cell growth inhibition, cell cycle arrest and apoptosis with the increased 
      intracellular reactive oxygen species (ROS) levels in LSCC cells. Pretreatment 
      with ROS scavenger N-acetyl-L-cysteine could reverse MK-1775-induced ROS 
      accumulation and cell apoptosis in LSCC cells. MK-1775 also inhibited the growth 
      of LSCC xenografts in nude mice. Altogether, these findings suggest that WEE1 is 
      a potential therapeutic target in LSCC, and inhibition of WEE1 is the prospective 
      strategy for LSCC therapy.
FAU - Yuan, Meng-Ling
AU  - Yuan ML
AD  - Department of Cell Biology - Institute of Biomedicine, National Engineering 
      Research Center of Genetic Medicine, Provincial Key Laboratory of Bioengineering 
      Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 
      China.
FAU - Li, Pei
AU  - Li P
AD  - Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated 
      Hospital, Sun Yat-sen University, Guangzhou, China.
FAU - Xing, Zi-Hao
AU  - Xing ZH
AD  - Department of Cell Biology - Institute of Biomedicine, National Engineering 
      Research Center of Genetic Medicine, Provincial Key Laboratory of Bioengineering 
      Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 
      China.
FAU - Di, Jin-Ming
AU  - Di JM
AD  - Department of Urology, The Third Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, China.
FAU - Liu, Hui
AU  - Liu H
AD  - Division of Pulmonary and Critical Care, Department of Internal Medicine, The 
      Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
FAU - Yang, An-Kui
AU  - Yang AK
AD  - Department of Head and Neck, Sun Yat-sen University Cancer Center, State Key 
      Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer 
      Medicine, Guangzhou, China.
FAU - Lin, Xi-Jun
AU  - Lin XJ
AD  - Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated 
      Hospital, Sun Yat-sen University, Guangzhou, China.
FAU - Jiang, Qi-Wei
AU  - Jiang QW
AD  - Department of Cell Biology - Institute of Biomedicine, National Engineering 
      Research Center of Genetic Medicine, Provincial Key Laboratory of Bioengineering 
      Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 
      China.
FAU - Yang, Yang
AU  - Yang Y
AD  - Department of Cell Biology - Institute of Biomedicine, National Engineering 
      Research Center of Genetic Medicine, Provincial Key Laboratory of Bioengineering 
      Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 
      China.
FAU - Huang, Jia-Rong
AU  - Huang JR
AD  - Department of Cell Biology - Institute of Biomedicine, National Engineering 
      Research Center of Genetic Medicine, Provincial Key Laboratory of Bioengineering 
      Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 
      China.
FAU - Wang, Kun
AU  - Wang K
AD  - Department of Cell Biology - Institute of Biomedicine, National Engineering 
      Research Center of Genetic Medicine, Provincial Key Laboratory of Bioengineering 
      Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 
      China.
FAU - Wei, Meng-Ning
AU  - Wei MN
AD  - Department of Cell Biology - Institute of Biomedicine, National Engineering 
      Research Center of Genetic Medicine, Provincial Key Laboratory of Bioengineering 
      Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 
      China.
FAU - Li, Yao
AU  - Li Y
AD  - Department of Cell Biology - Institute of Biomedicine, National Engineering 
      Research Center of Genetic Medicine, Provincial Key Laboratory of Bioengineering 
      Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 
      China.
FAU - Ye, Jin
AU  - Ye J
AD  - Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated 
      Hospital, Sun Yat-sen University, Guangzhou, China.
FAU - Shi, Zhi
AU  - Shi Z
AD  - Department of Cell Biology - Institute of Biomedicine, National Engineering 
      Research Center of Genetic Medicine, Provincial Key Laboratory of Bioengineering 
      Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20180928
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC6172786
OTO - NOTNLM
OT  - MK-1775
OT  - WEE1
OT  - apoptosis
OT  - cell cycle
OT  - laryngeal squamous cell carcinoma
OT  - reactive oxygen species
EDAT- 2018/10/17 06:00
MHDA- 2018/10/17 06:01
PMCR- 2018/09/28
CRDT- 2018/10/17 06:00
PHST- 2018/06/12 00:00 [received]
PHST- 2018/08/27 00:00 [accepted]
PHST- 2018/10/17 06:00 [entrez]
PHST- 2018/10/17 06:00 [pubmed]
PHST- 2018/10/17 06:01 [medline]
PHST- 2018/09/28 00:00 [pmc-release]
AID - 10.3389/fphar.2018.01041 [doi]
PST - epublish
SO  - Front Pharmacol. 2018 Sep 28;9:1041. doi: 10.3389/fphar.2018.01041. eCollection 
      2018.