PMID- 30356408
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 2314-436X (Print)
IS  - 2314-4378 (Electronic)
IS  - 2314-436X (Linking)
VI  - 2018
DP  - 2018
TI  - Transposable Elements in the Organization and Diversification of the Genome of 
      Aegilops speltoides Tausch (Poaceae, Triticeae).
PG  - 4373089
LID - 10.1155/2018/4373089 [doi]
LID - 4373089
AB  - Repetitive DNA-specifically, transposable elements (TEs)-is a prevailing genomic 
      fraction in cereals that underlies extensive genome reshuffling and intraspecific 
      diversification in the wild. Although large amounts of data have been 
      accumulated, the effect of TEs on the genome architecture and functioning is not 
      fully understood. Here, plant genome organization was addressed by means of 
      cloning and sequencing TE fragments of different types, which compose the largest 
      portion of the Aegilops speltoides genome. Individual genotypes were analyzed 
      cytogenetically using the cloned TE fragments as the DNA probes for fluorescence 
      in situ hybridization (FISH). The obtained TE sequences of the Ty1-copia, 
      Ty3-gypsy, LINE, and CACTA superfamilies showed the relatedness of the Ae. 
      speltoides genome to the Triticeae tribe and similarities to evolutionarily 
      distant species. A significant number of clones consisted of intercalated 
      fragments of TEs of various types, in which Fatima (Ty3-gypsy) sequences 
      predominated. At the chromosomal level, different TE clones demonstrated 
      sequence-specific patterning, emphasizing the effect of the TE fraction on the 
      Ae. speltoides genome architecture and intraspecific diversification. Altogether, 
      the obtained data highlight the current species-specific organization and 
      patterning of the mobile element fraction and point to ancient evolutionary 
      events in the genome of Ae. speltoides.
FAU - Raskina, Olga
AU  - Raskina O
AUID- ORCID: 0000-0003-4994-0856
AD  - Institute of Evolution University of Haifa, Aba-Hushi Avenue 199, Mount Carmel, 
      Haifa 498838, Israel.
LA  - eng
PT  - Journal Article
DEP - 20180926
PL  - United States
TA  - Int J Genomics
JT  - International journal of genomics
JID - 101605206
PMC - PMC6178165
EDAT- 2018/10/26 06:00
MHDA- 2018/10/26 06:01
PMCR- 2018/09/26
CRDT- 2018/10/26 06:00
PHST- 2017/12/29 00:00 [received]
PHST- 2018/08/19 00:00 [accepted]
PHST- 2018/10/26 06:00 [entrez]
PHST- 2018/10/26 06:00 [pubmed]
PHST- 2018/10/26 06:01 [medline]
PHST- 2018/09/26 00:00 [pmc-release]
AID - 10.1155/2018/4373089 [doi]
PST - epublish
SO  - Int J Genomics. 2018 Sep 26;2018:4373089. doi: 10.1155/2018/4373089. eCollection 
      2018.