PMID- 30347698
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231004
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 8
IP  - 10
DP  - 2018 Oct 19
TI  - Stereoselective Double Reduction of 3-Methyl-2-cyclohexenone, by Use of Palladium 
      and Platinum Nanoparticles, in Tandem with Alcohol Dehydrogenase.
LID - 10.3390/nano8100853 [doi]
LID - 853
AB  - The combination of metal nanoparticles (Pd or Pt NPs) with NAD-dependent 
      thermostable alcohol dehydrogenase (TADH) resulted in the one-flask catalytic 
      double reduction of 3-methyl-2-cyclohexenone to 3-(1S,3S)-methylcyclohexanol. In 
      this article, some assumptions about the interactions between a chemocatalyst and 
      a biocatalyst have been proposed. It was demonstrated that the size of the NPs 
      was the critical parameter for the mutual inhibition: the bigger the NPs, the 
      more harmful for the enzyme they were, even if the NPs themselves were only 
      moderately inactivated. Conversely, the smaller the NPs, the more minimal the 
      TADH denaturation, although they were dramatically inhibited. Resuming, the 
      chemocatalysts were very sensitive to deactivation, which was not related to the 
      amount of enzyme used, while the inhibition of the biocatalyst can be strongly 
      reduced by minimizing the NPs/TADH ratio used to catalyze the reaction. Among 
      some methods to avoid direct binding of NPs with TADH, we found that using large 
      Pd NPs and protecting their surfaces with a silica shell, the overall yield of 
      3-(1S,3S)-methylcyclohexanol was maximized (36%).
FAU - Coccia, Francesca
AU  - Coccia F
AD  - Department of Engineering and Geology (INGEO), G. d'Annunzio University of 
      Chieti-Pescara, Viale Pindaro 42, I-66100 Chieti Scalo, Italy. 
      frasmile.f@libero.it.
AD  - Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 
      2629HZ Delft, The Netherlands. frasmile.f@libero.it.
FAU - Tonucci, Lucia
AU  - Tonucci L
AD  - Department of Philosophical, Educational and Economic Sciences, G. d'Annunzio 
      University of Chieti-Pescara, Via dei Vestini 31, I-66100 Chieti Scalo, Italy. 
      lucia.tonucci@unich.it.
FAU - Del Boccio, Piero
AU  - Del Boccio P
AUID- ORCID: 0000-0003-1653-2194
AD  - Department of Pharmacy, G. d'Annunzio University of Chieti-Pescara, Via dei 
      Vestini 31, I-66100 Chieti Scalo, Italy. piero.delboccio@unich.it.
FAU - Caporali, Stefano
AU  - Caporali S
AUID- ORCID: 0000-0002-5673-0462
AD  - Department of Chemistry, University of Firenze, Via della Lastruccia 3-13, 
      I-50019 Sesto Fiorentino, Italy. stefano.caporali@unifi.it.
FAU - Hollmann, Frank
AU  - Hollmann F
AD  - Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 
      2629HZ Delft, The Netherlands. f.hollmann@tudelft.nl.
FAU - d'Alessandro, Nicola
AU  - d'Alessandro N
AD  - Department of Engineering and Geology (INGEO), G. d'Annunzio University of 
      Chieti-Pescara, Viale Pindaro 42, I-66100 Chieti Scalo, Italy. 
      nicola.dalessandro@unich.it.
LA  - eng
PT  - Journal Article
DEP - 20181019
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC6215098
OTO - NOTNLM
OT  - alcohol dehydrogenase
OT  - biocatalysis
OT  - chemoenzymatic catalysis
OT  - nanocatalysis
OT  - nanoparticles
OT  - palladium
OT  - platinum
OT  - tandem reaction
COIS- The authors declare no conflict of interest.
EDAT- 2018/10/24 06:00
MHDA- 2018/10/24 06:01
PMCR- 2018/10/19
CRDT- 2018/10/24 06:00
PHST- 2018/09/21 00:00 [received]
PHST- 2018/10/12 00:00 [revised]
PHST- 2018/10/17 00:00 [accepted]
PHST- 2018/10/24 06:00 [entrez]
PHST- 2018/10/24 06:00 [pubmed]
PHST- 2018/10/24 06:01 [medline]
PHST- 2018/10/19 00:00 [pmc-release]
AID - nano8100853 [pii]
AID - nanomaterials-08-00853 [pii]
AID - 10.3390/nano8100853 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2018 Oct 19;8(10):853. doi: 10.3390/nano8100853.