PMID- 30357941
OWN - NLM
STAT- MEDLINE
DCOM- 20190701
LR  - 20190816
IS  - 1600-0722 (Electronic)
IS  - 0909-8836 (Linking)
VI  - 126
IP  - 6
DP  - 2018 Dec
TI  - Eikenella corrodens lipopolysaccharide stimulates the pro-atherosclerotic 
      response in human coronary artery endothelial cells and monocyte adhesion.
PG  - 476-484
LID - 10.1111/eos.12580 [doi]
AB  - Eikenella corrodens is a gram-negative bacterium, and although primarily 
      associated with periodontal infections or infective endocarditis, it has been 
      identified in coronary atheromatous plaques. The effect of its lipopolysaccharide 
      (LPS) on human coronary artery endothelial cells (HCAECs) is unknown. Our aim was 
      to examine the mechanism underlying the inflammatory response in HCAECs 
      stimulated with E. corrodens-LPS and to evaluate monocyte adhesion. Endothelial 
      responses were determined by measuring the levels of chemokines and cytokines 
      using flow cytometry. The surface expression of intercellular adhesion molecule 1 
      (ICAM-1) was determined using a cell-based ELISA, and the adhesion of THP-1 
      monocytes to HCAECs was also monitored. The involvement of toll-like receptors 
      (TLRs) 2 and 4 was examined using TLR-neutralizing antibodies, and activation of 
      extracellular signal-regulated kinase (ERK)1/2 and nuclear factor-kappa B (NF-kappaB) 
      p65 were measured by western blotting and ELISA, respectively. 
      Eikenella corrodens-LPS increased secretion of interleukin-8 (IL-8), monocyte 
      chemotactic protein 1 (MCP-1), and granulocyte-macrophage colony-stimulating 
      factor (GM-CSF), and expression of ICAM-1 on the surface of HCAECs, consistent 
      with the increased adhesion of THP-1 cells. Moreover, E. corrodens-LPS interacted 
      with TLR4, a key receptor able to maintain the levels of IL-8, MCP-1, and GM-CSF 
      in HCAECs. Phosphorylation of ERK1/2 and activation of NF-kappaB p65 were also 
      increased. The results indicate that E. corrodens-LPS activates HCAECs through 
      TLR4, ERK, and NF-kappaB p65, triggering a pro-atherosclerotic endothelial response 
      and enhancing monocyte adhesion.
CI  - (c) 2018 Eur J Oral Sci.
FAU - Viafara-Garcia, Sergio M
AU  - Viafara-Garcia SM
AUID- ORCID: 0000-0002-4208-1422
AD  - Unit of Basic Oral Investigation UIBO, School of Dentistry, Universidad El 
      Bosque, Bogota, Colombia.
FAU - Gualtero, Diego F
AU  - Gualtero DF
AD  - Unit of Basic Oral Investigation UIBO, School of Dentistry, Universidad El 
      Bosque, Bogota, Colombia.
FAU - Avila-Ceballos, Daniel
AU  - Avila-Ceballos D
AD  - Unit of Basic Oral Investigation UIBO, School of Dentistry, Universidad El 
      Bosque, Bogota, Colombia.
FAU - Lafaurie, Gloria I
AU  - Lafaurie GI
AD  - Unit of Basic Oral Investigation UIBO, School of Dentistry, Universidad El 
      Bosque, Bogota, Colombia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181024
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (TLR2 protein, human)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Antibodies, Neutralizing
MH  - Cell Adhesion/drug effects
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Chemokines/metabolism
MH  - Coronary Artery Disease/*chemically induced/*immunology
MH  - Coronary Vessels/*drug effects
MH  - Cytokines/metabolism
MH  - Eikenella corrodens/*metabolism
MH  - Endothelial Cells/*drug effects
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interleukin-8/metabolism
MH  - Lipopolysaccharides/*adverse effects/*immunology/metabolism
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Monocytes/*drug effects
MH  - NF-kappa B/metabolism
MH  - Phosphorylation
MH  - THP-1 Cells/immunology
MH  - Toll-Like Receptor 2
MH  - Toll-Like Receptor 4/drug effects
OTO - NOTNLM
OT  - atherosclerosis
OT  - coronary artery disease
OT  - innate immunity
OT  - monocyte/macrophage
OT  - periodontitis
EDAT- 2018/10/26 06:00
MHDA- 2019/07/02 06:00
CRDT- 2018/10/26 06:00
PHST- 2018/10/26 06:00 [pubmed]
PHST- 2019/07/02 06:00 [medline]
PHST- 2018/10/26 06:00 [entrez]
AID - 10.1111/eos.12580 [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2018 Dec;126(6):476-484. doi: 10.1111/eos.12580. Epub 2018 Oct 
      24.