PMID- 30359762
OWN - NLM
STAT- MEDLINE
DCOM- 20181218
LR  - 20181218
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 230
DP  - 2019 Feb 10
TI  - The ethanol extract of Involcucrum castaneae ameliorated ovalbumin-induced airway 
      inflammation and smooth muscle thickening in guinea pigs.
PG  - 9-19
LID - S0378-8741(18)32010-5 [pii]
LID - 10.1016/j.jep.2018.10.027 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Involucrum castaneae(IC)is used in Chinese folk 
      medicine to treat various lung diseases, as well as for its reducing phlegm and 
      anti-inflammatory properties. AIM OF THE STUDY: The purpose of this experiment is 
      to verify the effect of IC on airway inflammation, responsiveness in ovalbumin 
      (OVA)-induced asthmatic guinea pigs. The main chemical components of IC were also 
      analyzed. MATERIALS AND METHODS: The potential of the ethanol extract of 
      Involucrum castaneae (EEIC) to protect against OVA-induced allergic airway 
      response in guinea pigs was investigated. The latency of asthma in guinea pigs 
      were recorded after the allergic asthma induced. Enzyme-linked immunosorbent 
      assay (ELISA) was used to measure the levels of immunoglobulin E (IgE), 
      interleukin-5 (IL-5), nerve growth factor (NGF) and interferon-gamma (IFN-gamma) in 
      asthma allergy. Reverse transcription-PCR (RT-PCR) was used to detect the 
      expression of IL-5 mRNA in asthmatic guinea pig lungs. Paraffin sections of lung 
      tissue were used to analyze pathological changes. The total flavonoid content was 
      determined and the chemical components were analyzed by LC-MS/MS. RESULTS: It was 
      found that EEIC was able to reduce the number of eosinophil (EOS) in 
      bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) in the guinea pig 
      model of OVA -induced asthma. Meanwhile, it also significantly reduced the levels 
      of inflammation-related factors IgE and IL-5, decreased the expression of IL-5 
      mRNA in lung tissue, and increased the level of IFN-gamma. Pathological examination 
      of paraffin section of lung tissue showed that EEIC can reduce the thickening of 
      bronchial smooth muscle and reduce the infiltration damage of tissues by various 
      inflammatory cells. The presence of flavonoids, terpenoids and phenolic compounds 
      in EEIC might be responsible for these activities. CONCLUSION: IC alleviated 
      airway inflammation and smooth muscle thickening in guinea pigs with 
      OVA-sensitized allergic asthma. The paper explains the traditional efficacy and 
      material basis of IC and lays a foundation for further development.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Ye, Yujie
AU  - Ye Y
AD  - The Department of Pharmacy, College of Medicine, Wuhan University of Science and 
      Technology, Wuhan 430065, PR China.
FAU - Mo, Shuyuan
AU  - Mo S
AD  - College of Pharmacy, Huazhong University of Science and Technology, 13# Hangkong 
      Road, Wuhan 430030, PR China.
FAU - Feng, Wenya
AU  - Feng W
AD  - College of Pharmacy, Huazhong University of Science and Technology, 13# Hangkong 
      Road, Wuhan 430030, PR China.
FAU - Ye, Xiaochun
AU  - Ye X
AD  - Department of Pharmacy, the First Hospital of Wuhan, Wuhan 430022, PR China.
FAU - Shu, Xiang
AU  - Shu X
AD  - Department of Pharmacy, the First Hospital of Wuhan, Wuhan 430022, PR China.
FAU - Long, Yuxi
AU  - Long Y
AD  - College of Pharmacy, Huazhong University of Science and Technology, 13# Hangkong 
      Road, Wuhan 430030, PR China.
FAU - Guan, Yeli
AU  - Guan Y
AD  - College of Pharmacy, Huazhong University of Science and Technology, 13# Hangkong 
      Road, Wuhan 430030, PR China.
FAU - Huang, Jiangeng
AU  - Huang J
AD  - College of Pharmacy, Huazhong University of Science and Technology, 13# Hangkong 
      Road, Wuhan 430030, PR China.
FAU - Wang, Jianping
AU  - Wang J
AD  - College of Pharmacy, Huazhong University of Science and Technology, 13# Hangkong 
      Road, Wuhan 430030, PR China. Electronic address: jpwang1001@163.com.
LA  - eng
PT  - Journal Article
DEP - 20181023
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Allergens)
RN  - 0 (Anti-Asthmatic Agents)
RN  - 0 (Interleukin-5)
RN  - 0 (Plant Extracts)
RN  - 0 (Solvents)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 3K9958V90M (Ethanol)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Airway Remodeling/drug effects
MH  - Allergens
MH  - Animals
MH  - Anti-Asthmatic Agents/pharmacology/*therapeutic use
MH  - Asthma/chemically induced/*drug therapy/immunology/pathology
MH  - Ethanol/chemistry
MH  - *Fagaceae
MH  - Guinea Pigs
MH  - Immunoglobulin E/immunology
MH  - Interferon-gamma/immunology
MH  - Interleukin-5/genetics/immunology
MH  - Lung/drug effects/pathology
MH  - Male
MH  - Muscle, Smooth/drug effects/pathology
MH  - Ovalbumin
MH  - Plant Extracts/pharmacology/*therapeutic use
MH  - Solvents/chemistry
OTO - NOTNLM
OT  - Airway inflammation
OT  - Airway responsiveness
OT  - Anti-inflammatory
OT  - Involucrum castaneae
EDAT- 2018/10/26 06:00
MHDA- 2018/12/19 06:00
CRDT- 2018/10/26 06:00
PHST- 2018/06/28 00:00 [received]
PHST- 2018/10/11 00:00 [revised]
PHST- 2018/10/21 00:00 [accepted]
PHST- 2018/10/26 06:00 [pubmed]
PHST- 2018/12/19 06:00 [medline]
PHST- 2018/10/26 06:00 [entrez]
AID - S0378-8741(18)32010-5 [pii]
AID - 10.1016/j.jep.2018.10.027 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2019 Feb 10;230:9-19. doi: 10.1016/j.jep.2018.10.027. Epub 2018 
      Oct 23.