PMID- 3033490
OWN - NLM
STAT- MEDLINE
DCOM- 19870617
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 183
IP  - 3
DP  - 1987 May
TI  - Nitropyrenes are inducers of polyoma viral DNA synthesis.
PG  - 203-11
AB  - The biological activity of a series of nitropyrenes was assayed by measuring 
      their ability to induce the asynchronous replication of viral DNA in rat 
      fibroblasts transformed by a ts-a mutant of polyoma virus. Concentrations of 
      10-30 micrograms/ml of 1-nitropyrene (1-NP) induced viral replication, and this 
      effect was enhanced by addition of rat-liver S9 microsomal fraction (300 
      micrograms/ml) to the culture medium. The response was less than that obtained 
      with 0.1 micrograms/ml of the activated metabolite of benzo[a]pyrene (BP), BP 
      trans-7,8-dihydrodiol-9,10 epoxide (anti) (BPDE). A series of di-, tri-, and 
      tetra-nitropyrenes were also found to induce polyoma DNA replication, in the 
      absence of exogenous microsomal activation, displaying strongly positive effects 
      at 0.5-2.0 microgram/ml. Dose-response curves with 1,6-dinitropyrene (1,6-DNP) 
      from 0.01 to 0.5 microgram/ml indicated that this compound was approximately 
      equipotent with BPDE for induction of polyoma DNA synthesis. Studies of drug 
      metabolism, DNA binding and DNA adduct formation indicate that 1,6-DNP is 
      metabolized in this cell line, binds to DNA, and forms stable adducts. The level 
      of DNA modification seen with 1,6-DNP is higher than that observed under 
      comparable conditions with an equivalent dose of BPDE. These findings provide 
      additional evidence that the nitropyrene class of compounds can exert biological 
      effects in mammalian cells, and that the dinitropyrenes are more potent than 
      1-NP.
FAU - Lambert, M E
AU  - Lambert ME
FAU - Weinstein, I B
AU  - Weinstein IB
LA  - eng
GR  - CA0211/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA, Viral)
RN  - 0 (Pyrenes)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 55097-80-8 (7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide)
RN  - 66Q2ZUF83N (1,6-dinitropyrene)
SB  - IM
MH  - 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide/metabolism
MH  - Animals
MH  - Benzo(a)pyrene/metabolism/pharmacology
MH  - Biotransformation
MH  - Cell Line
MH  - *DNA Damage
MH  - DNA, Viral/*biosynthesis
MH  - Polyomavirus/drug effects/*genetics
MH  - Pyrenes/metabolism/*pharmacology
MH  - Rats
MH  - Structure-Activity Relationship
MH  - Virus Replication/drug effects
EDAT- 1987/05/01 00:00
MHDA- 1987/05/01 00:01
CRDT- 1987/05/01 00:00
PHST- 1987/05/01 00:00 [pubmed]
PHST- 1987/05/01 00:01 [medline]
PHST- 1987/05/01 00:00 [entrez]
AID - 0167-8817(87)90001-0 [pii]
AID - 10.1016/0167-8817(87)90001-0 [doi]
PST - ppublish
SO  - Mutat Res. 1987 May;183(3):203-11. doi: 10.1016/0167-8817(87)90001-0.