PMID- 3034977
OWN - NLM
STAT- MEDLINE
DCOM- 19870710
LR  - 20191210
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 79
IP  - 6
DP  - 1987 Jun
TI  - Sodium-potassium pump, ion fluxes, and cellular dehydration in sickle cell 
      anemia.
PG  - 1621-8
AB  - We studied the role of the sodium-potassium pump in erythrocytes of 12 patients 
      with sickle cell anemia (SS). Ouabain-binding sites per cell and pump-mediated 
      Rb/K uptake were significantly higher in SS patients than in white or black 
      controls. Ouabain-resistant Rb/K influx was also greater than in normal controls 
      or patients with sickle cell trait. Deoxygenation of SS erythrocytes increased 
      ouabain-sensitive Rb/K influx without altering ouabain binding, presumably as the 
      consequence of an increase in the passive influx of sodium. Deoxygenation 
      increased mean corpuscular hemoglobin concentration (MCHC) by 5.5%, and studies 
      of the density distribution of SS cells indicated an increase in highly dense 
      fractions known to contain sickled erythrocytes. Ouabain prevented the rise in 
      MCHC and reduced the percentage of dense cells. These findings indicate a 
      magnified role for the sodium-potassium pump in the pathophysiology of SS 
      erythrocytes and suggest that its inhibition might prove useful in therapy.
FAU - Izumo, H
AU  - Izumo H
FAU - Lear, S
AU  - Lear S
FAU - Williams, M
AU  - Williams M
FAU - Rosa, R
AU  - Rosa R
FAU - Epstein, F H
AU  - Epstein FH
LA  - eng
GR  - AM-18078/AM/NIADDK NIH HHS/United States
GR  - HL-15157/HL/NHLBI NIH HHS/United States
GR  - RR-01032/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Receptors, Drug)
RN  - 0 (cardiac glycoside receptors)
RN  - 5ACL011P69 (Ouabain)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
RN  - MLT4718TJW (Rubidium)
RN  - RWP5GA015D (Potassium)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Anemia, Sickle Cell/*metabolism
MH  - Body Water/metabolism
MH  - Erythrocyte Indices
MH  - Erythrocytes/*metabolism
MH  - Humans
MH  - Middle Aged
MH  - Ouabain/metabolism/pharmacology
MH  - Oxygen/blood
MH  - Potassium/*metabolism
MH  - Receptors, Drug/analysis
MH  - Rubidium/metabolism
MH  - Sodium/*metabolism
MH  - Sodium-Potassium-Exchanging ATPase/antagonists & inhibitors/*metabolism
PMC - PMC424484
EDAT- 1987/06/01 00:00
MHDA- 1987/06/01 00:01
PMCR- 1987/06/01
CRDT- 1987/06/01 00:00
PHST- 1987/06/01 00:00 [pubmed]
PHST- 1987/06/01 00:01 [medline]
PHST- 1987/06/01 00:00 [entrez]
PHST- 1987/06/01 00:00 [pmc-release]
AID - 10.1172/JCI112998 [doi]
PST - ppublish
SO  - J Clin Invest. 1987 Jun;79(6):1621-8. doi: 10.1172/JCI112998.