PMID- 303245
OWN - NLM
STAT- MEDLINE
DCOM- 19771229
LR  - 20190501
IS  - 0021-9746 (Print)
IS  - 1472-4146 (Electronic)
IS  - 0021-9746 (Linking)
VI  - 30
IP  - 9
DP  - 1977 Sep
TI  - Evaluation of a standardised assay for the measurement of antibodies to 
      double-stranded (native) DNA.
PG  - 827-30
AB  - A standardised commercially available radioimmunoassay kit for the detection of 
      antibodies to native DNA (N-DNA) has been evaluated in clinical practice. This 
      test system is shown to be a reliably reproducible method of detecting these 
      antibodies. In addition, evaluation of the purity of the radiolabelled test 
      antigen in this assay has shown it to be almost entirely double stranded (native) 
      DNA with virtually no contamination with single stranded (denatured) DNA, and 
      with few areas of single stranded breaks or ends in the duplex molecule. The 
      inclusion of known standards and precise characterisation of the DNA has 
      partially overcome variability in results and provides for interlaboratory 
      standardisation which is lacking in the techniques used at present.
FAU - Davis, P
AU  - Davis P
FAU - Burrington, M
AU  - Burrington M
FAU - Morgan, A R
AU  - Morgan AR
FAU - Saetre, A
AU  - Saetre A
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Clin Pathol
JT  - Journal of clinical pathology
JID - 0376601
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (DNA, Single-Stranded)
RN  - 9007-49-2 (DNA)
RN  - EN464416SI (Ethidium)
SB  - IM
MH  - Antibodies, Antinuclear/*analysis
MH  - DNA/*immunology
MH  - DNA, Single-Stranded/immunology
MH  - Ethidium
MH  - Evaluation Studies as Topic
MH  - Humans
MH  - Radioimmunoassay/*methods/standards
PMC - PMC476568
EDAT- 1977/09/01 00:00
MHDA- 1977/09/01 00:01
PMCR- 1977/09/01
CRDT- 1977/09/01 00:00
PHST- 1977/09/01 00:00 [pubmed]
PHST- 1977/09/01 00:01 [medline]
PHST- 1977/09/01 00:00 [entrez]
PHST- 1977/09/01 00:00 [pmc-release]
AID - 10.1136/jcp.30.9.827 [doi]
PST - ppublish
SO  - J Clin Pathol. 1977 Sep;30(9):827-30. doi: 10.1136/jcp.30.9.827.