PMID- 30348987
OWN - NLM
STAT- MEDLINE
DCOM- 20191016
LR  - 20191016
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Oct 22
TI  - In vitro rejuvenation of brain mitochondria by the inhibition of actin 
      polymerization.
PG  - 15585
LID - 10.1038/s41598-018-34006-5 [doi]
LID - 15585
AB  - The oxygen consumption rate (OCR) and cytochrome c oxidase (CcO) activity of 
      respiratory complex IV (CIV) in brain mitochondria significantly decline in 
      middle-aged male mice compared to younger male mice. To explore the mechanisms 
      underlying the regulation of brain mitochondrial function, we examined 
      CIV-associated proteins, and identified actin inside the isolated brain 
      mitochondria. Inhibiting actin polymerization using cytochalasin B (CB) 
      significantly enhanced the OCR and CcO activity of CIV in the mitochondria. These 
      changes were accompanied by a significant reduction in the amount of CIV-bound 
      cytochrome c (cyt c). Actin was also associated with respiratory complex III 
      (CIII); however, the amount of CIII-bound cyt c increased significantly after 
      treatment of the mitochondria with CB. In contrast, no significant alteration in 
      the assembly or the CcO activity of CIV in CIV-containing supercomplexes or CIV 
      monomers was induced by CB. These results suggest that mitochondrial actin plays 
      a crucial role in the regulation of the CcO activity and OCR of CIV with 
      modification of the retention of cyt c between CIV and CIII.
FAU - Takahashi, Kazuhide
AU  - Takahashi K
AD  - Biological Process of Aging, Tokyo Metropolitan Institute of Gerontology, 
      Itabashi, Tokyo, 173-0015, Japan.
FAU - Miura, Yuri
AU  - Miura Y
AD  - Proteome Research, Tokyo Metropolitan Institute of Gerontology, Itabashi, Tokyo, 
      173-0015, Japan.
FAU - Ohsawa, Ikuroh
AU  - Ohsawa I
AD  - Biological Process of Aging, Tokyo Metropolitan Institute of Gerontology, 
      Itabashi, Tokyo, 173-0015, Japan.
FAU - Shirasawa, Takuji
AU  - Shirasawa T
AD  - Shirasawa Anti-Aging Medical Institute, Bunkyo, Tokyo, 113-0033, Japan.
FAU - Takahashi, Mayumi
AU  - Takahashi M
AD  - Biological Process of Aging, Tokyo Metropolitan Institute of Gerontology, 
      Itabashi, Tokyo, 173-0015, Japan. mmtaka@tmig.or.jp.
LA  - eng
PT  - Journal Article
DEP - 20181022
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Actins)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Actins/antagonists & inhibitors/*metabolism
MH  - Animals
MH  - Brain/growth & development/*metabolism
MH  - Cytochalasin B/pharmacology
MH  - Electron Transport Complex IV/metabolism
MH  - Male
MH  - Mice
MH  - Mitochondria/drug effects/*metabolism
MH  - Oxygen Consumption
MH  - Polymerization
PMC - PMC6197264
COIS- The authors declare no competing interests.
EDAT- 2018/10/24 06:00
MHDA- 2019/10/17 06:00
PMCR- 2018/10/22
CRDT- 2018/10/24 06:00
PHST- 2018/05/17 00:00 [received]
PHST- 2018/10/07 00:00 [accepted]
PHST- 2018/10/24 06:00 [entrez]
PHST- 2018/10/24 06:00 [pubmed]
PHST- 2019/10/17 06:00 [medline]
PHST- 2018/10/22 00:00 [pmc-release]
AID - 10.1038/s41598-018-34006-5 [pii]
AID - 34006 [pii]
AID - 10.1038/s41598-018-34006-5 [doi]
PST - epublish
SO  - Sci Rep. 2018 Oct 22;8(1):15585. doi: 10.1038/s41598-018-34006-5.