PMID- 30352986
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231005
IS  - 1999-4923 (Print)
IS  - 1999-4923 (Electronic)
IS  - 1999-4923 (Linking)
VI  - 10
IP  - 4
DP  - 2018 Oct 24
TI  - Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of 
      Cell Seeding by the Design of Experiment.
LID - 10.3390/pharmaceutics10040200 [doi]
LID - 200
AB  - In this methodological paper, lyophilized fibroin-coated alginate microcarriers 
      (LFAMs) proposed as mesenchymal stem cells (MSCs) delivery systems and optimal 
      MSCs seeding conditions for cell adhesion rate and cell arrangement, was defined 
      by a Design of Experiment (DoE) approach. Cells were co-incubated with 
      microcarriers in a bioreactor for different time intervals and conditions: 
      variable stirring speed, dynamic culture intermittent or continuous, and 
      different volumes of cells-LFAMs loaded in the bioreactor. Intermittent dynamic 
      culture resulted as the most determinant parameter; the volume of LFAMs/cells 
      suspension and the speed used for the dynamic culture contributed as well, 
      whereas time was a less influencing parameter. The optimized seeding conditions 
      were: 98 min of incubation time, 12.3 RPM of speed, and 401.5 microL volume of 
      cells-LFAMs suspension cultured with the intermittent dynamic condition. This DoE 
      predicted protocol was then validated on both human Adipose-derived Stem Cells 
      (hASCs) and human Bone Marrow Stem Cells (hBMSCs), revealing a good cell adhesion 
      rate on the surface of the carriers. In conclusion, microcarriers can be used as 
      cell delivery systems at the target site (by injection or arthroscopic 
      technique), to maintain MSCs and their activity at the injured site for 
      regenerative medicine.
FAU - Perucca Orfei, Carlotta
AU  - Perucca Orfei C
AD  - IRCCS Istituto Ortopedico Galeazzi, Orthopaedic Biotechnology Lab, Via R. 
      Galeazzi 4, 20161 Milano, Italy. carlotta.perucca@grupposandonato.it.
FAU - Talo, Giuseppe
AU  - Talo G
AUID- ORCID: 0000-0003-1976-5073
AD  - IRCCS Istituto Ortopedico Galeazzi, Cells and Tissue Engineering Laboratory, Via 
      R. Galeazzi 4, 20161 Milano, Italy. giuseppe.talo@grupposandonato.it.
FAU - Vigano, Marco
AU  - Vigano M
AUID- ORCID: 0000-0002-6463-6564
AD  - IRCCS Istituto Ortopedico Galeazzi, Orthopaedic Biotechnology Lab, Via R. 
      Galeazzi 4, 20161 Milano, Italy. marco.vigano@grupposandonato.it.
FAU - Perteghella, Sara
AU  - Perteghella S
AUID- ORCID: 0000-0003-1128-7636
AD  - Department of Drug Sciences, University of Pavia, via T. Taramelli 12, 27100 
      Pavia, Italy. sara.perteghella@unipv.it.
FAU - Lugano, Gaia
AU  - Lugano G
AD  - IRCCS Istituto Ortopedico Galeazzi, Orthopaedic Biotechnology Lab, Via R. 
      Galeazzi 4, 20161 Milano, Italy. gaia.lugano@grupposandonato.it.
FAU - Fabro Fontana, Francesca
AU  - Fabro Fontana F
AD  - Department of Drug Sciences, University of Pavia, via T. Taramelli 12, 27100 
      Pavia, Italy. ffrancesca.fontana@gmail.com.
FAU - Ragni, Enrico
AU  - Ragni E
AD  - IRCCS Istituto Ortopedico Galeazzi, Orthopaedic Biotechnology Lab, Via R. 
      Galeazzi 4, 20161 Milano, Italy. enrico.ragni@grupposandonato.it.
FAU - Colombini, Alessandra
AU  - Colombini A
AUID- ORCID: 0000-0002-1800-3424
AD  - IRCCS Istituto Ortopedico Galeazzi, Orthopaedic Biotechnology Lab, Via R. 
      Galeazzi 4, 20161 Milano, Italy. alessandra.colombini@grupposandonato.it.
FAU - De Luca, Paola
AU  - De Luca P
AD  - IRCCS Istituto Ortopedico Galeazzi, Orthopaedic Biotechnology Lab, Via R. 
      Galeazzi 4, 20161 Milano, Italy. deluca.paola@grupposandonato.it.
FAU - Moretti, Matteo
AU  - Moretti M
AD  - IRCCS Istituto Ortopedico Galeazzi, Cells and Tissue Engineering Laboratory, Via 
      R. Galeazzi 4, 20161 Milano, Italy. matteo.moretti@grupposandonato.it.
FAU - Torre, Maria Luisa
AU  - Torre ML
AUID- ORCID: 0000-0001-8062-6836
AD  - Department of Drug Sciences, University of Pavia, via T. Taramelli 12, 27100 
      Pavia, Italy. marina.torre@unipv.it.
FAU - de Girolamo, Laura
AU  - de Girolamo L
AD  - IRCCS Istituto Ortopedico Galeazzi, Orthopaedic Biotechnology Lab, Via R. 
      Galeazzi 4, 20161 Milano, Italy. laura.degirolamo@grupposandonato.it.
LA  - eng
GR  - 42617604/Regione Lombardia/
PT  - Journal Article
DEP - 20181024
PL  - Switzerland
TA  - Pharmaceutics
JT  - Pharmaceutics
JID - 101534003
PMC - PMC6321597
OTO - NOTNLM
OT  - alginate
OT  - cell delivery
OT  - design of experiment
OT  - mesenchymal stem cells
OT  - microcarrier
OT  - one-step clinical procedure
OT  - silk fibroin
COIS- The authors declare no conflict of interest.
EDAT- 2018/10/26 06:00
MHDA- 2018/10/26 06:01
PMCR- 2018/12/01
CRDT- 2018/10/25 06:00
PHST- 2018/09/10 00:00 [received]
PHST- 2018/10/18 00:00 [revised]
PHST- 2018/10/22 00:00 [accepted]
PHST- 2018/10/25 06:00 [entrez]
PHST- 2018/10/26 06:00 [pubmed]
PHST- 2018/10/26 06:01 [medline]
PHST- 2018/12/01 00:00 [pmc-release]
AID - pharmaceutics10040200 [pii]
AID - pharmaceutics-10-00200 [pii]
AID - 10.3390/pharmaceutics10040200 [doi]
PST - epublish
SO  - Pharmaceutics. 2018 Oct 24;10(4):200. doi: 10.3390/pharmaceutics10040200.