PMID- 30358017
OWN - NLM
STAT- MEDLINE
DCOM- 20190211
LR  - 20190215
IS  - 1740-0929 (Electronic)
IS  - 1344-3941 (Linking)
VI  - 90
IP  - 1
DP  - 2019 Jan
TI  - Significance of CCN2 expression in bovine preimplantation development.
PG  - 49-54
LID - 10.1111/asj.13126 [doi]
AB  - In mammalian preimplantation development, the first cell lineage segregation 
      occurs during the blastocyst stage, when the inner cell mass and trophectoderm 
      (TE) differentiate. Species-specific analyses are essential to elucidate the 
      molecular mechanisms that underlie this process, since they differ between 
      various species. We previously showed that the reciprocal regulation of CCN2 and 
      TEAD4 is required for proper TE differentiation in bovine blastocysts; however, 
      the function of CCN2 during early embryogenesis has remained otherwise elusive. 
      The present study assessed the spatiotemporal expression dynamics of CCN2 in 
      bovine embryos, and evaluated how changes to CCN2 expression (using a CCN2 
      knockdown (KD) blastocyst model) regulate the expression of pluripotency-related 
      genes such as OCT4 and NANOG. The conducted quantitative PCR analysis revealed 
      that CCN2 mRNA was expressed in bovine oocytes (at the metaphase stage of their 
      second meiosis) and embryos. Similarly, immunostaining detected both cytoplasmic 
      and nuclear CCN2 at all analyzed oocyte and embryonic stages. Finally, both OCT4 
      and NANOG expression levels were shown to be significantly reduced in CCN2 KD 
      blastocysts. Together, these results demonstrate that bovine CCN2 exhibits unique 
      expression patterns during preimplantation development, and is required for the 
      proper expression of key regulatory genes in bovine blastocysts.
CI  - (c) 2018 Japanese Society of Animal Science.
FAU - Akizawa, Hiroki
AU  - Akizawa H
AD  - Laboratory of Animal Breeding and Reproduction, Research Faculty of Agriculture, 
      Hokkaido University, Sapporo, Japan.
FAU - Yanagawa, Yojiro
AU  - Yanagawa Y
AD  - Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, 
      Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
FAU - Nagano, Masashi
AU  - Nagano M
AD  - Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, 
      Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
FAU - Bai, Hanako
AU  - Bai H
AD  - Laboratory of Animal Breeding and Reproduction, Research Faculty of Agriculture, 
      Hokkaido University, Sapporo, Japan.
FAU - Takahashi, Masashi
AU  - Takahashi M
AD  - Laboratory of Animal Breeding and Reproduction, Research Faculty of Agriculture, 
      Hokkaido University, Sapporo, Japan.
FAU - Kawahara, Manabu
AU  - Kawahara M
AD  - Laboratory of Animal Breeding and Reproduction, Research Faculty of Agriculture, 
      Hokkaido University, Sapporo, Japan.
LA  - eng
GR  - 18H02321/Grant-in-Aid for Scientific Research/
GR  - 17J01122/Grant-in-Aid for JSPS Fellows/
PT  - Journal Article
DEP - 20181024
PL  - Australia
TA  - Anim Sci J
JT  - Animal science journal = Nihon chikusan Gakkaiho
JID - 100956805
RN  - 0 (RNA, Messenger)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
SB  - IM
MH  - Animals
MH  - Blastocyst/metabolism/physiology
MH  - Cattle/*embryology/*genetics
MH  - Connective Tissue Growth Factor/*genetics/*metabolism/physiology
MH  - Embryonic Development/*genetics/physiology
MH  - Female
MH  - Fertilization in Vitro
MH  - Gene Expression/*genetics
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - In Vitro Oocyte Maturation Techniques
MH  - Oocytes/metabolism/physiology
MH  - RNA, Messenger/genetics/metabolism
OTO - NOTNLM
OT  - CCN2
OT  - blastocyst
OT  - bovine
OT  - cell-lineage specification
OT  - pluripotency-related genes
EDAT- 2018/10/26 06:00
MHDA- 2019/02/12 06:00
CRDT- 2018/10/26 06:00
PHST- 2018/06/22 00:00 [received]
PHST- 2018/08/10 00:00 [revised]
PHST- 2018/09/27 00:00 [accepted]
PHST- 2018/10/26 06:00 [pubmed]
PHST- 2019/02/12 06:00 [medline]
PHST- 2018/10/26 06:00 [entrez]
AID - 10.1111/asj.13126 [doi]
PST - ppublish
SO  - Anim Sci J. 2019 Jan;90(1):49-54. doi: 10.1111/asj.13126. Epub 2018 Oct 24.