PMID- 30357506
OWN - NLM
STAT- MEDLINE
DCOM- 20190311
LR  - 20220408
IS  - 1432-2048 (Electronic)
IS  - 0032-0935 (Linking)
VI  - 249
IP  - 3
DP  - 2019 Mar
TI  - Physical location of tandem repeats in the wheat genome and application for 
      chromosome identification.
PG  - 663-675
LID - 10.1007/s00425-018-3033-4 [doi]
AB  - A general distribution of tandem repeats (TRs) in the wheat genome was predicted 
      and a new web page combined with fluorescence in situ hybridization experiments, 
      and the newly developed Oligo probes will improve the resolution for wheat 
      chromosome identification. Comprehensive sequence analysis of tandem repeats (TR) 
      in the wheat reference genome permits discovery and application of TRs for 
      chromosome identification. Genome-wide localization of TRs was identified in the 
      reference sequences of Chinese Spring using Tandem Repeat Finder (TRF). A 
      database of repeats unit size, array number, and physical coverage length of TRs 
      in the wheat genome was built. The distribution of TRs occupied 3-5% of the wheat 
      chromosomes, with non-random dispersal across the A, B, and D genomes. Three 
      classes of TRs surrounding the predicted genes were compared. An optimized 
      computer-assisted website page B2DSC was constructed for the general distribution 
      and chromosomally enriched zones of TR sequences to be displayed graphically. The 
      physical distribution of predicted TRs in the wheat genome by B2DSC matched well 
      with the corresponding hybridization signals obtained with fluorescence in situ 
      hybridization (FISH). We developed 20 oligonucleotide probes representing 
      20-60 bp lengths of high copy number of TRs and verified by FISH. An integrated 
      physical map of TR-Oligo probes for wheat chromosome identification was 
      constructed. Our results suggest that the combination of both molecular 
      cytogenetics and genomic research will significantly benefit wheat breeding 
      through chromosome manipulation and engineering.
FAU - Lang, Tao
AU  - Lang T
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
FAU - Li, Guangrong
AU  - Li G
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
AD  - Center for Informational Biology, University of Electronic Science and Technology 
      of China, Chengdu, 611731, China.
FAU - Wang, Hongjin
AU  - Wang H
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
FAU - Yu, Zhihui
AU  - Yu Z
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
FAU - Chen, Qiheng
AU  - Chen Q
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
FAU - Yang, Ennian
AU  - Yang E
AD  - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 
      610066, Sichuan, China.
FAU - Fu, Shulan
AU  - Fu S
AD  - Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural 
      University, Chengdu, 611130, China.
FAU - Tang, Zongxiang
AU  - Tang Z
AD  - Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural 
      University, Chengdu, 611130, China.
FAU - Yang, Zujun
AU  - Yang Z
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China. yangzujun@uestc.edu.cn.
AD  - Center for Informational Biology, University of Electronic Science and Technology 
      of China, Chengdu, 611731, China. yangzujun@uestc.edu.cn.
LA  - eng
GR  - 2016YFD0102000/National Key Research and Development Program of China/
GR  - 31171542/National Natural Science Foundation of China/
GR  - 2016JY0075/Applied and Basic Project of Sichuan/
PT  - Journal Article
DEP - 20181024
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - *Chromosome Mapping
MH  - Chromosomes, Plant/*genetics
MH  - Genome, Plant/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Oligonucleotide Probes/genetics
MH  - Tandem Repeat Sequences/*genetics
MH  - Triticum/*genetics
OTO - NOTNLM
OT  - Chromosome identification
OT  - Fluorescence in situ hybridization
OT  - Tandem repeats
OT  - Wheat genome
EDAT- 2018/10/26 06:00
MHDA- 2019/03/12 06:00
CRDT- 2018/10/26 06:00
PHST- 2018/06/25 00:00 [received]
PHST- 2018/10/20 00:00 [accepted]
PHST- 2018/10/26 06:00 [pubmed]
PHST- 2019/03/12 06:00 [medline]
PHST- 2018/10/26 06:00 [entrez]
AID - 10.1007/s00425-018-3033-4 [pii]
AID - 10.1007/s00425-018-3033-4 [doi]
PST - ppublish
SO  - Planta. 2019 Mar;249(3):663-675. doi: 10.1007/s00425-018-3033-4. Epub 2018 Oct 
      24.