PMID- 30324095
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220321
IS  - 2253-2447 (Print)
IS  - 2253-2447 (Electronic)
IS  - 2253-2447 (Linking)
VI  - 10
DP  - 2018
TI  - Caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer.
PG  - 135-144
LID - 10.2147/RRU.S173377 [doi]
AB  - BACKGROUND: Caveolin (Cav)-1 and Cav-2 are cell membrane proteins, which are 
      structural proteins of caveolae and are reported to be positive regulators of 
      cell survival and metastasis in prostate cancer (PC). In a previous study, we 
      reported that elevated levels of Cav-1 and Cav-2 were significantly associated 
      with PC progression. However, their functions in PC have not yet been clarified. 
      In this study, we examined the function of Cav-1 and Cav-2 in PC cell 
      invasiveness and motility. MATERIALS AND METHODS: We introduced Cav-1- and 
      Cav-2-specific small interfering into PC3 cells to knock-down (KD) both 
      molecules. We also performed cell proliferation assay, wound healing assay, 
      migration assay, and invasion assay using PC3 cells and compared the results 
      between Cav-1-KD, Cav-2-KD, and negative control PC3 cells. In addition, we 
      performed real-time quantitative PCR (RT-qPCR) and RT2 Profiler PCR Array 
      analysis to identify factors influencing migration. RESULTS: We observed no 
      significant difference in the proliferative and invasive activities of Cav-1-KD 
      and Cav-2-KD PC3 cells; however, the cell motility was significantly decreased 
      compared with negative control PC3 cells. RT-qPCR revealed that the expression of 
      vimentin and N-cadherin was downregulated in Cav-1-KD PC3 cells. In addition, PCR 
      array revealed a decreased expression of MGAT5, MMP13, and MYCL in Cav-1-KD PC3 
      and ETV4, FGFR4, and SRC in Cav-2-KD PC3. CONCLUSION: Cav-1 and Cav-2 may 
      positively contribute to the upregulation of castration-resistant PC cell 
      migration. Cav-induced regulation of several molecules including vimentin, 
      N-cadherin, MGAT5, MMP13, MYCL, ETV4, FGFR4, and SRC may have an important role 
      in PC3 cell motility. However, further examination will be required.
FAU - Kamibeppu, Toyoharu
AU  - Kamibeppu T
AD  - Department of Urology, Faculty of Medicine, University of Miyazaki, Kiyotake, 
      Japan, syoichiro_mukai@med.miyazaki-u.ac.jp.
FAU - Yamasaki, Koji
AU  - Yamasaki K
AD  - Department of Urology, Faculty of Medicine, University of Miyazaki, Kiyotake, 
      Japan, syoichiro_mukai@med.miyazaki-u.ac.jp.
FAU - Nakahara, Kozue
AU  - Nakahara K
AD  - Department of Urology, Faculty of Medicine, University of Miyazaki, Kiyotake, 
      Japan, syoichiro_mukai@med.miyazaki-u.ac.jp.
FAU - Nagai, Takahiro
AU  - Nagai T
AD  - Department of Urology, Faculty of Medicine, University of Miyazaki, Kiyotake, 
      Japan, syoichiro_mukai@med.miyazaki-u.ac.jp.
FAU - Terada, Naoki
AU  - Terada N
AD  - Department of Urology, Faculty of Medicine, University of Miyazaki, Kiyotake, 
      Japan, syoichiro_mukai@med.miyazaki-u.ac.jp.
FAU - Tsukino, Hiromasa
AU  - Tsukino H
AD  - Department of Urology, Faculty of Medicine, University of Miyazaki, Kiyotake, 
      Japan, syoichiro_mukai@med.miyazaki-u.ac.jp.
FAU - Mukai, Shoichiro
AU  - Mukai S
AD  - Department of Urology, Faculty of Medicine, University of Miyazaki, Kiyotake, 
      Japan, syoichiro_mukai@med.miyazaki-u.ac.jp.
FAU - Kamoto, Toshiyuki
AU  - Kamoto T
AD  - Department of Urology, Faculty of Medicine, University of Miyazaki, Kiyotake, 
      Japan, syoichiro_mukai@med.miyazaki-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20181003
PL  - England
TA  - Res Rep Urol
JT  - Research and reports in urology
JID - 101576971
PMC - PMC6174915
OTO - NOTNLM
OT  - CRPC
OT  - caveolin-1
OT  - caveolin-2
OT  - cell motility
COIS- Disclosure The authors report no conflicts of interest in this work.
EDAT- 2018/10/17 06:00
MHDA- 2018/10/17 06:01
PMCR- 2018/10/03
CRDT- 2018/10/17 06:00
PHST- 2018/10/17 06:00 [entrez]
PHST- 2018/10/17 06:00 [pubmed]
PHST- 2018/10/17 06:01 [medline]
PHST- 2018/10/03 00:00 [pmc-release]
AID - rru-10-135 [pii]
AID - 10.2147/RRU.S173377 [doi]
PST - epublish
SO  - Res Rep Urol. 2018 Oct 3;10:135-144. doi: 10.2147/RRU.S173377. eCollection 2018.