PMID- 30365624
OWN - NLM
STAT- MEDLINE
DCOM- 20190501
LR  - 20191210
IS  - 1678-4227 (Electronic)
IS  - 0004-282X (Linking)
VI  - 76
IP  - 9
DP  - 2018 Sep
TI  - The effect of CA1 administration of orexin-A on hippocampal expression of COX-2 
      and BDNF in a rat model of orofacial pain.
PG  - 603-608
LID - S0004-282X2018000900603 [pii]
LID - 10.1590/0004-282X20180099 [doi]
AB  - OBJECTIVE: The neuropeptide orexin-A and its receptors are widely distributed in 
      both hippocampal circuitry and pain transmission pathways. Involvement of the CA1 
      orexin 1 receptor (OX1R) on the modulation of orofacial pain and pain-induced 
      changes in hippocampal expression of cyclooxygenase-2 (COX-2) and brain-derived 
      neurotrophic factor (BDNF) was investigated. METHODS: Orofacial pain was induced 
      by an intra-lip injection of capsaicin (100 mug). Reverse transcription polymerase 
      chain reaction and immunoblot analysis were used to indicate changes in 
      hippocampal BDNF and COX-2 expression, respectively. RESULTS: Capsaicin induces a 
      significant pain response, which is not affected by either orexin-A or 
      SB-334867-A, an OX1R antagonist. However, an increased expression of COX-2 and 
      decreased expression of BDNF was observed in the hippocampus of animals that 
      received capsaicin or SB-334867-A (80 nM) plus capsaicin. Meanwhile, orexin-A (40 
      pM) attenuated the effects of capsaicin on the expression of COX-2 and BDNF. 
      CONCLUSIONS: CA1 OX1R activation moderates capsaicin-induced neuronal 
      inflammation and neurotrophic deficiency.
FAU - Kooshki, Razieh
AU  - Kooshki R
AD  - Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Biology, 
      Kerman, Iran.
FAU - Abbasnejad, Mehdi
AU  - Abbasnejad M
AD  - Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Biology, 
      Kerman, Iran.
FAU - Esmaeili-Mahani, Saeed
AU  - Esmaeili-Mahani S
AD  - Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Biology, 
      Kerman, Iran.
FAU - Raoof, Maryam
AU  - Raoof M
AD  - Kerman University of Medical Sciences, Institute of Neuropharmacology, 
      Neuroscience Research Center, Laboratory of Molecular Neuroscience, Kerman, Iran.
AD  - Kerman University of Medical Sciences, Endodontology Research Center, Kerman, 
      Iran.
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arq Neuropsiquiatr
JT  - Arquivos de neuro-psiquiatria
JID - 0125444
RN  - 0 (1-(2-methylbenzoxazol-6-yl)-3-(1,5)naphthyridin-4-yl urea)
RN  - 0 (Benzoxazoles)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Naphthyridines)
RN  - 0 (Orexin Receptors)
RN  - 0 (Orexins)
RN  - 8W8T17847W (Urea)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - S07O44R1ZM (Capsaicin)
SB  - IM
MH  - Animals
MH  - Benzoxazoles/pharmacology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Capsaicin
MH  - Cyclooxygenase 2/*metabolism
MH  - Disease Models, Animal
MH  - Facial Pain/*metabolism
MH  - Hippocampus/drug effects/*metabolism
MH  - Male
MH  - Naphthyridines
MH  - Neurons/drug effects/metabolism
MH  - Orexin Receptors/*metabolism
MH  - Orexins/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Urea/analogs & derivatives/pharmacology
EDAT- 2018/10/27 06:00
MHDA- 2019/05/02 06:00
CRDT- 2018/10/27 06:00
PHST- 2018/03/25 00:00 [received]
PHST- 2018/06/12 00:00 [accepted]
PHST- 2018/10/27 06:00 [entrez]
PHST- 2018/10/27 06:00 [pubmed]
PHST- 2019/05/02 06:00 [medline]
AID - S0004-282X2018000900603 [pii]
AID - 10.1590/0004-282X20180099 [doi]
PST - ppublish
SO  - Arq Neuropsiquiatr. 2018 Sep;76(9):603-608. doi: 10.1590/0004-282X20180099.