PMID- 30360042
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1976-8710 (Print)
IS  - 2005-0720 (Electronic)
IS  - 1976-8710 (Linking)
VI  - 12
IP  - 2
DP  - 2019 May
TI  - Effect of Ginkgo Biloba Extract on N-Methyl-D-Aspartic Acid Receptor Subunit 2B 
      Expression in a Salicylate-Induced Ototoxicity Model.
PG  - 169-175
LID - 10.21053/ceo.2018.00983 [doi]
AB  - OBJECTIVES: Sodium salicylate (SS) is well known for its ototoxic properties that 
      induce functional and morphological changes in the cochlea and brain. Ginkgo 
      biloba extract (GBE) has been widely used for treatment of various 
      neurodegenerative diseases; however, its effects on salicylate-induced 
      ototoxicity remain unclear. Herein, we examined the effects of EGb 761 (EGb), a 
      standard form of GBE, on the plasticity of the N-methyl-D-aspartate receptor 
      subunit 2B (GluN2B) in the inferior colliculus (IC) following SS administration. 
      METHODS: Seven-week-old Sprague Dawley rats (n=24) were randomly allocated to 
      control, SS, EGb, and EGb+SS groups. The SS group received a single 
      intraperitoneal SS injection (350 mg/kg), the EGb group received EGb orally for 5 
      consecutive days (40 mg/kg), and the EGb+SS group received EGb for 5 consecutive 
      days, followed by an SS injection. The auditory brainstem responses (ABRs) were 
      assessed at baseline and 2 hours after SS administration. GluN2B expression was 
      examined by Western blot and immunohistochemistry. RESULTS: There were no 
      significant differences in ABR threshold shifts among the groups. The expression 
      of the GluN2B protein normalized by which of glyceraldehyde 3-phosphate 
      dehydrogenase (GAPDH) was significantly lower in the EGb+SS group, as compared to 
      the SS group (P=0.012). Weak and diffused GluN2B immunoreactivity was detected in 
      the IC neural cells of the EGb+SS group, while those of the SS group exhibited 
      strong and diffused GluN2B positivity. CONCLUSION: EGb may play a role in 
      regulating the GluN2B expression in the IC of salicylate-induced ototoxicity 
      model.
FAU - Lee, Sang-Yeon
AU  - Lee SY
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, SMG-SNU Boramae Medical 
      Center, Seoul National University College of Medicine, Seoul, Korea.
FAU - Han, Sang Yoon
AU  - Han SY
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, SMG-SNU Boramae Medical 
      Center, Seoul National University College of Medicine, Seoul, Korea.
FAU - Shim, Ye-Ji
AU  - Shim YJ
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, SMG-SNU Boramae Medical 
      Center, Seoul National University College of Medicine, Seoul, Korea.
FAU - Han, Jae-Joon
AU  - Han JJ
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, SMG-SNU Boramae Medical 
      Center, Seoul National University College of Medicine, Seoul, Korea.
FAU - Cho, DeukTae
AU  - Cho D
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, SMG-SNU Boramae Medical 
      Center, Seoul National University College of Medicine, Seoul, Korea.
FAU - Kim, Ji Eun
AU  - Kim JE
AD  - Department of Pathology, SMG-SNU Boramae Medical Center, Seoul National 
      University College of Medicine, Seoul, Korea.
FAU - Kim, Young Ho
AU  - Kim YH
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, SMG-SNU Boramae Medical 
      Center, Seoul National University College of Medicine, Seoul, Korea.
LA  - eng
GR  - Seoul National University Boramae Medical Center/
PT  - Journal Article
DEP - 20181027
PL  - Korea (South)
TA  - Clin Exp Otorhinolaryngol
JT  - Clinical and experimental otorhinolaryngology
JID - 101474010
PMC - PMC6453795
OTO - NOTNLM
OT  - Ginkgo Biloba Extract
OT  - Inferior Colliculus
OT  - N-Methyl-D-Aspartate Receptor Subunit 2B
COIS- No potential conflict of interest relevant to this article was reported.
EDAT- 2018/10/27 06:00
MHDA- 2018/10/27 06:01
PMCR- 2019/05/01
CRDT- 2018/10/26 06:00
PHST- 2018/06/26 00:00 [received]
PHST- 2018/09/14 00:00 [accepted]
PHST- 2018/10/27 06:00 [pubmed]
PHST- 2018/10/27 06:01 [medline]
PHST- 2018/10/26 06:00 [entrez]
PHST- 2019/05/01 00:00 [pmc-release]
AID - ceo.2018.00983 [pii]
AID - ceo-2018-00983 [pii]
AID - 10.21053/ceo.2018.00983 [doi]
PST - ppublish
SO  - Clin Exp Otorhinolaryngol. 2019 May;12(2):169-175. doi: 10.21053/ceo.2018.00983. 
      Epub 2018 Oct 27.