PMID- 30344286
OWN - NLM
STAT- MEDLINE
DCOM- 20191004
LR  - 20230928
IS  - 1648-9144 (Electronic)
IS  - 1010-660X (Print)
IS  - 1010-660X (Linking)
VI  - 54
IP  - 4
DP  - 2018 Jul 31
TI  - Evaluation of the Sleep-Prolonging Effect of Lagenaria vulgaris and Cucurbita 
      pepo Extracts on Pentobarbital-Induced Sleep and Possible Mechanisms of Action.
LID - 10.3390/medicina54040055 [doi]
LID - 55
AB  - Introduction: Sleeplessness is the most common sleep disorder. In this study, the 
      hypnotic effect of macerated (HAME) and soxhlet (HASE) extract of Lagenaria 
      vulgaris (fruit and seed) and Cucurbita pepo (fruit) were studied in mice. 
      Methods: Extracts and fractions were administered intra-peritoneally (i.p.) in 
      mice 30 min before the sodium pentobarbital (30 mg/kg, i.p.). Moreover, the 
      influence of flumazenil or naloxone on the hypnotic effects of the extract and 
      its toxic effects were evaluated. Results: The HAME and HASE of C. pepo prolonged 
      the pentobarbital-induced sleep duration at dose of 200 mg/kg. The HAME of L. 
      vulgaris (fruit) at dose of 200 mg/kg increased the sleeping time. The HAME and 
      HASE of L. vulgaris (seed) increased sleep duration at doses of 50 and 100 mg/kg. 
      Besides, flumazenil (2 mg/kg) reversed the effects of both diazepam (P < 0.001 
      vs. diazepam group), 200 mg/kg of HAME of C. pepo and 50 mg/kg of HAME and HASE 
      of L. vulgaris (seed). All fractions especially ethyl-acetate fraction (EAF) of 
      L. vulgaris (seed) increased the sleep duration. Naloxone reversed the hypnotic 
      effect of HAME and HASE of L. vulgaris (seed). The extracts showed no neurotoxic 
      effects on PC12 and L929 cell lines. Conclusion: The results showed that L. 
      vulgaris (seed and fruit) and C. pepo potentiated pentobarbital hypnosis without 
      toxic influence. The hypnotic effects of L. vulgaris seed was greater than its 
      fruit and C. pepo. The GABA and opioid receptors may play role in the 
      sleep-induction of L. vulgaris seed.
FAU - Baradaran Rahimi, Vafa
AU  - Baradaran Rahimi V
AD  - Student Research Committee, Department of Pharmacology, Faculty of Medicine, 
      Mashhad University of Medical Sciences, Mashhad 91778-99191, Iran. 
      baradaranv941@mums.ac.ir.
AD  - Pharmacological Research Center of Medicinal Plants, Mashhad University of 
      Medical Sciences, Mashhad 91778-99191, Iran. baradaranv941@mums.ac.ir.
FAU - Askari, Vahid Reza
AU  - Askari VR
AD  - Student Research Committee, Department of Pharmacology, Faculty of Medicine, 
      Mashhad University of Medical Sciences, Mashhad 91778-99191, Iran. 
      Askariv941@mums.ac.ir.
AD  - Pharmacological Research Center of Medicinal Plants, Mashhad University of 
      Medical Sciences, Mashhad 91778-99191, Iran. Askariv941@mums.ac.ir.
FAU - Tajani, Amineh Sadat
AU  - Tajani AS
AD  - Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of 
      Medical Sciences, Mashhad 91778-99191, Iran. TajaniA941@mums.ac.ir.
FAU - Hosseini, Azar
AU  - Hosseini A
AD  - Pharmacological Research Center of Medicinal Plants, Mashhad University of 
      Medical Sciences, Mashhad 91778-99191, Iran. HoseiniAZ@mums.ac.ir.
FAU - Rakhshandeh, Hassan
AU  - Rakhshandeh H
AD  - Student Research Committee, Department of Pharmacology, Faculty of Medicine, 
      Mashhad University of Medical Sciences, Mashhad 91778-99191, Iran. 
      Rakhshandehh@mums.ac.ir.
AD  - Pharmacological Research Center of Medicinal Plants, Mashhad University of 
      Medical Sciences, Mashhad 91778-99191, Iran. Rakhshandehh@mums.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20180731
PL  - Switzerland
TA  - Medicina (Kaunas)
JT  - Medicina (Kaunas, Lithuania)
JID - 9425208
RN  - 0 (Plant Extracts)
RN  - 0 (Sleep Aids, Pharmaceutical)
RN  - I4744080IR (Pentobarbital)
SB  - IM
MH  - Animals
MH  - *Cucurbita
MH  - Disease Models, Animal
MH  - Fruit
MH  - Male
MH  - Mice
MH  - Pentobarbital
MH  - Plant Extracts/administration & dosage/*pharmacology
MH  - Sleep/*drug effects
MH  - Sleep Aids, Pharmaceutical/administration & dosage/*pharmacology
MH  - Sleep Wake Disorders/*drug therapy
PMC - PMC6174352
OTO - NOTNLM
OT  - Cucurbita pepo
OT  - Lagenaria vulgaris
OT  - flumazenil
OT  - insomnia
OT  - naloxone
COIS- The authors declare no conflict of interest.
EDAT- 2018/10/23 06:00
MHDA- 2019/10/08 06:00
PMCR- 2018/07/31
CRDT- 2018/10/23 06:00
PHST- 2018/05/29 00:00 [received]
PHST- 2018/07/22 00:00 [revised]
PHST- 2018/07/26 00:00 [accepted]
PHST- 2018/10/23 06:00 [entrez]
PHST- 2018/10/23 06:00 [pubmed]
PHST- 2019/10/08 06:00 [medline]
PHST- 2018/07/31 00:00 [pmc-release]
AID - medicina54040055 [pii]
AID - medicina-54-00055 [pii]
AID - 10.3390/medicina54040055 [doi]
PST - epublish
SO  - Medicina (Kaunas). 2018 Jul 31;54(4):55. doi: 10.3390/medicina54040055.