PMID- 30364099
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 9
DP  - 2018
TI  - Burst-Like Subcutaneous Electrical Stimulation Induces BDNF-Mediated, Cyclotraxin 
      B-Sensitive Central Sensitization in Rat Spinal Cord.
PG  - 1143
LID - 10.3389/fphar.2018.01143 [doi]
LID - 1143
AB  - Intrathecal administration of brain derived neurotrophic factor (BDNF) induces 
      long-term potentiation (LTP) and generates long-lasting central sensitization in 
      spinal cord thus mimicking chronic pain, but the relevance of these observations 
      to chronic pain mechanisms is uncertain. Since C-fiber activation by a 
      high-frequency subcutaneous electrical stimulation (SES) protocol causes spinal 
      release of BDNF and induces spinal cord LTP, we propose that application of such 
      protocol would be a sufficient condition for generating long-lasting 
      BDNF-mediated central sensitization. Results showed that application of 
      burst-like SES to rat toes produced (i) rapid induction of hyperalgesia that 
      lasted for more than 3 weeks, (ii) early increase of C-reflex activity followed 
      by increased wind-up scores lasting for more than 1 week, and (iii) early 
      increase followed by late decrease in BDNF protein levels and phosphorylated TrkB 
      that lasted for more than 1 week. These changes were prevented by the TrkB 
      antagonist cyclotraxin-B administered shortly before SES, while hyperalgesia was 
      reversed by cyclotraxin-B administered 3 days after SES. Results suggest that 
      mechanisms underlying central sensitization first involve BDNF release of 
      probably neuronal origin, followed by brief increased expression of likely glial 
      BDNF and pTrkB that could switch early phase sensitization into late one.
FAU - Retamal, Jeffri
AU  - Retamal J
AD  - Laboratory of Neurobiology, Department of Biology, Faculty of Chemistry and 
      Biology, University of Santiago de Chile, Santiago, Chile.
AD  - Center for the Development of Nanoscience and Nanotechnology (CEDENNA), Santiago, 
      Chile.
FAU - Reyes, Andrea
AU  - Reyes A
AD  - Laboratory of Neurobiology, Department of Biology, Faculty of Chemistry and 
      Biology, University of Santiago de Chile, Santiago, Chile.
FAU - Ramirez, Paulina
AU  - Ramirez P
AD  - Laboratory of Neurobiology, Department of Biology, Faculty of Chemistry and 
      Biology, University of Santiago de Chile, Santiago, Chile.
AD  - Center for the Development of Nanoscience and Nanotechnology (CEDENNA), Santiago, 
      Chile.
FAU - Bravo, David
AU  - Bravo D
AD  - Laboratory of Neurobiology, Department of Biology, Faculty of Chemistry and 
      Biology, University of Santiago de Chile, Santiago, Chile.
AD  - Center for the Development of Nanoscience and Nanotechnology (CEDENNA), Santiago, 
      Chile.
FAU - Hernandez, Alejandro
AU  - Hernandez A
AD  - Laboratory of Neurobiology, Department of Biology, Faculty of Chemistry and 
      Biology, University of Santiago de Chile, Santiago, Chile.
FAU - Pelissier, Teresa
AU  - Pelissier T
AD  - Program of Molecular and Clinical Pharmacology, Institute of Biomedical Sciences, 
      Faculty of Medicine, University of Chile, Santiago, Chile.
FAU - Villanueva, Luis
AU  - Villanueva L
AD  - Centre de Psychiatrie et Neurosciences, INSERM UMR 894, Paris, France.
FAU - Constandil, Luis
AU  - Constandil L
AD  - Laboratory of Neurobiology, Department of Biology, Faculty of Chemistry and 
      Biology, University of Santiago de Chile, Santiago, Chile.
AD  - Center for the Development of Nanoscience and Nanotechnology (CEDENNA), Santiago, 
      Chile.
LA  - eng
PT  - Journal Article
DEP - 20181010
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC6191473
OTO - NOTNLM
OT  - C-reflex
OT  - brain-derived neurotrophic factor
OT  - cyclotraxin-B
OT  - hyperalgesia
OT  - subcutaneous electrical stimulation
OT  - windup
EDAT- 2018/10/27 06:00
MHDA- 2018/10/27 06:01
PMCR- 2018/10/10
CRDT- 2018/10/27 06:00
PHST- 2018/06/28 00:00 [received]
PHST- 2018/09/21 00:00 [accepted]
PHST- 2018/10/27 06:00 [entrez]
PHST- 2018/10/27 06:00 [pubmed]
PHST- 2018/10/27 06:01 [medline]
PHST- 2018/10/10 00:00 [pmc-release]
AID - 10.3389/fphar.2018.01143 [doi]
PST - epublish
SO  - Front Pharmacol. 2018 Oct 10;9:1143. doi: 10.3389/fphar.2018.01143. eCollection 
      2018.