PMID- 30345607
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1742-1241 (Electronic)
IS  - 1368-5031 (Linking)
DP  - 2018 Oct 21
TI  - Assessment of global long interspersed nucleotide element-1 (LINE-1) DNA 
      methylation in a longitudinal cohort of type 2 diabetes mellitus (T2DM) 
      individuals.
PG  - e13270
LID - 10.1111/ijcp.13270 [doi]
AB  - INTRODUCTION: Recent studies have indicated that methylation of the LINE-1 
      elements is associated with an increased risk of worsening carbohydrate 
      metabolism. It has been shown that overall DNA methylation of LINE-1 elements 
      could be considered as a risk factor for T2DM and its complications, independent 
      of other established risk factors. METHODS: A total of 794 T2DM individuals from 
      Salford, UK were included in this study (60% men n = 470). All patients had 
      clinical and metabolic variables measured in 2002 (baseline outcomes) and 
      annually through to 2016. Global LINE-1 DNA methylation was measured at four CpG 
      sites. The QIAGEN PyroMark Q96 MD pyrosequencer was used to quantify methylation. 
      RESULTS: The overall mean +/- SD global LINE-1 methylation was 75.81 +/- 3.25%. 
      Cross-sectional linear regression analysis at baseline year 2002 showed that 
      LINE-1 methylation was a significant predictor of diastolic BP (adjusted beta 
      coefficient beta = -0.25), estimated glomerular filtration rate (eGFR) (beta = -0.48) 
      and cholesterol HDL ratio (beta = -0.04). A 10% increase in LINE-1 methylation was 
      associated with a lower diastolic BP by 2.5 mm Hg, a lower eGFR by 
      4.8 ml/min/1.73 m(2) and decreased cholesterol/HDL ratio by 0.4 mmol/L. 
      Longitudinal analysis over the 14-year-follow-up periods showed that global 
      LINE-1 methylation at baseline was associated with lower BMI in women [beta = -0.25] 
      and lower cholesterol: HDL ratio [beta = -0.07]. A 10% increase in LINE-1 
      methylation was associated with reduction in BMI by 2.5 kg/m(2) in women and 
      reduction in cholesterol:HDL ratio by 0.7 mmol/L. CONCLUSION: In a 14-year 
      longitudinal cohort of T2DM individuals, relations between global LINE-1 DNA 
      methylation status and specific metabolic markers were seen. Also, a higher 
      degree of DNA methylation was predictive of less weight gain over time in women.
CI  - (c) 2018 John Wiley & Sons Ltd.
FAU - Malipatil, Nagaraj
AU  - Malipatil N
AD  - The School of Medicine and Manchester Academic Health Sciences Centre, University 
      of Manchester, Manchester, UK.
AD  - Department of Diabetes and Endocrinology, Salford Royal Hospital, Salford, UK.
FAU - Lunt, Mark
AU  - Lunt M
AD  - Department of Diabetes and Endocrinology, Salford Royal Hospital, Salford, UK.
FAU - Narayanan, Ram Prakash
AU  - Narayanan RP
AD  - Institute for Ageing and Chronic Diseases, University of Liverpool, Liverpool, 
      UK.
FAU - Siddals, Kirk
AU  - Siddals K
AD  - The School of Medicine and Manchester Academic Health Sciences Centre, University 
      of Manchester, Manchester, UK.
AD  - Department of Diabetes and Endocrinology, Salford Royal Hospital, Salford, UK.
FAU - Cortes Moreno, Gabriela Y
AU  - Cortes Moreno GY
AD  - High Speciality Regional Hospital of Ixtapaluca, Mexico City, Mexico.
FAU - Gibson, Martin J
AU  - Gibson MJ
AD  - Department of Diabetes and Endocrinology, Salford Royal Hospital, Salford, UK.
AD  - NorthWest EHealth Ltd, Manchester, UK.
FAU - Gu, Harvest F
AU  - Gu HF
AD  - Center for Molecular Medicine, Karolinska University Hospital, Solna, Stockholm, 
      Sweden.
FAU - Heald, Adrian H
AU  - Heald AH
AUID- ORCID: 0000-0002-9537-4050
AD  - The School of Medicine and Manchester Academic Health Sciences Centre, University 
      of Manchester, Manchester, UK.
AD  - Department of Diabetes and Endocrinology, Salford Royal Hospital, Salford, UK.
FAU - Donn, Rachelle P
AU  - Donn RP
AD  - The School of Medicine and Manchester Academic Health Sciences Centre, University 
      of Manchester, Manchester, UK.
LA  - eng
PT  - Journal Article
DEP - 20181021
PL  - India
TA  - Int J Clin Pract
JT  - International journal of clinical practice
JID - 9712381
EDAT- 2018/10/23 06:00
MHDA- 2018/10/23 06:00
CRDT- 2018/10/23 06:00
PHST- 2018/04/15 00:00 [received]
PHST- 2018/09/06 00:00 [accepted]
PHST- 2018/10/23 06:00 [entrez]
PHST- 2018/10/23 06:00 [pubmed]
PHST- 2018/10/23 06:00 [medline]
AID - 10.1111/ijcp.13270 [doi]
PST - aheadofprint
SO  - Int J Clin Pract. 2018 Oct 21:e13270. doi: 10.1111/ijcp.13270.