PMID- 30365261 OWN - NLM STAT- MEDLINE DCOM- 20190408 LR - 20190408 IS - 1000-0607 (Print) IS - 1000-0607 (Linking) VI - 43 IP - 10 DP - 2018 Oct 25 TI - [Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats]. PG - 651-6 LID - 10.13702/j.1000-0607.180317 [doi] AB - OBJECTIVE: To observe the effect of electroacupuncture (EA) stimulation of single and multiple acupoints on sleep and concentrations of interlukin-1 beta(IL-1 beta), brain-derived neurotrophic factor (BDNF), prostaglandin D(2)(PGD(2)) and melatonin (MLT, sleep-promoting factors) and corticosterone (CORT, awakening-promoting factor) in the serum in insomnia rats, so as to explore its efficacy difference and the mechanism underlying improving sleep. METHODS: Fifty-four male SD rats were randomly divided into control, model, EA-Baihui (GV 20), EA-Shenmen (HT 7), EA-Sanyinjiao (SP 6) and EA-GV 20+HT 7+SP 6 groups (n=9 rats in each group). The insomnia model was established by intraperitoneal injection of para-chlorophenylalanine (PCPA, 300 mg/kg) once daily for 2 days. In the EA-GV 20, EA-HT 7, EA-SP 6 and EA-GV 20+HT 7+SP 6 groups, EA stimulation was administrated for 30 min, once a day for 4 days. The sleep onset latency and sleep duration were measured after intraperitoneal injection of pentobarbital sodium (35 mg/kg). The concentrations of IL-1 beta, BDNF, MLT, PGD(2)and CORT in the serum were detected by ELISA. RESULTS: After EA stimulation of GV 20, HT 7, SP 6 and GV 20+HT 7+SP 6, the sleep latency was significantly shortened (P<0.05, P<0.01, except SP 6), and the sleep duration was remarkably prolonged in comparison with the model group (P<0.05, P<0.01), and the therapeutic effects of EA-GV 20+HT 7+SP 6 were significantly superior to those of EA-GV 20, EA-HT 7 and EA-SP 6 in shortening the sleep latency and lengthening the sleep duration (P<0.05). Following modeling, the concentrations of IL-1 beta, BDNF, PGD(2) and MLT were significantly down-regulated, and the CORT level was markedly up-regulated in the model group relevant to the control group (P<0.05). Following EA,modeling induced dramatic decrease of serum IL-1 beta, BDNF, PGD(2) and MLT was considerably up-regulated, and the increased CORT level markedly down-regulated in the EA-GV 20, EA-HT 7, EA-SP 6 and EA-GV 20+HT 7+SP 6 groups (P<0.05). The effects of EA-GV 20+HT 7+SP 6 were evidently superior to those of EA-GV 20 and EA-SP 6 in up-regulating serum IL-1 beta, BDNF and PGD(2)levels, and to those of HT 7, GV 20 and SP 6 in up-regulating serum MLT level, and significantly superior to those of EA-ST 7 and EA-SP 6 in down-regulating serum CORT (P<0.05). CONCLUSION: EA stimulation of HT 7, GV 20, SP 6 and GV 20+HT 7+ SP 6 can significantly improve the sleep in insomnia rats, which is closely associated with its effects in regulating serum sleep-promoting factors and awakening-promoting factor. Joint administration of EA of GV 20+HT 7+ SP 6 has a better effect than the single acupoint mentioned above. FAU - Qiao, Li-Na AU - Qiao LN AD - Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China. FAU - Gao, Qiao-Ling AU - Gao QL AD - Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China; College of Acupuncture-moxibustion and Massage, Changchun University of Traditional Chinese Medicine, Changchun 130117. FAU - Tan, Lian-Hong AU - Tan LH AD - Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China. FAU - Shi, Yi-Nan AU - Shi YN AD - Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China. FAU - Jiang, Yan-Shu AU - Jiang YS AD - Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China; College of Acupuncture-moxibustion and Massage, Changchun University of Traditional Chinese Medicine, Changchun 130117. FAU - Yang, Jiao-Jiao AU - Yang JJ AD - College of Acupuncture-moxibustion and Massage, Changchun University of Traditional Chinese Medicine, Changchun 130117. FAU - Yang, Yong-Sheng AU - Yang YS AD - Lab of Biochemistry and Molecular Biology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China. LA - chi PT - Journal Article PL - China TA - Zhen Ci Yan Jiu JT - Zhen ci yan jiu = Acupuncture research JID - 8507710 RN - 0 (Brain-Derived Neurotrophic Factor) SB - IM MH - Acupuncture Points MH - Animals MH - Brain-Derived Neurotrophic Factor MH - *Electroacupuncture MH - Male MH - Rats MH - Rats, Sprague-Dawley MH - *Sleep Initiation and Maintenance Disorders OTO - NOTNLM OT - Acupoint combination OT - Awakening-promoting factor OT - Electroacupuncture OT - Insomnia OT - Peripheral blood OT - Sleep-promoting factors EDAT- 2018/10/27 06:00 MHDA- 2019/04/09 06:00 CRDT- 2018/10/27 06:00 PHST- 2018/10/27 06:00 [entrez] PHST- 2018/10/27 06:00 [pubmed] PHST- 2019/04/09 06:00 [medline] AID - 10.13702/j.1000-0607.180317 [doi] PST - ppublish SO - Zhen Ci Yan Jiu. 2018 Oct 25;43(10):651-6. doi: 10.13702/j.1000-0607.180317.