PMID- 30343645 OWN - NLM STAT- MEDLINE DCOM- 20190124 LR - 20200225 IS - 1943-4936 (Electronic) IS - 1040-6387 (Print) IS - 1040-6387 (Linking) VI - 30 IP - 6 DP - 2018 Nov TI - Evaluation of an in-clinic dry chemistry analyzer for canine, equine, and feline plasma samples. PG - 902-910 LID - 10.1177/1040638718809407 [doi] AB - Method validation studies characterize the performance of new laboratory methods relative to established methods using quality guidelines in order to define the new method's performance characteristics and to identify differences that could influence data interpretation. We investigated the performance of an in-clinic dry chemistry analyzer (Catalyst One, IDEXX) for measuring 19 routine plasma biochemistry analytes in dogs, cats, and horses. We analyzed 2 levels of quality control material (QCM) in duplicate twice daily for 5 d to determine the coefficient of variation (CV), percent bias, observed total error (TE(obs)), and sigma metric (sigma) for each analyte at each level of QCM. We analyzed 82 canine, equine, and feline plasma samples with the in-clinic dry chemistry analyzer and a reference wet chemistry analyzer, and results were compared using correlation coefficients, Deming regression, and Bland-Altman analyses. CVs were <5% for 16 analytes and ⩾5% for 3 analytes. TE(obs) was less than allowable total error (TE(a)) for 9 analytes, and exceeded TE(a) for 10 analytes. Sigma metrics were >4 at both levels of QCM for 5 analytes, and at one level of QCM for 5 analytes; sigma metrics were <3 or could not be calculated at the remaining analyte concentrations. All analytes, except glucose, showed various magnitudes of bias compared to the wet chemistry analyzer. Based on these results, we recommend statistical (5 analytes) and non-statistical (14 analytes) QC measures and analyzer-specific reference intervals. FAU - Boes, Katie M AU - Boes KM AD - Department of Biomedical Sciences and Pathobiology (Boes), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Veterinary Teaching Hospital (Sink), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Department of Population Health Sciences (Werre), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA (Camus). FAU - Sink, Carolyn A AU - Sink CA AD - Department of Biomedical Sciences and Pathobiology (Boes), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Veterinary Teaching Hospital (Sink), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Department of Population Health Sciences (Werre), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA (Camus). FAU - Camus, Melinda S AU - Camus MS AD - Department of Biomedical Sciences and Pathobiology (Boes), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Veterinary Teaching Hospital (Sink), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Department of Population Health Sciences (Werre), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA (Camus). FAU - Werre, Stephen R AU - Werre SR AD - Department of Biomedical Sciences and Pathobiology (Boes), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Veterinary Teaching Hospital (Sink), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Department of Population Health Sciences (Werre), Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA. AD - Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA (Camus). LA - eng PT - Evaluation Study PT - Journal Article DEP - 20181020 PL - United States TA - J Vet Diagn Invest JT - Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc JID - 9011490 SB - IM MH - Animals MH - Blood Chemical Analysis/*veterinary MH - Cats/blood MH - Dogs/blood MH - Horses/blood MH - *Plasma MH - Reference Values MH - Reproducibility of Results PMC - PMC6505834 OTO - NOTNLM OT - Catalyst One OT - clinical chemistry tests OT - point-of-care systems OT - quality control COIS- Declaration of conflicting interests: KM Boes and C Sink declare that the in-clinic dry chemistry analyzer was provided to the Virginia-Maryland College of Veterinary Medicine by the manufacturer at a discounted rate, and that some test cartridges were also gifted by the manufacturer. M Camus and S Werre declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2018/10/23 06:00 MHDA- 2019/01/25 06:00 PMCR- 2019/10/20 CRDT- 2018/10/23 06:00 PHST- 2018/10/23 06:00 [pubmed] PHST- 2019/01/25 06:00 [medline] PHST- 2018/10/23 06:00 [entrez] PHST- 2019/10/20 00:00 [pmc-release] AID - 10.1177_1040638718809407 [pii] AID - 10.1177/1040638718809407 [doi] PST - ppublish SO - J Vet Diagn Invest. 2018 Nov;30(6):902-910. doi: 10.1177/1040638718809407. Epub 2018 Oct 20.