PMID- 30344747
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 16
IP  - 5
DP  - 2018 Nov
TI  - Iodine-125 interstitial brachytherapy reduces tumor growth via Warburg effect 
      inhibition in non-small cell lung cancer A549 xenografts.
PG  - 5969-5977
LID - 10.3892/ol.2018.9346 [doi]
AB  - Iodine-125 interstitial brachytherapy (125I-IBT) is an alternative and effective 
      treatment option for unresectable non-small cell lung cancer (NSCLC), and the 
      Warburg effect is a determinant of tumor growth. The present study aimed to 
      explore the influence of 125I-IBT on tumor growth and the Warburg effect, and the 
      potential mechanisms underlying NSCLC progression. Mice with A549 cell xenografts 
      were evenly divided into a control group without 125I-IBT, and three treatment 
      groups receiving 125I-IBT with 20, 40 and 60 Gy. Tumor volume (TV), maximum 
      standardized uptake value (SUVmax) determined by 18F-fluorodeoxyglucose (18F-FDG) 
      micro-positron emission tomography/computed tomography and mean optical density 
      (MOD) of mammalian target of rapamycin (mTOR), c-Myc, hypoxia inducible factor-1alpha 
      (HIF-1alpha) and glucose transporter 1 (GLUT1) staining were compared among groups. 
      Tumor inhibition rate (TIR), 18F-FDG uptake attenuation rate (FUAR) and 
      expression suppression rate (ESR) were also calculated on day 14 and 28. The 
      results demonstrated that the mean TV in the 60 and 40 Gy groups was smaller 
      compared with the control TVs since days 14 and 16, respectively. The mean SUVmax 
      value of the 60 Gy group at day 14, and all treatment group SUVmax values at day 
      28 were lower compared with the controls. In addition, the MOD of mTOR and GLUT1 
      was lower in the 60 Gy group, compared with the other groups, and c-Myc and 
      HIF-1alpha values were lower in the 40 and 60 Gy groups, compared with the control 
      and 20 Gy group (P<0.05). SUVmax positively correlated to TV (day 14, r=0.711; 
      day 28, r=0.586) and the MOD of c-Myc and GLUT1 (r=0.621 and 0.546, respectively; 
      P<0.01). Furthermore, dose dependent increases were observed for TIR, FUAR and 
      ESR. In conclusion, 125I-IBT reduced tumor growth by inhibiting the Warburg 
      effect, which may have resulted from downregulation of mTOR, c-Myc, HIF-1alpha and 
      GLUT1 expression, particularly c-Myc and GLUT1, in NSCLC A549 xenografts.
FAU - Zhang, Jun
AU  - Zhang J
AD  - Department of Nuclear Medicine, The First Affiliated Hospital, College of 
      Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Zhu, Yangjun
AU  - Zhu Y
AD  - Department of Ultrasonography, The First Affiliated Hospital, College of 
      Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Dong, Mengjie
AU  - Dong M
AD  - Department of Nuclear Medicine, The First Affiliated Hospital, College of 
      Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Yang, Jun
AU  - Yang J
AD  - Department of Nuclear Medicine, The First Affiliated Hospital, College of 
      Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Weng, Wanwen
AU  - Weng W
AD  - Department of Nuclear Medicine, The First Affiliated Hospital, College of 
      Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Teng, Lisong
AU  - Teng L
AD  - Department of Surgical Oncology, The First Affiliated Hospital, College of 
      Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
AD  - Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and 
      Pancreatic Tumor of Zhejiang Province, The First Affiliated Hospital, College of 
      Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180821
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC6176348
OTO - NOTNLM
OT  - 18F-fluorodeoxyglucose
OT  - Warburg effect
OT  - brachytherapy
OT  - iodine-125 seed
OT  - micro-PET/CT
OT  - non-small cell lung cancer
EDAT- 2018/10/23 06:00
MHDA- 2018/10/23 06:01
PMCR- 2018/08/21
CRDT- 2018/10/23 06:00
PHST- 2018/03/26 00:00 [received]
PHST- 2018/08/10 00:00 [accepted]
PHST- 2018/10/23 06:00 [entrez]
PHST- 2018/10/23 06:00 [pubmed]
PHST- 2018/10/23 06:01 [medline]
PHST- 2018/08/21 00:00 [pmc-release]
AID - OL-0-0-9346 [pii]
AID - 10.3892/ol.2018.9346 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 Nov;16(5):5969-5977. doi: 10.3892/ol.2018.9346. Epub 2018 Aug 
      21.