PMID- 30335529
OWN - NLM
STAT- MEDLINE
DCOM- 20190624
LR  - 20190624
IS  - 1532-4311 (Electronic)
IS  - 0882-0139 (Linking)
VI  - 48
IP  - 3
DP  - 2019 Apr
TI  - Comparative Analysis of Bone Marrow-derived Mast Cell Differentiation in C57BL/6 
      and BALB/c Mice.
PG  - 303-320
LID - 10.1080/08820139.2018.1523924 [doi]
AB  - Allergic diseases have increased in the last three decades. Mast cells play a 
      critical role in allergic diseases along with allergen-specific immunoglobulin E 
      (IgE). Following mast cell degranulation elicited by ligation of the IgE-FcepsilonRI 
      receptor complex with allergen, allergic reactions are followed by various 
      symptoms such as vascular hyperpermeability, mucous secretion, itching, sneezing, 
      wheezing, rashes, fever, and anaphylactic shock. Susceptibility or inclination to 
      allergy varies depending on individual genetic traits and living environment, and 
      it has long been believed that such an inclination is determined by an 
      immunologic balance of T helper cell types. Mouse strains also have different 
      susceptibilities to allergy. Similar to T helper cells and macrophages, it is not 
      known whether mast cells can also be divided into two different types between 
      mouse strains. In this study, we prepared bone marrow-derived mast cells from 
      BALB/c and C57BL/6 mice and examined their cellular properties. Cellular response 
      to IL-3 and the process of mast cell differentiation from bone marrow cells were 
      different on the basis of cell surface marker molecules. BALB/c-derived cells 
      more efficiently exhibited degranulation than did C57BL/6-derived cells following 
      both calcium ionophore and receptor crosslinking. These functional differences 
      persisted even after a longer cell culture for 8 weeks, suggesting a difference 
      in cell-autonomous characteristics. These results support the concept that mast 
      cells also have different cell types dependent on their genetic background. 
      Abbreviations: Ab: antibody; BMMC: bone marrow-derived mast cell; DNP: 
      dinitrophenyl; FACS: fluorescence-activated cell sorter; FCS: fetal calf serum; 
      FITC: fluorescein isothiocyanate; FSC: forward scatter; HRP: horseradish 
      peroxidase; HSA: human serum albumin; Ig: immunoglobulin; IL: interleukin; MIP-2: 
      macrophage inflammatory protein-2; MCP: mast cell protease; PE: phycoerythrin; 
      PerCP: Peridinin chlorophyll protein complex; SNP: single nucleotide 
      polymorphisms; SSC: side scatter; Th: T helper; TNF-alpha: tumor necrosis factor 
      alpha.
FAU - Nagashima, Miki
AU  - Nagashima M
AD  - a Host Defense for Animals , Nippon Veterinary and Life Science University , 
      Musashino-shi, Tokyo , Japan.
FAU - Koyanagi, Madoka
AU  - Koyanagi M
AD  - a Host Defense for Animals , Nippon Veterinary and Life Science University , 
      Musashino-shi, Tokyo , Japan.
FAU - Arimura, Yutaka
AU  - Arimura Y
AD  - a Host Defense for Animals , Nippon Veterinary and Life Science University , 
      Musashino-shi, Tokyo , Japan.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20181018
PL  - England
TA  - Immunol Invest
JT  - Immunological investigations
JID - 8504629
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*physiology
MH  - Cell Degranulation/immunology
MH  - Cell Differentiation/*immunology
MH  - Cell Line
MH  - Disease Models, Animal
MH  - Humans
MH  - Hypersensitivity/immunology
MH  - Mast Cells/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C/*immunology
MH  - Mice, Inbred C57BL/*immunology
MH  - Primary Cell Culture
OTO - NOTNLM
OT  - Allergy
OT  - degranulation
OT  - interleukin-3
OT  - mast cell
EDAT- 2018/10/20 06:00
MHDA- 2019/06/25 06:00
CRDT- 2018/10/19 06:00
PHST- 2018/10/20 06:00 [pubmed]
PHST- 2019/06/25 06:00 [medline]
PHST- 2018/10/19 06:00 [entrez]
AID - 10.1080/08820139.2018.1523924 [doi]
PST - ppublish
SO  - Immunol Invest. 2019 Apr;48(3):303-320. doi: 10.1080/08820139.2018.1523924. Epub 
      2018 Oct 18.