PMID- 30338812
OWN - NLM
STAT- MEDLINE
DCOM- 20191212
LR  - 20191217
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 22
IP  - 19
DP  - 2018 Oct
TI  - Transplantation of Human umbilical cord mesenchymal stem cells promotes 
      functional recovery after spinal cord injury by blocking the expression of IL-7.
PG  - 6436-6447
LID - 16056 [pii]
LID - 10.26355/eurrev_201810_16056 [doi]
AB  - OBJECTIVE: This research aimed to investigate the therapeutic effects of 
      transplanted human umbilical cord mesenchymal stem cells (hUCMSCs) on spinal cord 
      injury in mice and to explore its molecular mechanism. MATERIALS AND METHODS: 
      Spinal cord injury model in C57BL/6J mice was established. On the 10th day of 
      SCI, hUCMSCs were injected into the center of spinal cord injury area (hUCMSC), 
      and control groups (Control) were injected with an equal amount of medium. 
      Western blotting, Real Time-PCR, immunohistochemistry, and flow cytometry, were 
      used to analyze the content of IL-7, inflammatory cytokines, and macrophages 
      after spinal cord injury in different groups. Open field and Rota-Rod tests were 
      used to determine the effect of hUCMSC transplantation on motor function recovery 
      in SCI mice. RESULTS: Compared with the control mice, hUCMSC transplantation 
      therapy significantly improved the motor function, myelin, and nerve cell 
      survival in spinal cord injury site in SCI mice. It also reduced the expression 
      of IL-7, IFN-gamma, and TNF-alpha in injured sites but increased IL-4 and IL-13 
      expression and promoted the activation of M2 macrophages at the site of injury. 
      CONCLUSIONS: Transplantation of hUCMSCs in SCI mice can promote the polarization 
      of M2 macrophages by reducing the expression of IL-7 in the injured site, thereby 
      weakening the inflammatory response at the injured site, promoting the repair of 
      the injured site and improving the motor function.
FAU - Bao, C-S
AU  - Bao CS
AD  - Department of Neurosurgery, Southwest Medical University Affiliated Hospital, 
      Neurosurgical Clinical Research Center of Sichuan Province, Luzhou, Sichuan 
      Province, China. chenligangclg169@163.com.
FAU - Li, X-L
AU  - Li XL
FAU - Liu, L
AU  - Liu L
FAU - Wang, B
AU  - Wang B
FAU - Yang, F-B
AU  - Yang FB
FAU - Chen, L-G
AU  - Chen LG
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Interleukin-7)
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - Cells, Cultured
MH  - *Cord Blood Stem Cell Transplantation
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Female
MH  - Humans
MH  - Interleukin-7/genetics/*metabolism
MH  - Macrophage Activation
MH  - Macrophages/*metabolism
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Motor Activity
MH  - Phenotype
MH  - Recovery of Function
MH  - Spinal Cord/metabolism/physiopathology/*surgery
MH  - Spinal Cord Injuries/genetics/metabolism/physiopathology/*surgery
EDAT- 2018/10/20 06:00
MHDA- 2019/12/18 06:00
CRDT- 2018/10/20 06:00
PHST- 2018/10/20 06:00 [entrez]
PHST- 2018/10/20 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
AID - 16056 [pii]
AID - 10.26355/eurrev_201810_16056 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2018 Oct;22(19):6436-6447. doi: 
      10.26355/eurrev_201810_16056.