PMID- 30342306
OWN - NLM
STAT- MEDLINE
DCOM- 20190420
LR  - 20240229
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 97
DP  - 2019 Jan
TI  - Topical application of phenytoin or nifedipine-loaded PLGA microspheres promotes 
      periodontal regeneration in vivo.
PG  - 42-51
LID - S0003-9969(18)30216-4 [pii]
LID - 10.1016/j.archoralbio.2018.10.002 [doi]
AB  - OBJECTIVES: Gingival recession and alveolar bone loss are common manifestations 
      of periodontitis. Periodontal regeneration is the ideal strategy for 
      rehabilitating periodontal tissue defects and preventing tooth loss. The present 
      study examined whether localized, topical application of gingival 
      overgrowth-inducing drugs, phenytoin, nifedipine or cyclosporine, induces 
      periodontal regeneration. METHODS: Polylactic-co-glycolic acid (PLGA) was used as 
      the carrier for preparation of phenytoin, nifedipine or cyclosporine-loaded PLGA 
      microspheres, using an oil-in-water emulsification technique. The drug-loaded 
      microspheres were delivered to periodontal defects created on alveolar ridges 
      mesial to the first maxillary molars of Sprague-Dawley rats. After eight weeks, 
      the operation area in each rat, including the maxillary molars and periodontal 
      tissues, was harvested and evaluated by micro-computed tomography, histochemical 
      and immunohistochemical analyses. RESULTS: Physical parameters representative of 
      periodontal regeneration, including the length of new alveolar bone (p < 0.01) 
      and the area of new alveolar bone (p < 0.01) were significantly improved in the 
      phenytoin group. Compared to other groups, the phenytoin group demonstrated 
      increased expression of COL-1, VEGF-A, osteoblast and osteoclast markers (BMP-2, 
      TGF-beta1, OCN and TRAP staining), as well as decreased expression of MMP-8. 
      CONCLUSIONS: Results of the present study provided new evidence that localized, 
      controlled release of phenytoin confers therapeutic benefits toward gingival 
      recession and alveolar bone loss. Phenytoin appears to be a promising drug that 
      promotes periodontal regeneration.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Zhao, Xiao-Heng
AU  - Zhao XH
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & 
      Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM), School and 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, PR China.
FAU - Tay, Franklin R
AU  - Tay FR
AD  - College of Graduate Studies, Augusta University, Augusta, GA, USA.
FAU - Fang, Yan-Jun
AU  - Fang YJ
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & 
      Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM), School and 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, PR China.
FAU - Meng, Liu-Yan
AU  - Meng LY
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & 
      Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM), School and 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, PR China. Electronic 
      address: mengliuyan@whu.edu.cn.
FAU - Bian, Zhuan
AU  - Bian Z
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & 
      Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM), School and 
      Hospital of Stomatology, Wuhan University, Wuhan, Hubei, PR China. Electronic 
      address: bianzhuan@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20181006
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Biomarkers)
RN  - 83HN0GTJ6D (Cyclosporine)
RN  - 0 (Drug Carriers)
RN  - I9ZF7L6G2L (Nifedipine)
RN  - 6158TKW0C5 (Phenytoin)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 0 (Polyesters)
SB  - IM
MH  - Animals
MH  - Male
MH  - Rats
MH  - Administration, Topical
MH  - *Alveolar Bone Loss/drug therapy
MH  - Biomarkers/analysis
MH  - Cyclosporine/administration & dosage
MH  - *Drug Carriers/administration & dosage
MH  - *Gingival Recession/drug therapy
MH  - Immunohistochemistry
MH  - *Microspheres
MH  - *Nifedipine/administration & dosage
MH  - *Phenytoin/administration & dosage
MH  - *Polyesters/administration & dosage
MH  - Random Allocation
MH  - Rats, Sprague-Dawley
MH  - Single-Blind Method
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - Alveolar bone loss
OT  - Anti-epileptics
OT  - Bone remodeling
OT  - Drug delivery
OT  - Gingival overgrowth
EDAT- 2018/10/21 06:00
MHDA- 2019/04/20 06:00
CRDT- 2018/10/21 06:00
PHST- 2018/06/04 00:00 [received]
PHST- 2018/09/13 00:00 [revised]
PHST- 2018/10/02 00:00 [accepted]
PHST- 2018/10/21 06:00 [pubmed]
PHST- 2019/04/20 06:00 [medline]
PHST- 2018/10/21 06:00 [entrez]
AID - S0003-9969(18)30216-4 [pii]
AID - 10.1016/j.archoralbio.2018.10.002 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2019 Jan;97:42-51. doi: 10.1016/j.archoralbio.2018.10.002. Epub 
      2018 Oct 6.