PMID- 30332393
OWN - NLM
STAT- MEDLINE
DCOM- 20190621
LR  - 20190621
IS  - 2191-0286 (Electronic)
IS  - 0792-6855 (Linking)
VI  - 30
IP  - 1
DP  - 2018 Dec 19
TI  - Dynamics of inflammatory reaction and oxidative stress across maternal serum, 
      placenta and amniotic fluid in laboratory rats and the role played by genistein 
      aglycone.
PG  - 37-45
LID - 10.1515/jbcpp-2018-0070 [doi]
AB  - Background Genistein was reported to adversely influence fetal development 
      although this is yet to be fully understood as a mechanism. Methods In this 
      study, pregnant rats were divided into control (Cont.) and genistein force-fed 
      (2-mg/kg and 4-mg/kg) groups. Each group was divided further into five subgroups: 
      GD-0, GD-6, GD-13, GD-18, and GD-20 based on the terminal gestational day (GD). 
      On the respective terminal GD, the rats were sacrificed and blood samples and 
      amniotic fluid were carefully collected and separated and placenta homogenates 
      were prepared. These samples were evaluated for oxidative stress and inflammatory 
      reaction. The weights of embryonic implant and placenta tissue were also 
      recorded. Heat shock protein (Hsp) (60 and 90), corticosterone, and oxidative 
      stress biomarkers were determined in all the samples. Results Fetal and placental 
      weights in all genistein-exposed groups were significantly decreased. A 
      fluctuation in the level of the Hsp was recorded with a significant decrease 
      recorded in Hsp90 level in the placenta and amniotic fluid towards GD-20 along 
      with a concomitant increase in the corticosterone level in the amniotic fluid in 
      all genistein groups compared to control. Maternal serum at GD-18 and GD -20 
      recorded a significant increase in antioxidant level (SOD, GSH, CAT) in all 
      genistein-exposed groups. However, these antioxidants were significantly reduced 
      in the placenta and the amniotic fluid compared to control. Conclusions Genistein 
      enhances the placenta function in attenuating the risk of oxidative stress in the 
      amniotic fluid and deferentially suppressed inflammatory activities in the 
      placenta during early gestation and towards late gestation period.
FAU - Awobajo, Funmileyi O
AU  - Awobajo FO
AD  - Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, 
      University of Lagos, Room 006, Block F, Idi-araba, Surulere, Lagos, Nigeria, 
      Phone: +23408053416937.
FAU - Morakinyo, Ayodele O
AU  - Morakinyo AO
AD  - Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, 
      University of Lagos, Lagos, Nigeria.
FAU - Samuel, Titilola A
AU  - Samuel TA
AD  - Department of Biochemistry, Faculty of Basic Medical Sciences, College of 
      Medicine, University of Lagos, Lagos, Nigeria.
FAU - Oyelowo, Oluwakemi T
AU  - Oyelowo OT
AD  - Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, 
      University of Lagos, Lagos, Nigeria.
FAU - Ogunsola, Abimbola O
AU  - Ogunsola AO
AD  - Department of Physiology, Ben Carson School of Medicine, Babcock University, 
      Ilisan-Remo,Ogun State, Nigeria.
FAU - Onyekwele, Perpetual U
AU  - Onyekwele PU
AD  - Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, 
      University of Lagos, Lagos, Nigeria.
FAU - Okedina, Mosunmola E
AU  - Okedina ME
AD  - Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, 
      University of Lagos, Lagos, Nigeria.
FAU - Ogunbanwo, Oluwadamilola O
AU  - Ogunbanwo OO
AD  - Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, 
      University of Lagos, Lagos, Nigeria.
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Basic Clin Physiol Pharmacol
JT  - Journal of basic and clinical physiology and pharmacology
JID - 9101750
RN  - 0 (Inflammation Mediators)
RN  - 0 (Phytoestrogens)
RN  - DH2M523P0H (Genistein)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Amniotic Fluid/*drug effects/metabolism
MH  - Animals
MH  - Corticosterone/antagonists & inhibitors/blood
MH  - Female
MH  - Fetal Weight/drug effects/physiology
MH  - Genistein/*pharmacology
MH  - Inflammation/blood/prevention & control
MH  - Inflammation Mediators/*antagonists & inhibitors/metabolism
MH  - Maternal-Fetal Exchange/*drug effects/physiology
MH  - Organ Size/drug effects/physiology
MH  - Oxidative Stress/*drug effects/physiology
MH  - Phytoestrogens/pharmacology
MH  - Placenta/*drug effects/metabolism
MH  - Pregnancy
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - amniotic fluid
OT  - genistein
OT  - heat shock protein
OT  - inflammatory reaction
OT  - oxidative stress
OT  - placenta
EDAT- 2018/10/18 06:00
MHDA- 2019/06/22 06:00
CRDT- 2018/10/18 06:00
PHST- 2018/04/15 00:00 [received]
PHST- 2018/07/11 00:00 [accepted]
PHST- 2018/10/18 06:00 [pubmed]
PHST- 2019/06/22 06:00 [medline]
PHST- 2018/10/18 06:00 [entrez]
AID - /j/jbcpp.ahead-of-print/jbcpp-2018-0070/jbcpp-2018-0070.xml [pii]
AID - 10.1515/jbcpp-2018-0070 [doi]
PST - ppublish
SO  - J Basic Clin Physiol Pharmacol. 2018 Dec 19;30(1):37-45. doi: 
      10.1515/jbcpp-2018-0070.