PMID- 30325683
OWN - NLM
STAT- MEDLINE
DCOM- 20190624
LR  - 20190624
IS  - 1532-4311 (Electronic)
IS  - 0882-0139 (Linking)
VI  - 48
IP  - 3
DP  - 2019 Apr
TI  - Evaluation and characteristics of immunological adjuvant activity of purified 
      fraction of Albizia julibrissin saponins.
PG  - 283-302
LID - 10.1080/08820139.2018.1523923 [doi]
AB  - PURPOSE: The purified fraction of Albizia julibrissin saponins (AJSAF) was 
      evaluated and characterized for the adjuvant activity on porcine reproductive and 
      respiratory syndrome virus (PRRSV) vaccine. METHODS: The effects of AJSAF on 
      serum PRRSV N protein-specific antibody titers, splenocyte proliferation, natural 
      killer (NK) cell activity, mRNA expression of cytokines and transcription 
      factors, secretion of cytokines, T cells response in splenocytes, as well as 
      delayed type hypersensitivity (DTH) in the mice immunized PRRSV vaccine were 
      determined by ELISA, MTT assay, flow cytometry and quantitative real-time PCR 
      (qRT-PCR). RESULTS: AJSAF not only significantly enhanced the serum PRRSV N 
      protein-specific IgG, IgG1, IgG2a and IgG2b antibody titers in the mice immunized 
      with PRRSV CH-1R modified live vaccine (CH-1R MLV), inactivated vaccine (CH-1R 
      IAV), and highly pathogenic JXA1-R modified live vaccine (JXA1-R MLV), but 
      promoted the concanavalin A (Con A)-, lipopolysaccharide (LPS)- and PRRSV N 
      protein-stimulated splenocyte proliferation, the activities of NK cells and 
      delayed type hypersensitivity (DTH) in the mice immunized CH-1R MLV. AJSAF also 
      remarkably induced the production of both Th1 (IFN-gamma) and Th2 (IL-10) cytokines, 
      and up-regulated the mRNA expression levels of Th1 cytokines (IFN-gamma and IL-2) and 
      transcription factors (T-bet and STAT4) as well as Th2 cytokines (IL-4 and IL-10) 
      and transcription factors (GATA-3 and STAT6) in splenocytes from the CH-1R 
      MLV-immunized mice. Furthermore, AJSAF markedly increased the frequencies of 
      PRRSV N protein-specific Th1 (INF-gamma(+) and IL-2(+)) and Th2 (IL-4+ and IL-10(+)) 
      CD4 T cells as well as Tc1 (INF-gamma(+) and IL-2(+)) and Tc2 (IL-4(+) and IL-10(+)) 
      CD8 T cells in splenocytes from the CH-1R MLV-immunized mice. CONCLUSIONS: Our 
      results demonstrated that AJSAF had a potential to enhance and improve immune 
      responses and elicit both Th1/Th2 and Tc1/Tc2 response to PRRSV vaccine, and that 
      AJSAF would be a promising adjuvant candidate for PRRSV vaccine.
FAU - Zhu, Binnian
AU  - Zhu B
AD  - a College of Animal Sciences , Zhejiang University , Hangzhou , Zhejiang China.
FAU - He, Tianyu
AU  - He T
AD  - b College of Medicine , Zhejiang University , Hangzhou , Zhejiang China.
FAU - Gao, Xiangyun
AU  - Gao X
AD  - a College of Animal Sciences , Zhejiang University , Hangzhou , Zhejiang China.
FAU - Shi, Minghua
AU  - Shi M
AD  - a College of Animal Sciences , Zhejiang University , Hangzhou , Zhejiang China.
FAU - Sun, Hongxiang
AU  - Sun H
AD  - a College of Animal Sciences , Zhejiang University , Hangzhou , Zhejiang China.
LA  - eng
PT  - Journal Article
DEP - 20181016
PL  - England
TA  - Immunol Invest
JT  - Immunological investigations
JID - 8504629
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Saponins)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage/isolation & purification
MH  - Albizzia/*chemistry
MH  - Animals
MH  - Antibodies, Viral
MH  - Disease Models, Animal
MH  - Female
MH  - Immunogenicity, Vaccine
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred ICR
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - Saponins/*administration & dosage/immunology/isolation & purification
MH  - Swine
MH  - Treatment Outcome
MH  - Vaccination/methods
MH  - Vaccines, Attenuated/administration & dosage
MH  - Viral Vaccines/*administration & dosage/immunology
OTO - NOTNLM
OT  - saponin
OT  - Adjuvant
OT  - PRRSV vaccine
OT  - T cells response
OT  - immune response
OT  - purified active fraction
EDAT- 2018/10/17 06:00
MHDA- 2019/06/25 06:00
CRDT- 2018/10/17 06:00
PHST- 2018/10/17 06:00 [pubmed]
PHST- 2019/06/25 06:00 [medline]
PHST- 2018/10/17 06:00 [entrez]
AID - 10.1080/08820139.2018.1523923 [doi]
PST - ppublish
SO  - Immunol Invest. 2019 Apr;48(3):283-302. doi: 10.1080/08820139.2018.1523923. Epub 
      2018 Oct 16.