PMID- 30363053
OWN - NLM
STAT- MEDLINE
DCOM- 20190619
LR  - 20211204
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 52
IP  - 5
DP  - 2018 Sep-Oct
TI  - [Methylation of the Reelin Gene Promoter in Peripheral Blood and Its Relationship 
      with the Cognitive Function of Schizophrenia Patients].
PG  - 782-792
LID - 10.1134/S0026898418050026 [doi]
AB  - There is a decrease in the expression of the reelin gene (RELN) in the brain of 
      schizophrenia patients, which can underlie observed cognitive abnormalities. It 
      is suggested that this decrease is caused by the hypermethylation of the RELN 
      promoter. The aim of the study was to investigate methylation of the RELN 
      promoter in the peripheral blood of schizophrenia patients and its association 
      with their cognitive deficits. A modified SMRT-BS (single-molecule real-time 
      bisulfite sequencing) was used. We determined the methylation rate of 170 CpG 
      sites within a 1465 bp DNA region containing the entire CpG island in the RELN 
      promoter in 51 schizophrenia patients and 52 healthy controls. All subjects 
      completed a battery of neuropsychological tests. There were no DNA methylation 
      changes associated with schizophrenia. Most CpGs sites were unmethylated in both 
      groups. At the same time, there was a variability in the methylation level of 
      different regions within the promoter. The methylation level in the area from 
      -258 to -151 bp relative to RELN transcription start site was a significant 
      predictor of the index of patients' cognitive functioning if sex, age, smoking, 
      education, and polymorphism rsl858815 had been considered. The positive 
      correlation between the methylation rate in this region and cognitive index 
      suggests that the hypomethylation of the RELN promoter could contribute to the 
      development of cognitive deficits in schizophrenia.
FAU - Alfimova, M V
AU  - Alfimova MV
AD  - Mental Health Research Center, Moscow, 115522 Russia.
FAU - Kondratiev, N V
AU  - Kondratiev NV
AD  - Mental Health Research Center, Moscow, 115522 Russia.
FAU - Golov, A K
AU  - Golov AK
AD  - Mental Health Research Center, Moscow, 115522 Russia.
FAU - Golimbet, V E
AU  - Golimbet VE
AD  - Mental Health Research Center, Moscow, 115522 Russia.
AD  - golimbet@mail.ru.
LA  - rus
PT  - Journal Article
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Reelin Protein)
RN  - EC 3.4.21.- (RELN protein, human)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Cell Adhesion Molecules, Neuronal/*genetics
MH  - *Cognition
MH  - CpG Islands
MH  - *DNA Methylation
MH  - Extracellular Matrix Proteins/*genetics
MH  - Female
MH  - Humans
MH  - Male
MH  - Nerve Tissue Proteins/*genetics
MH  - *Promoter Regions, Genetic
MH  - Reelin Protein
MH  - Schizophrenia/*genetics
MH  - Serine Endopeptidases/*genetics
MH  - Young Adult
OTO - NOTNLM
OT  - DNA methylation
OT  - RELN
OT  - cognitive deficit
OT  - schizophrenia
EDAT- 2018/10/27 06:00
MHDA- 2019/06/20 06:00
CRDT- 2018/10/27 06:00
PHST- 2017/10/18 00:00 [received]
PHST- 2018/02/15 00:00 [accepted]
PHST- 2018/10/27 06:00 [entrez]
PHST- 2018/10/27 06:00 [pubmed]
PHST- 2019/06/20 06:00 [medline]
AID - 10.1134/S0026898418050026 [doi]
PST - ppublish
SO  - Mol Biol (Mosk). 2018 Sep-Oct;52(5):782-792. doi: 10.1134/S0026898418050026.