PMID- 30366199
OWN - NLM
STAT- MEDLINE
DCOM- 20181214
LR  - 20181214
IS  - 1618-0429 (Electronic)
IS  - 0932-4739 (Linking)
VI  - 66
DP  - 2018 Oct
TI  - Formation and degradation of large extrusion bodies in Tetrahymena thermophila: 
      The role of intramacronuclear microtubules in chromatin segregation.
PG  - 177-188
LID - S0932-4739(18)30037-3 [pii]
LID - 10.1016/j.ejop.2018.09.008 [doi]
AB  - Large extrusion bodies (EBs) in Tetrahymena thermophila were induced by treatment 
      with aphidiocolin (APH), followed by transfer of the cells to a drug free medium. 
      APH induces over-replication of DNA and reversible cell division arrest 
      (Kaczanowski and Kiersnowska, 2011). After treatment the cells were transferred 
      to a drug free medium, and a central granule of chromatin (a prospective EB) 
      surrounded by microtubules developed inside the macronucleus. Subsequently the 
      chromatin of the central granule appeared in the middle region of the dividing 
      macronucleus, and it was later extruded as an EB. The remaining chromatin 
      segregated to opposite ends of the elongating macronucleus and was separated from 
      the chromatin destined for the EB by intramacronuclear microtubules. TUNEL assay 
      (terminal deoxynucleotidyl transferase dUTP nick end labeling]) and in vivo 
      acridine orange (AO) staining showed that the EBs underwent apoptotic-like 
      degradation and autophagy similar to that observed in programmed nuclear death 
      (PND) of the old macronucleus in conjugant cells. Overall, based on the data 
      obtained in this study we proposed the hypothesis that over- replication of DNA 
      induced by APH results in the appearance of defective DNA copies, that are not 
      segregated to the opposite regions of dividing macronucleus and are destined for 
      EBs.
CI  - Copyright (c) 2018 Elsevier GmbH. All rights reserved.
FAU - Kaczanowski, Andrzej
AU  - Kaczanowski A
AD  - Institut of Zoology, Faculty of Biology, University of Warsaw, Miecznikowa str 1, 
      02-096 Warsaw, Poland. Electronic address: kaczan@biol.uw.edu.pl.
FAU - Kiersnowska, Mauryla
AU  - Kiersnowska M
AD  - Institut of Zoology, Faculty of Biology, University of Warsaw, Miecznikowa str 1, 
      02-096 Warsaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20180927
PL  - Germany
TA  - Eur J Protistol
JT  - European journal of protistology
JID - 8917383
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Chromatin)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Chromatin/drug effects/*metabolism
MH  - Macronucleus/genetics
MH  - Microtubules/*metabolism
MH  - Tetrahymena thermophila/drug effects/*genetics
OTO - NOTNLM
OT  - Chromatin segregation
OT  - Extrusion bodies
OT  - Macronuclear division
OT  - Minich romosomes
OT  - Tetrahymena thermophile
EDAT- 2018/10/27 06:00
MHDA- 2018/12/15 06:00
CRDT- 2018/10/27 06:00
PHST- 2018/04/29 00:00 [received]
PHST- 2018/09/19 00:00 [revised]
PHST- 2018/09/24 00:00 [accepted]
PHST- 2018/10/27 06:00 [pubmed]
PHST- 2018/12/15 06:00 [medline]
PHST- 2018/10/27 06:00 [entrez]
AID - S0932-4739(18)30037-3 [pii]
AID - 10.1016/j.ejop.2018.09.008 [doi]
PST - ppublish
SO  - Eur J Protistol. 2018 Oct;66:177-188. doi: 10.1016/j.ejop.2018.09.008. Epub 2018 
      Sep 27.