PMID- 30335544
OWN - NLM
STAT- MEDLINE
DCOM- 20181219
LR  - 20181219
IS  - 1369-1635 (Electronic)
IS  - 0953-7104 (Linking)
VI  - 29
IP  - 8
DP  - 2018 Dec
TI  - Sushi Domain Containing 2 (SUSD2) inhibits platelet activation and binding to 
      high-grade serous ovarian carcinoma cells.
PG  - 834-837
LID - 10.1080/09537104.2018.1530345 [doi]
AB  - Platelets play a central role in primary hemostasis affecting tumor survival and 
      metastases. Tumors induce platelets to aggregate and bind to the cancer cells, 
      resulting in protection from immune surveillance and often leading to 
      thrombocytosis. In ovarian cancer (OvCa), one-third of patients present with 
      thrombocytosis, a diagnosis that correlates with shorter survival. SUSD2 (SUShi 
      Domain containing 2), a type I transmembrane protein, shown to inhibit metastatic 
      processes in high-grade serous ovarian carcinoma (HGSOC), is expressed on 
      endothelial cells and thus may influence platelet reactivity. As such, we 
      hypothesized that SUSD2 levels in ovarian cancer-derived cell lines influence 
      platelet activation. We incubated OvCa non-targeting (NT) and SUSD2 knockdown 
      (KD) cell lines with labeled platelets and quantified platelet binding, as well 
      as GPIIb/IIIa integrin activation. The role of GPIIb/IIIa in tumor cell/platelet 
      interaction was also examined by measuring cell-cell adhesion in the presence of 
      eptifibatide. We found that platelets exposed to OvCa cells with low SUSD2 
      expression display increased tumor cell-platelet binding along with an increase 
      in GPIIb/IIIa receptor activation. As such, platelet activation and binding to 
      HGSOC cells was inversely correlated with the presence of SUSD2. This represents 
      one of the first tumor proteins known to provide differential platelet 
      interaction based on protein status.
FAU - Lager, Tyson W
AU  - Lager TW
AD  - a Department of Biology , Augustana University , Sioux Falls , SD , USA.
FAU - Roetman, Jessica J
AU  - Roetman JJ
AD  - a Department of Biology , Augustana University , Sioux Falls , SD , USA.
FAU - Kunkel, Jacob
AU  - Kunkel J
AD  - a Department of Biology , Augustana University , Sioux Falls , SD , USA.
FAU - Thacker, Megan
AU  - Thacker M
AD  - a Department of Biology , Augustana University , Sioux Falls , SD , USA.
FAU - Sheets, Jordan N
AU  - Sheets JN
AD  - b Cancer Biology Research Center, Sanford Research , Sanford School of Medicine 
      of the University of South Dakota , Sioux Falls , SD , USA.
FAU - Egland, Kristi A
AU  - Egland KA
AD  - b Cancer Biology Research Center, Sanford Research , Sanford School of Medicine 
      of the University of South Dakota , Sioux Falls , SD , USA.
FAU - Miles, Cecelia M
AU  - Miles CM
AD  - a Department of Biology , Augustana University , Sioux Falls , SD , USA.
FAU - Larson, Mark K
AU  - Larson MK
AD  - a Department of Biology , Augustana University , Sioux Falls , SD , USA.
FAU - Gubbels, Jennifer A A
AU  - Gubbels JAA
AUID- ORCID: 0000-0001-9878-0065
AD  - a Department of Biology , Augustana University , Sioux Falls , SD , USA.
LA  - eng
PT  - Journal Article
DEP - 20181018
PL  - England
TA  - Platelets
JT  - Platelets
JID - 9208117
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (SUSD2 protein, human)
SB  - IM
MH  - Blood Platelets/*metabolism/pathology
MH  - Cell Adhesion
MH  - Cell Line, Tumor
MH  - Coculture Techniques
MH  - Female
MH  - Humans
MH  - Membrane Glycoproteins/*metabolism
MH  - Neoplasm Proteins/*metabolism
MH  - Ovarian Neoplasms/*metabolism/pathology
MH  - *Platelet Activation
OTO - NOTNLM
OT  - GPIIb/IIIa
OT  - SUSD2
OT  - ovarian cancer
EDAT- 2018/10/20 06:00
MHDA- 2018/12/20 06:00
CRDT- 2018/10/19 06:00
PHST- 2018/10/20 06:00 [pubmed]
PHST- 2018/12/20 06:00 [medline]
PHST- 2018/10/19 06:00 [entrez]
AID - 10.1080/09537104.2018.1530345 [doi]
PST - ppublish
SO  - Platelets. 2018 Dec;29(8):834-837. doi: 10.1080/09537104.2018.1530345. Epub 2018 
      Oct 18.