PMID- 30342358
OWN - NLM
STAT- MEDLINE
DCOM- 20190102
LR  - 20190102
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 167
DP  - 2019 Jan 15
TI  - Development of three-dimensional (3D) spheroid cultures of the continuous rainbow 
      trout liver cell line RTL-W1.
PG  - 250-258
LID - S0147-6513(18)31008-X [pii]
LID - 10.1016/j.ecoenv.2018.10.009 [doi]
AB  - In vitro experimental systems based on continuous piscine cell lines can be used 
      as an alternative to animal tests for obtaining qualitative and quantitative 
      information on the possible fate and effect of chemicals in fish. However, their 
      capability to reproduce complex metabolic processes and toxic responses as they 
      occur in vivo is limited due to the lack of organ-specific tissue architecture 
      and functions. Here we introduce a three-dimensional (3D) in vitro experimental 
      system based on spheroidal aggregate cultures (spheroids) of the continuous 
      rainbow trout liver cell line RTL-W1 and provide a first description of their 
      structural and functional properties including growth, viability/longevity, 
      metabolic activity, ultrastructure and cytochrome P450 1A (CYP1A) expression 
      determined by bright-field, multi-photon fluorescence and transmission electron 
      microscopy as well as RT-qPCR analysis. Our results show that RTL-W1 cells in 3D 
      spheroids (o ~ 150 microm) (including those in the interior) were viable, 
      metabolically active and had higher basal and beta-naphthoflavone-induced CYP1A 
      expression levels than conventional 2D cell cultures. Furthermore, they displayed 
      ultrastructural characteristics similar to differentiated hepatocytes. The 
      available evidence suggests that 3D RTL-W1 spheroids may have enhanced 
      hepatotypic functions and be a superior in vitro model to assess hepatic 
      biotransformation, bioaccumulation and chronic toxicity compared to conventional 
      cell monolayer cultures.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Lammel, Tobias
AU  - Lammel T
AD  - Department of Biological and Environmental Sciences, University of Gothenburg, 
      Medicinaregatan 18 A, Box 463, 413 90 Goteborg, Sweden. Electronic address: 
      tobias.lammel@gu.se.
FAU - Tsoukatou, Georgia
AU  - Tsoukatou G
AD  - Department of Biological and Environmental Sciences, University of Gothenburg, 
      Medicinaregatan 18 A, Box 463, 413 90 Goteborg, Sweden.
FAU - Jellinek, Johanna
AU  - Jellinek J
AD  - Department of Biological and Environmental Sciences, University of Gothenburg, 
      Medicinaregatan 18 A, Box 463, 413 90 Goteborg, Sweden.
FAU - Sturve, Joachim
AU  - Sturve J
AD  - Department of Biological and Environmental Sciences, University of Gothenburg, 
      Medicinaregatan 18 A, Box 463, 413 90 Goteborg, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20181017
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (RNA, Messenger)
RN  - 6051-87-2 (beta-Naphthoflavone)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/*methods
MH  - Cell Survival
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - *Hepatocytes/metabolism/ultrastructure
MH  - Liver/*cytology/drug effects
MH  - Oncorhynchus mykiss/*physiology
MH  - RNA, Messenger/metabolism
MH  - *Spheroids, Cellular/metabolism/ultrastructure
MH  - beta-Naphthoflavone/metabolism
OTO - NOTNLM
OT  - Aquatic toxicity
OT  - Bioaccumulation
OT  - Cytochrome P450 1A
OT  - Fish cell line
OT  - Hepatocyte
OT  - In vitro model
EDAT- 2018/10/21 06:00
MHDA- 2019/01/03 06:00
CRDT- 2018/10/21 06:00
PHST- 2018/06/12 00:00 [received]
PHST- 2018/09/29 00:00 [revised]
PHST- 2018/10/02 00:00 [accepted]
PHST- 2018/10/21 06:00 [pubmed]
PHST- 2019/01/03 06:00 [medline]
PHST- 2018/10/21 06:00 [entrez]
AID - S0147-6513(18)31008-X [pii]
AID - 10.1016/j.ecoenv.2018.10.009 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2019 Jan 15;167:250-258. doi: 
      10.1016/j.ecoenv.2018.10.009. Epub 2018 Oct 17.