PMID- 30347083
OWN - NLM
STAT- MEDLINE
DCOM- 20190506
LR  - 20200309
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 59
IP  - 12
DP  - 2018 Oct 1
TI  - Structure-Function Changes of the Porcine Distal Outflow Tract in Response to 
      Nitric Oxide.
PG  - 4886-4895
LID - 10.1167/iovs.18-24943 [doi]
AB  - PURPOSE: To correlate outflow function and outflow tract vessel diameter changes 
      induced by nitric oxide (NO). METHODS: In a porcine anterior segment perfusion 
      model, the effects of a nitric oxide donor (100 muM DETA-NO) on outflow facility 
      were compared with controls (n = 8 per group) with trabecular meshwork (TM) and 
      after circumferential ab interno trabeculectomy (AIT). Outflow structures were 
      assessed with spectral-domain optical coherence tomography (SD-OCT) before and 
      after NO, or an NO synthase inhibitor (100 muM L-NAME) and the vasoconstrictor, 
      endothelin-1 (100 pg/mL ET-1). Scans were processed with a custom macroscript and 
      aligned for automated reslicing and quantification of cross-sectional outflow 
      tract areas (CSA). RESULTS: The facility increased after DETA-NO (Delta of 0.189 +/- 
      0.081 muL/min.mm Hg, P = 0.034) and AIT (Delta of 0.251 +/- 0.094 muL/min.mm Hg, P = 
      0.009), respectively. Even after AIT, DETA-NO increased the facility by 61.5% (Delta 
      of 0.190 +/- 0.074 muL/min.mm Hg, P = 0.023) and CSA by 13.9% (P < 0.001). L-NAME + 
      ET-1 decreased CSA by -8.6% (P < 0.001). NO increased the diameter of focal 
      constrictions 5.0 +/- 3.8-fold. CONCLUSIONS: NO can dilate vessels of the distal 
      outflow tract and increase outflow facility in a TM-independent fashion. There 
      are short, focally constricting vessel sections that display large diameter 
      changes and may have a substantial impact on outflow.
FAU - Waxman, Susannah
AU  - Waxman S
AD  - Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, 
      Pennsylvania, United States.
FAU - Wang, Chao
AU  - Wang C
AD  - Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, 
      Pennsylvania, United States.
AD  - Department of Ophthalmology, Xiangya Hospital, Central South University, 
      Changsha, Hunan, China.
AD  - The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.
FAU - Dang, Yalong
AU  - Dang Y
AD  - Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, 
      Pennsylvania, United States.
FAU - Hong, Ying
AU  - Hong Y
AD  - Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, 
      Pennsylvania, United States.
AD  - Department of Ophthalmology, Peking University Third Hospital, Beijing, China.
FAU - Esfandiari, Hamed
AU  - Esfandiari H
AD  - Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, 
      Pennsylvania, United States.
FAU - Shah, Priyal
AU  - Shah P
AD  - Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, 
      Pennsylvania, United States.
FAU - Lathrop, Kira L
AU  - Lathrop KL
AD  - Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, 
      Pennsylvania, United States.
FAU - Loewen, Ralitsa T
AU  - Loewen RT
AD  - Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, 
      Pennsylvania, United States.
FAU - Loewen, Nils A
AU  - Loewen NA
AD  - Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, 
      Pennsylvania, United States.
LA  - eng
GR  - K08 EY022737/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Endothelin-1)
RN  - 0 (Endothelium-Dependent Relaxing Factors)
RN  - 0 (Enzyme Inhibitors)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Aqueous Humor/*physiology
MH  - Endothelin-1/pharmacology
MH  - Endothelium-Dependent Relaxing Factors/*pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Intraocular Pressure/physiology
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Nitric Oxide/*pharmacology
MH  - Swine
MH  - Tomography, Optical Coherence
MH  - Trabecular Meshwork/diagnostic imaging/*drug effects/physiopathology
MH  - Trabeculectomy
PMC - PMC6181305
EDAT- 2018/10/23 06:00
MHDA- 2019/05/07 06:00
PMCR- 2018/10/01
CRDT- 2018/10/23 06:00
PHST- 2018/10/23 06:00 [entrez]
PHST- 2018/10/23 06:00 [pubmed]
PHST- 2019/05/07 06:00 [medline]
PHST- 2018/10/01 00:00 [pmc-release]
AID - 2707233 [pii]
AID - IOVS-18-24943R2 [pii]
AID - 10.1167/iovs.18-24943 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2018 Oct 1;59(12):4886-4895. doi: 
      10.1167/iovs.18-24943.