PMID- 30336190
OWN - NLM
STAT- MEDLINE
DCOM- 20190307
LR  - 20190307
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 393
DP  - 2018 Nov 21
TI  - Expression of Pituitary Adenylate Cyclase-activating Peptide, Calcitonin 
      Gene-related Peptide and Headache Targets in the Trigeminal Ganglia of Rats and 
      Humans.
PG  - 319-332
LID - S0306-4522(18)30665-1 [pii]
LID - 10.1016/j.neuroscience.2018.10.004 [doi]
AB  - Neurotransmitter and headache target localization in the trigeminal ganglia (TG) 
      might increase the understanding of sites of action, and mechanisms related to 
      headache therapy. The overall aim of the study was to investigate the presence of 
      migraine targets in the TG with particular emphasis on pituitary adenylate 
      cyclase-activating peptide (PACAP) and calcitonin gene-related peptide (CGRP), 
      known to be involved in cranial pain processing, and selected headache targets. 
      Rat- and human TG were processed for immunohistochemistry. PACAP-38, CGRP and the 
      headache targets were expressed in rat and human TG. PACAP receptors were 
      confined to neurons and satellite glial cells (SGCs), however with variability 
      between the receptor subtypes PACAP type I receptor (PAC(1)) and vasoactive 
      intestinal peptide/PACAP receptors 1/2 (VPAC(1/2)). 5-Hydroxytryptamine (5-HT) 
      receptors were expressed in neuronal somas in rat and human TG (human TG 
      frequency: 5-HT(1D) > 5-HT(1B/1F)). Synaptosomal-associated protein 25 kDa 
      (SNAP25) was primarily expressed in SGCs in humans, and neurons in rats, while 
      synaptic vesicle glycoprotein 2A (SV2-A) was confined to SGCs and some neurons in 
      rats and humans. Occasionally, PACAP-38-positive cells also expressed VPAC(1), 
      SNAP25 and SV2-A. VPAC(1) was generally detected in SGCs enveloping 
      PACAP-38-positive and -negative neuronal somas. Our study revealed potential 
      sites of actions for anti-headache drugs such as PACAP receptor antagonists, 
      Lasmiditan (5-HT(1F) agonist) and Botox (blocks exocytosis through SV2-A/SNAP25) 
      in rat and human TG and considerable overlap between species in expression to 
      specific cell types, except for VPAC(1) and SNAP25.
CI  - Copyright (c) 2018 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Frederiksen, Simona Denise
AU  - Frederiksen SD
AD  - Department of Clinical Experimental Research, Glostrup Research Institute, 
      Rigshospitalet Glostrup, 2600 Glostrup, Denmark; Division of Experimental 
      Vascular Research, Department of Clinical Sciences, Lund University, 221 84 Lund, 
      Sweden. Electronic address: simona.frederiksen@hotmail.com.
FAU - Warfvinge, Karin
AU  - Warfvinge K
AD  - Department of Clinical Experimental Research, Glostrup Research Institute, 
      Rigshospitalet Glostrup, 2600 Glostrup, Denmark; Division of Experimental 
      Vascular Research, Department of Clinical Sciences, Lund University, 221 84 Lund, 
      Sweden. Electronic address: karin.warfvinge@med.lu.se.
FAU - Ohlsson, Lena
AU  - Ohlsson L
AD  - Division of Experimental Vascular Research, Department of Clinical Sciences, Lund 
      University, 221 84 Lund, Sweden. Electronic address: lena.ohlsson@med.lu.se.
FAU - Edvinsson, Lars
AU  - Edvinsson L
AD  - Department of Clinical Experimental Research, Glostrup Research Institute, 
      Rigshospitalet Glostrup, 2600 Glostrup, Denmark; Division of Experimental 
      Vascular Research, Department of Clinical Sciences, Lund University, 221 84 Lund, 
      Sweden. Electronic address: lars.edvinsson@med.lu.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181015
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Pituitary Adenylate Cyclase-Activating Polypeptide)
RN  - 0 (Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - 9007-12-9 (Calcitonin)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Adenylyl Cyclases/metabolism
MH  - Animals
MH  - Calcitonin/genetics
MH  - Calcitonin Gene-Related Peptide/*metabolism
MH  - Headache/*metabolism
MH  - Humans
MH  - Male
MH  - Migraine Disorders/metabolism
MH  - Neurons/metabolism
MH  - Pituitary Adenylate Cyclase-Activating Polypeptide/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide/metabolism
MH  - Trigeminal Ganglion/*metabolism
MH  - Vasoactive Intestinal Peptide/metabolism
OTO - NOTNLM
OT  - Botox
OT  - CGRP
OT  - PACAP
OT  - PACAP receptors
OT  - sensory nervous system
OT  - serotonin receptors
EDAT- 2018/10/20 06:00
MHDA- 2019/03/08 06:00
CRDT- 2018/10/19 06:00
PHST- 2018/08/21 00:00 [received]
PHST- 2018/09/28 00:00 [revised]
PHST- 2018/10/02 00:00 [accepted]
PHST- 2018/10/20 06:00 [pubmed]
PHST- 2019/03/08 06:00 [medline]
PHST- 2018/10/19 06:00 [entrez]
AID - S0306-4522(18)30665-1 [pii]
AID - 10.1016/j.neuroscience.2018.10.004 [doi]
PST - ppublish
SO  - Neuroscience. 2018 Nov 21;393:319-332. doi: 10.1016/j.neuroscience.2018.10.004. 
      Epub 2018 Oct 15.