PMID- 30328567
OWN - NLM
STAT- MEDLINE
DCOM- 20201013
LR  - 20211204
IS  - 1993-0402 (Electronic)
IS  - 1672-0415 (Linking)
VI  - 26
IP  - 5
DP  - 2020 May
TI  - Composition of Ophiopogon Polysaccharide, Notoginseng Total Saponins and Rhizoma 
      Coptidis Alkaloids Inhibits the Myocardial Apoptosis on Diabetic Atherosclerosis 
      Rabbit.
PG  - 353-360
LID - 10.1007/s11655-018-3014-2 [doi]
AB  - OBJECTIVE: To investigate the effects of Composition of Ophiopogon 
      polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) 
      on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty 
      male New Zealand white rabbits were randomly divided into 6 groups [control 
      group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 
      mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a 
      completely random method, 10 in each group. DA model was established by 
      intravenously injected alloxan combined with high-fat diet and abdominal aortic 
      balloon injury. After mediation for 10 weeks, fasting blood glucose (FBG), 
      glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine 
      (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum 
      were measured by enzyme linked immunosorbent assay (ELISA) method; the expression 
      of receptor of AGEs (RAGE) in myocardial tissue were observed by 
      immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, 
      B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured 
      by Western blotting. The myocardial apoptosis was detected by TdT-mediated 
      dUTPnick-end labeling (TUNEL) method, and apoptosis index (AI) was calculated. 
      RESULTS: Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and 
      the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as 
      apoptosis index (AI) were significantly increased and bcl-2 protein was 
      significantly decreased in the model group (P<0.01). Compared with the model 
      group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline 
      in CONR high- and medium-dose groups (P<0.01). FBG and GHB showed a significant 
      decline in CONR low-dose group (P<0.01). Compared with the model group, the 
      expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well 
      as AI were significantly decreased and bcl-2 protein was significantly 
      up-regulated in all treatment groups (P<0.01); high-dose CONR had the most 
      significant effect on abovementioned indices compared with other treatment groups 
      (P<0.01). Middle-dose CONR had better effect on serum AGEs compared with the 
      low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better 
      effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis 
      compared with the CONR low-dose group (P<0.01). CONCLUSION: CONR may effectively 
      inhibit myocardial apoptosis on DA rabbits by intervening AGEs-RAGE and JNK, 
      caspase-3, and bcl-2 protein expressions.
FAU - Jin, Zhao-Hui
AU  - Jin ZH
AD  - Geriatric Research Center, Xiyuan Hospital, China Academy of Chinese Medicine 
      Sciences, Beijing, 100091, China.
FAU - Gao, Pu
AU  - Gao P
AD  - Geriatric Research Center, Xiyuan Hospital, China Academy of Chinese Medicine 
      Sciences, Beijing, 100091, China. gaopubj2011@126.com.
FAU - Liu, Zheng-Tang
AU  - Liu ZT
AD  - Geriatric Research Center, Xiyuan Hospital, China Academy of Chinese Medicine 
      Sciences, Beijing, 100091, China.
FAU - Jin, Bing
AU  - Jin B
AD  - Geriatric Research Center, Xiyuan Hospital, China Academy of Chinese Medicine 
      Sciences, Beijing, 100091, China.
FAU - Song, Guang-Yi
AU  - Song GY
AD  - Liaoning Basic Medical Research Institute, Shenyang, 110005, China.
FAU - Xiang, Tian-Yuan
AU  - Xiang TY
AD  - Geriatric Research Center, Xiyuan Hospital, China Academy of Chinese Medicine 
      Sciences, Beijing, 100091, China.
LA  - eng
PT  - Journal Article
DEP - 20181017
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 0 (Alkaloids)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Polysaccharides)
RN  - 0 (Saponins)
RN  - CXS4LJR7EL (Coptidis rhizoma extract)
SB  - IM
MH  - Alkaloids/pharmacology
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Atherosclerosis
MH  - Coptis chinensis
MH  - Diabetes Mellitus, Experimental
MH  - Diabetic Angiopathies/*drug therapy
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Heart/drug effects
MH  - Male
MH  - Ophiopogon/*chemistry
MH  - Panax notoginseng/chemistry
MH  - Polysaccharides/*pharmacology
MH  - Rabbits
MH  - Saponins/*pharmacology
OTO - NOTNLM
OT  - Rhizoma Coptidis alkaloids
OT  - advanced glycation end products
OT  - diabetic atherosclerosis
OT  - myocardial apoptosis
OT  - notoginseng total saponins
OT  - ophiopogon polysaccharide
OT  - p-C-N-Jun terminal kinase
EDAT- 2018/10/18 06:00
MHDA- 2020/10/21 06:00
CRDT- 2018/10/18 06:00
PHST- 2018/05/19 00:00 [accepted]
PHST- 2018/10/18 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2018/10/18 06:00 [entrez]
AID - 10.1007/s11655-018-3014-2 [pii]
AID - 10.1007/s11655-018-3014-2 [doi]
PST - ppublish
SO  - Chin J Integr Med. 2020 May;26(5):353-360. doi: 10.1007/s11655-018-3014-2. Epub 
      2018 Oct 17.