PMID- 30326207
OWN - NLM
STAT- MEDLINE
DCOM- 20190325
LR  - 20190325
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Linking)
VI  - 178
DP  - 2019 Jan
TI  - Pre-ischemic enriched environment increases retinal resilience to acute ischemic 
      damage in adult rats.
PG  - 198-211
LID - S0014-4835(18)30383-X [pii]
LID - 10.1016/j.exer.2018.10.007 [doi]
AB  - Retinal ischemia is a condition associated with several degenerative diseases 
      leading to visual impairment and blindness worldwide. Currently, there is no 
      highly effective therapy for ischemic retinopathies. This study was designed to 
      determine possible benefits of pre-exposure to enriched environment against 
      retinal damage induced by acute ischemia. For this purpose, adult male Wistar 
      rats were randomly assigned to a pre-ischemic standard environment or a 
      pre-ischemic enriched environment for 3 weeks, followed by unilateral ischemia 
      induced by increasing intraocular pressure above 120 mm Hg for 40 min and 
      reperfusion for 1 or 2 weeks in standard environment. Animals were subjected to 
      electroretinography and histological analysis. Pre-ischemic enriched environment 
      afforded significant functional protection in eyes exposed to 
      ischemia/reperfusion injury. A marked reduction in retinal layer thickness, 
      reduced synaptophysin-immunoreactivity and retinal ganglion cell (RGC) number, 
      and increased microglia/macrophage reactivity were observed in ischemic retinas 
      from animals submitted to pre-ischemic standard environment, which were prevented 
      by pre-ischemic enriched environment. A deficit in anterograde transport from the 
      retina to the superior colliculus and the lateral geniculate nucleus was observed 
      in animals exposed to pre-ischemic standard environment, which was lower in 
      animals previously exposed to enriched environment. The exposure to enriched 
      environment before ischemia increased retinal brain derived neurotrophic factor 
      (BDNF) protein levels in ischemic retinas and the administration of ANA-12 (a 
      TrkB antagonist) abolished the protective effect of enriched environment on 
      retinal function and retinal ganglion cell number. These results indicate that 
      pre-ischemic enriched environment increases retinal resilience to acute ischemic 
      damage, possibly through a BDNF/TrkB mediated pathway.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Gonzalez Fleitas, Maria F
AU  - Gonzalez Fleitas MF
AD  - Laboratory of Retinal Neurochemistry and Experimental Ophthalmology, Department 
      of Human Biochemistry, School of Medicine, University of Buenos Aires/CEFyBO, 
      CONICET, Buenos Aires, Argentina.
FAU - Aranda, Marcos L
AU  - Aranda ML
AD  - Laboratory of Retinal Neurochemistry and Experimental Ophthalmology, Department 
      of Human Biochemistry, School of Medicine, University of Buenos Aires/CEFyBO, 
      CONICET, Buenos Aires, Argentina.
FAU - Dieguez, Hernan H
AU  - Dieguez HH
AD  - Laboratory of Retinal Neurochemistry and Experimental Ophthalmology, Department 
      of Human Biochemistry, School of Medicine, University of Buenos Aires/CEFyBO, 
      CONICET, Buenos Aires, Argentina.
FAU - Devouassoux, Julian D
AU  - Devouassoux JD
AD  - Laboratory of Retinal Neurochemistry and Experimental Ophthalmology, Department 
      of Human Biochemistry, School of Medicine, University of Buenos Aires/CEFyBO, 
      CONICET, Buenos Aires, Argentina.
FAU - Chianelli, Monica S
AU  - Chianelli MS
AD  - Laboratory of Retinal Neurochemistry and Experimental Ophthalmology, Department 
      of Human Biochemistry, School of Medicine, University of Buenos Aires/CEFyBO, 
      CONICET, Buenos Aires, Argentina.
FAU - Dorfman, Damian
AU  - Dorfman D
AD  - Laboratory of Retinal Neurochemistry and Experimental Ophthalmology, Department 
      of Human Biochemistry, School of Medicine, University of Buenos Aires/CEFyBO, 
      CONICET, Buenos Aires, Argentina.
FAU - Rosenstein, Ruth E
AU  - Rosenstein RE
AD  - Laboratory of Retinal Neurochemistry and Experimental Ophthalmology, Department 
      of Human Biochemistry, School of Medicine, University of Buenos Aires/CEFyBO, 
      CONICET, Buenos Aires, Argentina. Electronic address: ruthr@fmed.uba.ar.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181013
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (ANA 12 compound)
RN  - 0 (Azepines)
RN  - 0 (Benzamides)
RN  - 0 (Biomarkers)
RN  - 0 (Eye Proteins)
RN  - 9012-63-9 (Cholera Toxin)
SB  - IM
MH  - *Adaptation, Physiological
MH  - Animal Husbandry/*methods
MH  - Animals
MH  - Azepines/pharmacology
MH  - Benzamides/pharmacology
MH  - Biomarkers/metabolism
MH  - Blotting, Western
MH  - Cholera Toxin/metabolism
MH  - Electroretinography
MH  - *Environment
MH  - Eye Proteins/metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/metabolism/physiopathology/*prevention & control
MH  - Retina/physiopathology
MH  - Retinal Diseases/metabolism/physiopathology/*prevention & control
MH  - Retinal Ganglion Cells/cytology
MH  - Retinal Vessels/physiopathology
OTO - NOTNLM
OT  - Brain derived neurotrophic factor
OT  - Ischemia
OT  - Pre-ischemic enriched environment
OT  - Retina
EDAT- 2018/10/17 06:00
MHDA- 2019/03/26 06:00
CRDT- 2018/10/17 06:00
PHST- 2018/05/17 00:00 [received]
PHST- 2018/10/02 00:00 [revised]
PHST- 2018/10/12 00:00 [accepted]
PHST- 2018/10/17 06:00 [pubmed]
PHST- 2019/03/26 06:00 [medline]
PHST- 2018/10/17 06:00 [entrez]
AID - S0014-4835(18)30383-X [pii]
AID - 10.1016/j.exer.2018.10.007 [doi]
PST - ppublish
SO  - Exp Eye Res. 2019 Jan;178:198-211. doi: 10.1016/j.exer.2018.10.007. Epub 2018 Oct 
      13.