PMID- 30333752
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 9
DP  - 2018
TI  - Trans-Chalcone Attenuates Pain and Inflammation in Experimental Acute Gout 
      Arthritis in Mice.
PG  - 1123
LID - 10.3389/fphar.2018.01123 [doi]
LID - 1123
AB  - Gouty arthritis is characterized by an intense inflammatory response to 
      monosodium urate crystals (MSU), which induces severe pain and reduction in the 
      life quality of patients. Trans-Chalcone (1,3-diphenyl-2-propen-1-one) is a 
      flavonoid precursor presenting biological activities such as anti-inflammatory 
      and antioxidant proprieties. Thus, the aim of this work was to evaluate the 
      protective effects of trans-Chalcone in experimental gout arthritis in mice. Mice 
      were treated with trans-Chalcone (3, 10, or 30 mg/kg, per oral) or vehicle (Tween 
      80 20% plus saline) 30 min before intra-articular injection of MSU (100 mug/knee 
      joint, intra-articular). We observed that trans-Chalcone inhibited MSU-induced 
      mechanical hyperalgesia, edema, and leukocyte recruitment (total leukocytes, 
      neutrophils, and mononuclear cells) in a dose-dependent manner. Trans-Chalcone 
      also decreased inflammatory cell recruitment as observed in Hematoxylin and Eosin 
      (HE) staining and the intensity of fluorescence of LysM-eGFP+ cells in the 
      confocal microscopy. Trans-Chalcone reduced MSU-induced oxidative stress as 
      observed by an increase in the antioxidant defense [Glutathione (GSH), Ferric 
      Reducing (FRAP), and 2,2'-Azinobis-3-ethylbenzothiazoline 6-sulfonic acid (ABTS 
      assays)] and reduction in reactive oxygen and nitrogen species production 
      [superoxide anion (NBT assay) and nitrite (NO assay)]. Furthermore, it reduced in 
      vivo MSU-induced interleukin-1beta (IL-1beta), Tumor necrosis factor-alpha (TNF-alpha), and 
      IL-6 production, and increased Transforming growth factor-beta (TGF-beta) production. 
      Importantly, trans-Chalcone reduced nuclear factor kappa-light-chain-enhancer of 
      activated B cells (NF-kappaB) activation and thereby the mRNA expression of the 
      inflammasome components Nlrp3 (cryopyrin), Asc (apoptosis-associated speck-like 
      protein containing a CARD), Pro-caspase-1 and Pro-IL-1beta. In vitro, trans-Chalcone 
      reduced the MSU-induced release of IL-1beta in lipopolysaccharide (LPS)-primed 
      macrophages. Therefore, the pharmacological effects of trans-Chalcone indicate 
      its therapeutic potential as an analgesic and anti-inflammatory flavonoid for the 
      treatment of gout.
FAU - Staurengo-Ferrari, Larissa
AU  - Staurengo-Ferrari L
AD  - Departamento de Ciencias Patologicas, Universidade Estadual de Londrina, 
      Londrina, Brazil.
FAU - Ruiz-Miyazawa, Kenji W
AU  - Ruiz-Miyazawa KW
AD  - Departamento de Ciencias Patologicas, Universidade Estadual de Londrina, 
      Londrina, Brazil.
FAU - Pinho-Ribeiro, Felipe A
AU  - Pinho-Ribeiro FA
AD  - Departamento de Ciencias Patologicas, Universidade Estadual de Londrina, 
      Londrina, Brazil.
FAU - Fattori, Victor
AU  - Fattori V
AD  - Departamento de Ciencias Patologicas, Universidade Estadual de Londrina, 
      Londrina, Brazil.
FAU - Zaninelli, Tiago H
AU  - Zaninelli TH
AD  - Departamento de Ciencias Patologicas, Universidade Estadual de Londrina, 
      Londrina, Brazil.
FAU - Badaro-Garcia, Stephanie
AU  - Badaro-Garcia S
AD  - Departamento de Ciencias Patologicas, Universidade Estadual de Londrina, 
      Londrina, Brazil.
FAU - Borghi, Sergio M
AU  - Borghi SM
AD  - Departamento de Ciencias Patologicas, Universidade Estadual de Londrina, 
      Londrina, Brazil.
FAU - Carvalho, Thacyana T
AU  - Carvalho TT
AD  - Departamento de Ciencias Patologicas, Universidade Estadual de Londrina, 
      Londrina, Brazil.
FAU - Alves-Filho, Jose C
AU  - Alves-Filho JC
AD  - Department of Pharmacology, Ribeirao Preto Medical School, University of Sao 
      Paulo, Ribeirao Preto, Brazil.
FAU - Cunha, Thiago M
AU  - Cunha TM
AD  - Department of Pharmacology, Ribeirao Preto Medical School, University of Sao 
      Paulo, Ribeirao Preto, Brazil.
FAU - Cunha, Fernando Q
AU  - Cunha FQ
AD  - Department of Pharmacology, Ribeirao Preto Medical School, University of Sao 
      Paulo, Ribeirao Preto, Brazil.
FAU - Casagrande, Rubia
AU  - Casagrande R
AD  - Departamento de Ciencias Farmaceuticas, Universidade Estadual de Londrina, 
      Londrina, Brazil.
FAU - Verri, Waldiceu A Jr
AU  - Verri WA Jr
AD  - Departamento de Ciencias Patologicas, Universidade Estadual de Londrina, 
      Londrina, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20181002
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC6176465
OTO - NOTNLM
OT  - flavonoids
OT  - gout flare
OT  - gouty arthritis
OT  - inflammation
OT  - joint pain
OT  - natural products
OT  - rheumatic disease
OT  - trans-Chalcone
EDAT- 2018/10/20 06:00
MHDA- 2018/10/20 06:01
PMCR- 2018/10/02
CRDT- 2018/10/19 06:00
PHST- 2018/06/16 00:00 [received]
PHST- 2018/09/13 00:00 [accepted]
PHST- 2018/10/19 06:00 [entrez]
PHST- 2018/10/20 06:00 [pubmed]
PHST- 2018/10/20 06:01 [medline]
PHST- 2018/10/02 00:00 [pmc-release]
AID - 10.3389/fphar.2018.01123 [doi]
PST - epublish
SO  - Front Pharmacol. 2018 Oct 2;9:1123. doi: 10.3389/fphar.2018.01123. eCollection 
      2018.