PMID- 30339045
OWN - NLM
STAT- MEDLINE
DCOM- 20200501
LR  - 20200501
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 44
IP  - 3
DP  - 2019 Mar
TI  - Evaluation of the Suitability of Biocompatible Carriers as Artificial Transplants 
      Using Cultured Porcine Corneal Endothelial Cells.
PG  - 243-249
LID - 10.1080/02713683.2018.1536215 [doi]
AB  - Purpose/Aim: Evaluating the suitability of bioengineered collagen sheets and 
      human anterior lens capsules (HALCs) as carriers for cultivated porcine corneal 
      endothelial cells (pCECs) and in vitro assessment of the cell-carrier sheets as 
      tissue-engineered grafts for Descemet membrane endothelial keratoplasty (DMEK). 
      MATERIALS AND METHODS: pCECs were isolated, cultured up to P2 and seeded onto 
      LinkCell bioengineered matrices of 20 microm (LK20) or 100 microm (LK100) thickness, and 
      on HALC. During expansion, pCEC viability and morphology were assessed by light 
      microscopy. ZO-1 and Na(+)/K(+)-ATPase expression was investigated by 
      immunohistochemistry. Biomechanical properties of pCEC-carrier constructs were 
      evaluated by simulating DMEK surgery in vitro using an artificial anterior 
      chamber (AC) and a human donor cornea without Descemet membrane (DM). RESULTS: 
      During in vitro expansion, cultured pCECs retained their proliferative capacity, 
      as shown by the positive staining for proliferative marker Ki67, and a high cell 
      viability rate (96 +/- 5%). pCECs seeded on all carriers formed a monolayer of 
      hexagonal, tightly packed cells that expressed ZO-1 and Na(+)/K(+)-ATPase. During 
      in vitro surgery, pCEC-LK20 and pCEC-LK100 constructs were handled like Descemet 
      stripping endothelial keratoplasty (DSEK) grafts, i.e. folded like a "taco" for 
      insertion because of challenges related to rolling and sticking of the grafts in 
      the injector. pCEC-HALC constructs behaved similar to the DMEK reference model 
      during implantation and unfolding in the artificial AC, showing good adhesion to 
      the bare stroma. CONCLUSIONS: In vitro DMEK surgery showed HALC as the most 
      suitable carrier for cultivated pCECs with good intraoperative graft handling. 
      LK20 carrier showed good biocompatibility, but required a DSEK-adapted surgical 
      protocol. Both carriers might be notional candidates for potential future 
      clinical applications.
FAU - Spinozzi, Daniele
AU  - Spinozzi D
AD  - a Netherlands Institute for Innovative Ocular Surgery , Rotterdam , The 
      Netherlands.
FAU - Miron, Alina
AU  - Miron A
AD  - a Netherlands Institute for Innovative Ocular Surgery , Rotterdam , The 
      Netherlands.
FAU - Bruinsma, Marieke
AU  - Bruinsma M
AD  - a Netherlands Institute for Innovative Ocular Surgery , Rotterdam , The 
      Netherlands.
FAU - Dapena, Isabel
AU  - Dapena I
AD  - a Netherlands Institute for Innovative Ocular Surgery , Rotterdam , The 
      Netherlands.
AD  - b Melles Cornea Clinic Rotterdam , Rotterdam , The Netherlands.
FAU - Lavy, Itay
AU  - Lavy I
AD  - a Netherlands Institute for Innovative Ocular Surgery , Rotterdam , The 
      Netherlands.
AD  - b Melles Cornea Clinic Rotterdam , Rotterdam , The Netherlands.
FAU - Binder, Perry S
AU  - Binder PS
AD  - c Gavin Herbert Eye Institute , University of California , Irvine , California , 
      USA.
FAU - Rafat, Mehrdad
AU  - Rafat M
AD  - d LinkoCare Life Science AB , Linkoping , Sweden.
AD  - e Department of Biomedical Engineering , Linkoping University , Linkoping , 
      Sweden.
FAU - Oellerich, Silke
AU  - Oellerich S
AD  - a Netherlands Institute for Innovative Ocular Surgery , Rotterdam , The 
      Netherlands.
FAU - Melles, Gerrit R J
AU  - Melles GRJ
AD  - a Netherlands Institute for Innovative Ocular Surgery , Rotterdam , The 
      Netherlands.
AD  - b Melles Cornea Clinic Rotterdam , Rotterdam , The Netherlands.
AD  - f Amnitrans EyeBank Rotterdam , Rotterdam , The Netherlands.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181207
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Biocompatible Materials)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 9007-34-5 (Collagen)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Animals
MH  - *Biocompatible Materials
MH  - Cell Proliferation/physiology
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - *Collagen
MH  - Descemet Stripping Endothelial Keratoplasty
MH  - Endothelium, Corneal/*cytology/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Ki-67 Antigen/metabolism
MH  - *Lens Capsule, Crystalline
MH  - Sodium-Potassium-Exchanging ATPase/metabolism
MH  - Swine
MH  - Tissue Engineering
MH  - *Tissue Scaffolds
MH  - Zonula Occludens-1 Protein/metabolism
OTO - NOTNLM
OT  - Porcine endothelial cells
OT  - cell carriers
OT  - cell culture
OT  - donor material
OT  - endothelial cell transplantation
EDAT- 2018/10/20 06:00
MHDA- 2020/05/02 06:00
CRDT- 2018/10/20 06:00
PHST- 2018/10/20 06:00 [pubmed]
PHST- 2020/05/02 06:00 [medline]
PHST- 2018/10/20 06:00 [entrez]
AID - 10.1080/02713683.2018.1536215 [doi]
PST - ppublish
SO  - Curr Eye Res. 2019 Mar;44(3):243-249. doi: 10.1080/02713683.2018.1536215. Epub 
      2018 Dec 7.